本文選題：溫度敏感性離子通道TRPV3 + 軟骨�。� 參考：《山西醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的:檢測溫度敏感性離子通道TRPV3(thermosensitive ion channnel TRPV3)在人的膝關(guān)節(jié)滑膜及軟骨標(biāo)本中的分布,對人滑膜及軟骨組織中TRPV3所起的作用進(jìn)行研究。方法:集取各種年齡人正常的膝關(guān)節(jié)滑膜及軟骨分別10例和5例,膝骨關(guān)節(jié)炎軟骨及滑膜組織各5例,每例軟骨組織均取自脛骨平臺,滑膜組織標(biāo)本均取自關(guān)節(jié)囊內(nèi)層,分裝在-70℃冰箱中。番紅“O”染色證實(shí)軟骨標(biāo)本有無退行性變。首先分出5例OA軟骨標(biāo)本為OA組,取5例60-69歲為正常對照組,運(yùn)用Western blot、RT-PCR檢測正常和OA軟骨、滑膜中TRPV3有無表達(dá)分布及趨勢,再用免疫熒光和免疫組化方法測得TRPV3在正常及OA軟骨中分布表達(dá)以及趨勢。然后Western blot檢測正常軟骨標(biāo)本,所得條帶測灰度值,分30-39歲組和60-69歲組各5例,最后采取Western blot檢測TRPV3位于兩組軟骨中的趨勢。結(jié)果:番紅“O”染色OA軟骨標(biāo)本顯微鏡觀察異常,色度及分層模糊,細(xì)胞之間著色較低。正常軟骨標(biāo)本染色均勻,淺層到深層染色由淺至深,顯示無退行性改變;RT-PCR、Western blot、免疫熒光與免疫組化方法在軟骨及滑膜組織中都有TRPV3表達(dá),免疫熒光檢測TRPV3在軟骨細(xì)胞膜上有表達(dá),且OA較正常軟骨表達(dá)較強(qiáng);考馬斯亮藍(lán)染色控制上樣量,檢測TRPV3條帶灰度值分析,結(jié)果顯示TRPV3的表達(dá)量OA組高于正常對照組,t檢驗(yàn)有統(tǒng)計(jì)意義(P0.05)。測得兩個年齡組正常軟骨標(biāo)本TRPV3的表達(dá)量,結(jié)果表明TRPV3的表達(dá)隨年齡的增加而升高,t檢驗(yàn)有統(tǒng)計(jì)意義(p0.05)。結(jié)論:TRPV3與骨關(guān)節(jié)炎有著密切的聯(lián)系,在骨關(guān)節(jié)炎的發(fā)生發(fā)展當(dāng)中起一定作用,為關(guān)節(jié)軟骨損傷的防治提供新靶點(diǎn)。
[Abstract]:Objective: to detect the distribution of temperature sensitive ion channel TRPV3 (thermosensitive ion channnel TRPV3) in human knee joint synovium and cartilage specimens, and to study the role of TRPV3 in human synovium and cartilage tissue. Methods: 10 cases of normal knee joint and 5 cases of articular cartilage of the knee and osteoarthritis cartilage of all ages were collected. And 5 cases of synovial tissue, each case of cartilage tissue was taken from the tibial plateau, and the synovial tissue specimens were taken from the inner layer of the joint capsule. It was installed in the -70 C fridge. "O" staining showed no degeneration of cartilage specimens. First, 5 cases of OA cartilage specimens were divided into group OA, 5 cases were 60-69 years old as normal control group, and Western blot, RT-PCR was used to detect normal. The expression distribution and trend of TRPV3 in OA cartilage and synovial membrane were observed, and the distribution and trend of TRPV3 in normal and OA cartilage were measured by immunofluorescence and immunohistochemistry. Then Western blot was used to detect normal cartilage specimens, and the gray value of the strip was measured, and 5 cases were divided into 30-39 years old group and 60-69 year old group. Finally, Western blot was used to detect TRPV3. The trend in the two groups of cartilage. Results: the "O" stained OA cartilage specimens were observed by microscope, the chromaticity and stratification were blurred, the coloring between cells was low. The normal cartilage specimens were dyed evenly, the superficial to deep deep staining was from shallow to deep, showing no degenerative changes; RT-PCR, Western blot, immunofluorescence and immunohistochemical methods in cartilage and synovial groups TRPV3 expression was found in all the tissues, and the expression of TRPV3 on the chondrocyte membrane was expressed by immunofluorescence, and the expression of OA was stronger than that of the normal cartilage. Coomassie brilliant blue staining was used to control the sample quantity and to detect the gray value of the TRPV3 strip. The results showed that the OA group of TRPV3 was higher than the normal control group, and the t examination was statistically significant (P0.05). The two age groups were normal soft. The expression of TRPV3 in bone specimen shows that the expression of TRPV3 increases with the increase of age and t test has statistical significance (P0.05). Conclusion: TRPV3 has a close relationship with osteoarthritis, which plays a role in the development of osteoarthritis and provides new targets for the prevention and treatment of articular cartilage injury.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R684.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 馬悅穎;李滄海;霍海如;姜廷良;;瞬時感受器電位V亞家族離子通道——溫度感受器[J];醫(yī)學(xué)分子生物學(xué)雜志;2007年02期

2 ;Ion channels in neuronal survival[J];Science China(Life Sciences);2010年03期

3 邢東明,張平,王琦,韓鳳岳,羅致誠;壓應(yīng)力對體外培養(yǎng)的軟骨細(xì)胞產(chǎn)生細(xì)胞因子的影響[J];解剖學(xué)報;2001年04期

4 史娟,李繼碩;TRP離子通道[J];神經(jīng)解剖學(xué)雜志;2004年02期

5 楊宇,曹宇;溫度感受相關(guān)的TRP蛋白家族研究進(jìn)展[J];生理科學(xué)進(jìn)展;2005年01期

，

本文編號：2021633