【摘要】：目的通過3種不同方法制備大鼠脊髓損傷致繼發(fā)性腸道功能障礙的模型,探究各組脊髓損傷致繼發(fā)性腸道功能障礙模型的表現(xiàn)差別,為脊髓損傷繼發(fā)腸道功能障礙的相關(guān)研究提供穩(wěn)定的實驗動物模型復(fù)制方法和實驗基礎(chǔ)。方法將48只SD大鼠隨機分為打擊組(A組,n=12)、全橫斷組(B組,n=12)、鉗夾組(C組,n=12)、假手術(shù)組(D組,n=12)。于術(shù)后1 d、3d、7 d分別對各組進行BBB功能評分;腸肌電慢波測定;血清D-乳酸含量測定;HE染色觀察腸組織形態(tài)。結(jié)果術(shù)后1 d、3d、7 d,A、B、C組BBB評分、肌電慢波振幅均低于D組(P0.05);Chiu腸黏膜評分、血清D-乳酸濃度均顯著高于D組(P0.05)。術(shù)后第7天,A組血清D-乳酸濃度和Chiu腸黏膜評分與B、C兩組間差異存在顯著性(P0.05)。結(jié)論不同方法制備脊髓損傷后,各組均繼發(fā)腸道功能障礙,其中A組模型復(fù)制較為穩(wěn)定,且死亡率較低,更適用于周期較長的實驗研究。
[Abstract]:Objective to establish the model of secondary intestinal dysfunction induced by spinal cord injury in rats by three different methods, and to explore the difference of secondary intestinal dysfunction model caused by spinal cord injury in each group. To provide a stable experimental animal model replication method and experimental basis for the related research of secondary intestinal dysfunction after spinal cord injury. Methods Forty-eight Sprague-Dawley rats were randomly divided into three groups: group A (n = 12), group B (n = 12), group C (n = 12) and group D (n = 12). BBB score, slow wave measurement of intestinal electromyography, and serum D- lactic acid content were measured on the 7th day after operation, and the intestinal morphology was observed by HE staining. Results the BBB score and the amplitude of EMG slow wave in group C were lower than those in group D (P0.05), and the serum levels of D-lactic acid were significantly higher than those in group D (P0.05). On the 7th day after operation, there were significant differences in serum D- lactic acid concentration and Chiu intestinal mucosal score between group A and group C (P0.05). Conclusion after the spinal cord injury was prepared by different methods, all the groups had secondary intestinal dysfunction. The model of group A was more stable, and the mortality was lower, which was more suitable for the experimental study with a longer period.
【作者單位】： 成都醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)實驗教學(xué)中心;
【基金】：四川省教育廳2016年度科研計劃重點項目(16ZA0296) 成都醫(yī)學(xué)院實驗室開放項目(kf2016003)
【分類號】：R-332;R651.2

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