【摘要】：目的觀察神經(jīng)營養(yǎng)因子3(NT3)-殼聚糖載體對大鼠運(yùn)動皮層損傷后的前肢行為學(xué)功能恢復(fù)的效果,并檢測其對損傷區(qū)及側(cè)腦室下區(qū)神經(jīng)干細(xì)胞(NSCs)的增殖和分化的影響。方法 65只Wistar大鼠分為正常對照組(n=7)、單純損傷組(n=29)和NT3-殼聚糖組(n=29)。制作大鼠運(yùn)動皮層吸除損傷性腦損傷模型。NT3-殼聚糖組于手術(shù)后立即植入NT3-殼聚糖載體,單純損傷組不給予任何治療措施。分別于術(shù)后3 d、7 d、14 d、1個月、2個月和3個月,應(yīng)用食物球抓取實驗觀察大鼠前肢功能恢復(fù)情況,應(yīng)用HE染色觀察損傷區(qū)的空腔體積,應(yīng)用免疫熒光染色評價NSCs的增殖和分化。結(jié)果 NT3-殼聚糖組右側(cè)前肢抓取成功率高于單純損傷組右側(cè)(F6.00,P≤0.05)。NT3-殼聚糖組空腔體積均顯著小于單純損傷組(F629.5,P0.001)。在NSCs分化實驗中,NT3-殼聚糖組各時間點損傷區(qū)Brd U細(xì)胞數(shù)均顯著高于單純損傷組(F171.43,P0.001)。在NSCs增殖實驗中,NT3-殼聚糖組Brd U陽性細(xì)胞數(shù)顯著高于正常對照組和單純損傷組(F155.06,P0.001),術(shù)后7 d損傷同側(cè)Dcx陽性細(xì)胞數(shù)顯著高于單純損傷組(F=62.367,P0.001),Brd U/Dcx雙陽性細(xì)胞數(shù)顯著高于正常對照組(F=33.527,P0.001)。結(jié)論 NT3-殼聚糖載體可增加腦損傷所致的側(cè)腦室下區(qū)NSCs的增殖,促進(jìn)損傷區(qū)新生神經(jīng)元的發(fā)生以及大鼠前肢運(yùn)動功能恢復(fù)。
[Abstract]:Objective to observe the effect of neurotrophic factor 3 (NT3)-chitosan carrier on the recovery of behavioral function of forelimb after motor cortex injury in rats, and to detect the effect of neurotrophic factor 3 (NT3)-chitosan carrier on the proliferation and differentiation of neural stem cells (NSCs) in injured and subventricular regions. Methods 65 Wistar rats were divided into normal control group (n = 7), simple injury group (n = 29) and NT3-chitosan group (n = 29). The rat motor cortex injury model. NT3-chitosan group was implanted with NT3-chitosan immediately after operation, and the simple injury group was not given any therapeutic measures. The functional recovery of forelimb was observed by food ball grabbing experiment on the 3rd day, 7th day, 14th day, 1 month, 2 month and 3 month after operation, respectively. The cavity volume of injured area was observed by HE staining, and the proliferation and differentiation of NSCs were evaluated by immunofluorescence staining. Results the success rate of grasping the right forelimb in NT3-chitosan group was higher than that in simple injury group (F 6.00 P 鈮，

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