【摘要】：目的評價右美托咪定對脾切除幼鼠外周血T淋巴細胞增殖及T細胞亞群的影響。方法健康雄性SD大鼠24只,6周齡,體重130~150g,隨機分為四組:生理鹽水組(S組)、右美托咪定組(D組)、生理鹽水組+脾切除(SN組)和右美托咪定組+脾切除(SD組),每組6只。S、D組不做外科處理,SN、SD組制備脾切除模型。喂養(yǎng)1周后S組和SN組腹腔注射生理鹽水10ml/kg,D組和SD組腹腔注射右美托咪定50μg/kg。給藥后2h,取血樣,采用MTT法檢測外周血T淋巴細胞增殖能力,采用流式細胞術檢測T細胞亞群即CD4~+、CD8~+細胞含量,計算CD4~+/CD8~+值。結(jié)果與S組比較,SN組T淋巴細胞增殖能力降低,CD4~+、CD8~+含量及CD4~+/CD8~+值降低,D組T淋巴細胞增殖能力明顯降低(P0.05);與D組比較,SD組T淋巴細胞增殖能力明顯降低,CD4~+、CD8~+含量及CD4~+/CD8~+值明顯降低(P0.05);與SN組比較,SD組T淋巴細胞增殖能力明顯降低(P0.05)。結(jié)論右美托咪定對正常幼鼠和免疫低下幼鼠細胞免疫均有一定抑制作用,且對免疫低下幼鼠的抑制作用更強。
[Abstract]:Objective to evaluate the effect of dexmetomidine on T lymphocyte proliferation and T cell subsets in peripheral blood of splenectomy young rats. Methods 24 male Sprague-Dawley rats were aged 6 weeks. The rats were randomly divided into four groups: normal saline group (S group), dexmetomidine group (D group), splenectomy group (SN group) and desmetomidine group (SD group). One week after feeding, normal saline 10 ml / kg D and SD group were intraperitoneally injected with dexmetomidine 50 渭 g / kg / kg in S group and SN group. Two hours after administration, blood samples were collected and the proliferation ability of peripheral blood T lymphocytes was detected by MTT assay. The T cell subsets (CD4 ~ / CD8 ~ cells) were measured by flow cytometry, and the CD4 ~ / CD8~ value was calculated. Results compared with S group, the proliferation ability of T lymphocytes in S group decreased the content of CD4 ~ + CD8 ~ and CD4 ~ / CD8 ~ value of T lymphocyte in D group (P0.05), and the proliferation ability of T lymphocyte in SD group was significantly lower than that in D group (P0.05), and that in SD group was significantly lower than that in D group (P0.05). Compared with SN group, the proliferative ability of T lymphocytes in SD group was significantly lower than that in SN group (P0.05). Conclusion dexmetomidine has a certain inhibitory effect on the cellular immunity of both normal and immunodeficient young rats, and has a stronger inhibitory effect on the immunosuppression of young rats.
【作者單位】： 蘭州大學第一醫(yī)院麻醉科;
【分類號】：R614

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