【摘要】：目的:比較右美托咪定(dexmedetomidine,DEX)、丙泊酚(propofol,PRO)預(yù)處理對(duì)大鼠腸缺血/再灌注損傷(intestinal ischemia-reperfusion injury,II/R)和腸黏膜屏障完整性的影響。方法:采用夾閉大鼠腸系膜上動(dòng)脈(superior mesenteric artery,SMA)的方法建立腸缺血/再灌注損傷模型的Wistar大鼠40只。隨機(jī)分為4組:假手術(shù)組(Sham組)、II/R組、PRO(10mg/kg)組、DEX(5μg/kg)組,每組10只。藥物預(yù)處理組于夾閉SMA前20 min,分別經(jīng)股靜脈輸注PRO、DEX;于灌注末2 h抽腔靜脈血測(cè)血清二胺氧化酶(diamine oxidase,DAO)活性,用光鏡觀察腸黏膜上皮病變并行組織病理?yè)p傷評(píng)分(Chiu's評(píng)分)。結(jié)果:1、與Sham組比較,II/R組DAO活性明顯增高(P0.05);PRO預(yù)處理II/R可降低DAO的含量(34.29±7.33 vs 45.46±8.46;(P0.05);但較Sham組及DEX組升高((P0.05);DEX預(yù)處理II/R后能顯著降低DAO的含量且較PRO組更有意義,其含量為(23.33±7.06 vs 45.46±8.46;(P0.05),但較Sham組升高(P0.05)。2、II/R組腸絨毛破潰,腸黏膜變薄,其完整性破壞嚴(yán)重。PRO與DEX相比對(duì)II/R保護(hù)效應(yīng)較差,兩組各參數(shù)比較差異仍有統(tǒng)計(jì)學(xué)意義(P0.05);DEX預(yù)處理與II/R組相比,可使腸粘膜損傷降低,很好維護(hù)腸粘膜的完整性,且優(yōu)于PRO。結(jié)論:腸缺血/再灌注后,嚴(yán)重的破壞腸黏膜屏障完整性。右美托咪定較丙泊酚預(yù)處理能更有效維護(hù)腸黏膜的完整性,減少血清DAO的表達(dá),能對(duì)大鼠腸缺血/再灌注的腸黏膜起到保護(hù)作用,且比丙泊酚組對(duì)受損腸道的保護(hù)作用更突出。
[Abstract]:Aim: to compare the effects of dexmetomidine (dexmedetomidine,DEX) and propofol (propofol,PRO) preconditioning on intestinal ischemia / reperfusion injury (intestinal ischemia-reperfusion injury,II/R) and intestinal mucosal barrier integrity in rats. Methods: 40 Wistar rats with intestinal ischemia / reperfusion injury were established by clamping superior mesenteric artery (superior mesenteric artery,SMA). They were randomly divided into 4 groups: sham operation group (Sham group) and pro (10mg/kg) group (10 rats in each group). The serum diamine oxidase (diamine oxidase,DAO) activity was measured 20 min, before SMA clipping in the drug preconditioning group by intravenous infusion of PRO,DEX; through femoral vein at the end of 2 hours of perfusion. The intestinal mucosal epithelial lesions were observed with light microscope and the pathological injury score (Chiu's) was evaluated. Results compared with Sham group, the activity of DAO in group 鈪，

本文編號(hào)：2229536