【摘要】：目的:本課題旨在探討音猬因子(Shh)聯(lián)合甲基強(qiáng)的松龍(MP)的治療方法,治療大鼠脊髓損傷的實驗研究。方法:本次研究選取40只雌性SD大鼠,體重均為200-250g。采用改良Allen打擊法制備大鼠急性脊髓損傷模型,蛛網(wǎng)膜下腔置管加尾靜脈注射兩者給藥方式。將實驗動物隨機(jī)分為4組,每組10只:空白對照A組采用造模后24h內(nèi)尾靜脈注入生理鹽水+蛛網(wǎng)膜下腔注入生理鹽水連續(xù)30天;單一治療B組采用造模后24h內(nèi)尾靜脈注入MP+蛛網(wǎng)膜下腔注入生理鹽水連續(xù)30天;單一治療C組采用造模后24h內(nèi)尾靜脈注入生理鹽水+蛛網(wǎng)膜下腔注入shh連續(xù)30天;聯(lián)合治療D組采用造模后24h內(nèi)尾靜脈注入MP+蛛網(wǎng)膜下腔注入shh連續(xù)30天。在造模成功后每周均對各組實驗動物行BBB評分,直至造模成功后第五周以評價神經(jīng)運(yùn)動功能恢復(fù)情況;在BBB評分結(jié)束后處死各組實驗動物,取材行HE染色比較兩組大鼠脊髓損傷后病理變化。結(jié)果:BBB評分:聯(lián)合治療組BBB評分明顯高于其他實驗三組,且差異具有統(tǒng)計學(xué)意義(P0.05);MP組BBB評分高于空白對照組以及Shh組,差異具有統(tǒng)計學(xué)意義(P0.05);Shh組較空白對照組BBB評分改變不明顯,差異不具有統(tǒng)計學(xué)意義(P0.05)。HE染色顯示聯(lián)合治療組較其他實驗三組脊髓損傷后空洞面積明顯縮小,且差異具有統(tǒng)計學(xué)意義(P0.05);MP組脊髓損傷后空洞面積小于空白對照組以及Shh組,差異具有統(tǒng)計學(xué)意義(P0.05);Shh組較空白對照組脊髓損傷后空洞面積縮小不明顯,差異不具有統(tǒng)計學(xué)意義(P0.05)。結(jié)論:1.音猬因子單獨(dú)使用對大鼠脊髓損傷后功能的恢復(fù)作用不明顯,但甲基強(qiáng)的松龍單獨(dú)使用可促進(jìn)大鼠脊髓損傷后功能的恢復(fù);2.音猬因子聯(lián)合甲基強(qiáng)的松龍治療可以明顯改善脊髓損傷后的功能恢復(fù)。
[Abstract]:Aim: to investigate the therapeutic effect of (Shh) combined with methylprednisolone (MP) on spinal cord injury (sci) in rats. Methods: 40 female Sprague-Dawley rats (200-250 g) were selected in this study. The model of acute spinal cord injury in rats was established by modified Allen method. The two drugs were administered by subarachnoid tube and caudal vein injection. The experimental animals were randomly divided into 4 groups with 10 rats in each group: control group A was injected with saline subarachnoid intravenously for 30 days within 24 hours after modeling. In group B, saline was injected into subarachnoid cavity of MP within 24 hours after modeling, and shh was injected into subarachnoid space of tail vein in group C for 30 days. Group D was treated with injection of shh into subarachnoid cavity of MP within 24 hours after modeling. Every week after the success of the model, the experimental animals were evaluated with BBB score until the fifth week after the model was made, and the experimental animals were killed after the BBB score was finished. The pathological changes after spinal cord injury in two groups were compared by HE staining. Results: the BBB score of the combined treatment group was significantly higher than that of the other three experimental groups, and the difference was statistically significant (P0.05) the BBB score of MP group was higher than that of the blank control group and Shh group. The difference was statistically significant (P0.05) the BBB score in the Shh group was not significantly different from that in the blank control group (P0.05). The HE staining showed that the cavity area in the combined treatment group was significantly smaller than that in the other three experimental groups. The difference was statistically significant (P0.05). The area of cavities after spinal cord injury in MP group was smaller than that in control group and Shh group. The difference was statistically significant (P0.05). Conclusion 1. The effect of Hedgehog on the recovery of function after spinal cord injury in rats was not obvious, but methylprednisolone alone could promote the recovery of function after spinal cord injury in rats. Hedgehog factor combined with methylprednisolone can significantly improve the functional recovery after spinal cord injury.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R651.2

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