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  本文選題：A型肉毒桿菌毒素 + 加巴噴丁�。� 參考：《首都醫(yī)科大學學報》2017年03期

【摘要】：目的評價A型肉毒素復合低劑量加巴噴丁對切口痛大鼠行為學及脊髓背角神經激肽-1(neurokinin-1,NK-1)受體內化的影響。方法雄性SD大鼠,體質量280~300 g,6~8周齡。采用數(shù)字表法隨機分為5組(n=9):對照組(Control組)、切口痛—鹽水組(Saline組)、切口痛—加巴噴丁組(GBP組)、切口痛—毒素組(Bo NT/A組)、切口痛—毒素復合加巴噴丁組(G+B組)。Bo NT/A組和G+B組于造模前1 d鞘內注射Bo NT/A 0.5U,GBP組和G+B組于造模前30 min鞘內注射GBP 50 mg。切口痛模型制備后3 h,每組各隨機抽取6只進行累計疼痛評分(cumulative pain scores,CPS)和機械縮足閾值(paw withdrawal threshold,PWT)的測量;每組各抽取3只,通過免疫熒光技術測定NK-1受體內化情況。結果與Control組比較,切口后3 h Saline組、GBP組、Bo NT/A組和G+B組右后足CPS升高、PWT降低、脊髓背角NK-1受體內化神經元數(shù)目上調(P0.05);與Saline組比較,Bo NT/A組和G+B組CPS降低、PWT升高、脊髓背角NK-1受體內化神經元數(shù)目下調(P0.05),GBP組差異無統(tǒng)計學意義(P0.05);與Bo NT/A組比較,G+B組CPS降低、PWT升高、脊髓背角NK-1受體內化神經元數(shù)目下調(P0.05)。結論單獨應用低劑量GBP對大鼠切口痛無效,Bo NT/A復合低劑量GBP治療切口痛大鼠的術后疼痛有明顯的協(xié)同作用,療效優(yōu)于單獨應用Bo NT/A,其鎮(zhèn)痛機制可能與抑制切口痛大鼠脊髓背角NK-1受體內化有關。
[Abstract]:Objective to evaluate the effects of botulinum toxin A (Botulinum A) combined with low dose of gabapentin on the behavior and the internalization of neurokinin-1 (NK-1) receptor of spinal dorsal horn neurokinin-1 in rats with incision pain. Methods male Sprague-Dawley rats with body mass of 280 ~ 300 g / L and 8 weeks of age were used. Five groups were randomly divided into 5 groups by digital table: control group, saline group, incision pain plus gabapentin group GBP group, incision pain toxin group Bo NT / A group, incision pain toxin combined with Bapentin group, incision pain toxin combined with Bappentin group, G group. Bo NT / A group. Group G B and group G B were injected intrathecal injection of Bo NT / A 0.5 U GBP 1 d before model making, and group G B injected 50 mg of GBP 30 min before modeling. At 3 hours after the establishment of incision pain model, 6 rats in each group were randomly selected for measurement of cumulative pain score (pain score) and mechanical foot contraction threshold (paw withdrawal thresholdpain), and 3 rats in each group were selected for the determination of NK-1 receptor internalization by immunofluorescence technique. Results compared with the control group, the CPS of the right hind foot in the Saline group increased and the number of NK-1 receptor internalized neurons in the dorsal horn of spinal cord increased, compared with the Saline group, the CPS of the Bo NTA group and the G B group decreased the PWT increase, compared with the Saline group, the CPS of the Bo NT / A group and G B group decreased the PWT level. There was no significant difference in the number of NK-1 receptor internalized neurons in the dorsal horn of spinal cord (P 0.05), but the number of CPS in group G B was lower than that in group B, and the number of neurons internalized in NK-1 receptor in the dorsal horn of spinal cord was down-regulated (P 0.05). Conclusion low dose GBP alone has a synergistic effect on postoperative pain in rats with incision pain. The analgesic mechanism may be related to the inhibition of NK-1 receptor internalization in the spinal dorsal horn of rats with incision pain.
【作者單位】： 首都醫(yī)科大學附屬北京朝陽醫(yī)院麻醉科;
【基金】：國家自然科學基金(81571065,81428008,81400909)~~
【分類號】：R614

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