【摘要】：研究目的及背景： 以關(guān)節(jié)表面軟骨及關(guān)節(jié)內(nèi)游離體表面軟骨為研究對象，通過FT-IR光譜成像及相關(guān)分析方法，探索同處于一個關(guān)節(jié)腔微環(huán)境中兩種表面軟骨在分子水平的差異性，更深層次分析游離體表面軟骨生物學(xué)行為發(fā)生變化的特點，從光譜學(xué)角度研究游離體的形成因素及與關(guān)節(jié)炎的關(guān)系，探尋關(guān)節(jié)軟骨生長、分化規(guī)律并為臨床治療提供思路和理論依據(jù)。 資料與方法： 1.臨床資料吉林大學(xué)中日聯(lián)誼醫(yī)院關(guān)節(jié)外科2013年1月至2014年1月資料較完整的行髖膝關(guān)節(jié)置換術(shù)的患者作為實驗組樣本來源，術(shù)中可提取到關(guān)節(jié)內(nèi)游離體標(biāo)本。對照組樣本來源為同一關(guān)節(jié)腔內(nèi)的經(jīng)病理證實為正常的關(guān)節(jié)軟骨。其中髖關(guān)節(jié)骨性關(guān)節(jié)炎1例，膝關(guān)節(jié)骨性關(guān)節(jié)炎9例；男性4例，女性6例。所有患者均進(jìn)行髖膝部位正側(cè)位的放射學(xué)檢查，，用以明確診斷。所有樣本均進(jìn)行組織病理學(xué)檢查，確定組間的差異性。 2.方法所用樣本經(jīng)成像前處理之后經(jīng)FT-IR成像儀系統(tǒng)成像，收集各樣本的吸收譜線，并對譜線進(jìn)行進(jìn)一步處理和計算。 3.分析利用統(tǒng)計學(xué)軟件（SPSS17.0）對兩組的二階導(dǎo)數(shù)譜線波數(shù)位置進(jìn)行統(tǒng)計學(xué)分析；選取所有樣本的膠原及蛋白多糖波數(shù)及其對應(yīng)的紅外吸收值組成矩陣進(jìn)行主成分分析。 結(jié)果： 1.兩種軟骨中膠原分子的N-H彎曲振動波數(shù)位置對照組為1514cm-1，實驗組表現(xiàn)出明顯藍(lán)移，為1516cm-1。 2.兩種軟骨歸屬為酰胺II的N-H彎曲振動和C-N的拉伸震動波數(shù)對照組為1573cm-1，實驗組表現(xiàn)出明顯的藍(lán)移，波數(shù)位置為1575cm-1。 3.通過間接衡量兩種樣本的軟骨中蛋白多糖總成分含量，兩樣本無差異。 4.主成分分析法發(fā)現(xiàn)兩者在主成分坐標(biāo)系的散點圖中分布有差異性。 結(jié)論： 1.游離體表面軟骨的膠原成分較關(guān)節(jié)表面軟骨的分子更加穩(wěn)定，表明游離體表面軟骨成分中起骨架支撐作用膠原更不容易被膠原酶所分解； 2.同處于一個微環(huán)境下的關(guān)節(jié)表面軟骨和游離體表面軟骨的PG總成分相同，表明PG作為填充物未出現(xiàn)質(zhì)的變化； 3.在膠原和蛋白多糖的成分組成上兩者是有區(qū)別的，游離體表面軟骨在成分上表現(xiàn)出明顯的缺陷性。
[Abstract]:Objective and background: the articular surface cartilage and the articular free body surface cartilage were studied by FT-IR spectral imaging and correlation analysis. To explore the differences between the two kinds of surface cartilage at molecular level in the same microenvironment of articular cavity, and to further analyze the characteristics of the biological behavior of free surface cartilage. The formation factors of free body and the relationship between free body and arthritis were studied from the point of view of spectroscopy, the growth and differentiation of articular cartilage were explored and the theoretical basis for clinical treatment was provided. Data and methods: 1. Clinical data from January 2013 to January 2014, the patients undergoing hip and knee arthroplasty in Jilin University Sino-Japanese Friendship Hospital from January 2013 to January 2014 were selected as experimental group samples, and intraarticular free body specimens could be extracted during the operation. The control group came from the same articular cavity, pathologically proved to be normal articular cartilage. One case of hip osteoarthritis, 9 cases of knee osteoarthritis, male 4 cases, female 6 cases. All patients underwent radiographic examination of the hip and knee. All samples were examined by histopathology to determine the difference between groups. 2. 2. Methods the absorption lines of the samples were collected by FT-IR imaging system after pre-processing, and the spectral lines were further processed and calculated. Statistical software (SPSS17.0) was used to analyze the position of the second derivative line wavenumber of the two groups, and the wave numbers of collagen and proteoglycan and their corresponding infrared absorption values were selected for principal component analysis. Results: 1. The N-H bending vibrational wavenumber position of collagen molecules in the two kinds of cartilage was 1514cm-1 in the control group and 1516cm-1.2 in the experimental group. The wave number of N-H bending vibration and C-N tensile vibration were 1573 cm ~ (-1) and 1575 cm ~ (-1), respectively, which belonged to amide II, and the position of wave number was 1575 cm ~ (-1) in the experimental group. The total content of proteoglycan in cartilage of two kinds of samples was measured indirectly, and there was no difference between the two samples. 4. Principal component Analysis (PCA) shows that there are differences between them in scattered plot of principal component coordinate system. Conclusion: 1. The collagen composition of free cartilage is more stable than that of articular cartilage, which indicates that collagen is less likely to be decomposed by collagenase. The total composition of PG in articular surface cartilage and free body surface cartilage was the same as that in a microenvironment, indicating that there was no qualitative change in PG as filler. 3. The composition of collagen and proteoglycan were different, and the free surface cartilage showed obvious defect in composition.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R684

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 裴明,曲綿域,于長隆,蔣青;骨關(guān)節(jié)炎關(guān)節(jié)軟骨及游離體中膠原ⅠⅡⅢ型的表達(dá)[J];中華風(fēng)濕病學(xué)雜志;2000年02期

2 徐斌;徐洪港;;膝關(guān)節(jié)軟骨游離體的組織形態(tài)及其生理學(xué)特性[J];中國組織工程研究與臨床康復(fù);2007年06期

本文編號：2231775