本文選題：磷酸鈣類 + 膝關節(jié)��； 參考：《中國組織工程研究》2017年02期

【摘要】：背景:理想的骨軟骨組織工程支架應巧妙地模擬人體正常關節(jié)骨軟骨結(jié)構(gòu)。目的:基于關節(jié)骨軟骨的解剖結(jié)構(gòu)、生理功能,制備致密層骨軟骨復合支架,同時觀察其修復兔關節(jié)骨軟骨缺損的效果。方法:利用快速成型技術(shù)在三維立體包芯結(jié)構(gòu)骨架表面噴涂乳酸-羥基乙酸共聚物/β-磷酸三鈣有機溶液,形成0.5 mm的致密層;利用"溶解-粘連"工藝能將定向微管結(jié)構(gòu)軟骨支架與致密層相互連接,形成致密層骨軟骨復合支架。取60只家兔,制備左膝關節(jié)軟骨全層缺損,隨機分3組,實驗組植入致密層骨軟骨復合支架,對照組植入定向微管結(jié)構(gòu)軟骨支架,空白對照組不植入任何材料,修復后12,24周進行缺損部位大體與組織學觀察。結(jié)果與結(jié)論:1大體觀察結(jié)果:對照組修復后12周存在明顯缺損部位,未見明顯修復痕跡;24周缺損面積減少,可見新生組織覆蓋,但表面粗糙。實驗組修復12周后缺損部位表面平整,質(zhì)地較軟,與周圍組織邊界不清;修復后24周,被透明軟骨樣組織覆蓋,表面平整�？瞻讓φ战M修復效果較差;2組織學觀察結(jié)果:修復后12周,對照組缺損部位出現(xiàn)形態(tài)不規(guī)則的骨痂,但未形成骨小梁;實驗組出現(xiàn)新生骨,軟骨厚度與正常軟骨接近,并且軟骨下存在不規(guī)則骨小梁。修復后24周,對照組出現(xiàn)新生軟骨,但厚度不均,高低不平,骨小梁結(jié)構(gòu)不規(guī)則;實驗組組織與正常組織無明顯差異,軟骨表面光滑;空白對照組修復效果較差;3結(jié)果表明:致密層骨軟骨復合支架接近人體正常關節(jié)骨軟骨結(jié)構(gòu),可促進關節(jié)軟骨缺損的修復。
[Abstract]:Background: the ideal scaffold for osteochondral tissue engineering should mimic the normal articular cartilage structure. Aim: based on the anatomical structure and physiological function of articular cartilage, the composite scaffolds of dense lamellar cartilage were prepared and the effect of repairing articular cartilage defects in rabbits was observed at the same time. Methods: rapid prototyping technique was used to spray lactic acid-glycolic acid copolymer / 尾 -tricalcium phosphate organic solution on the surface of three-dimensional core structure skeleton to form a dense layer of 0.5 mm. The composite scaffold of dense layer bone cartilage can be formed by connecting the oriented microtubule cartilage scaffold with the dense layer by the "dissolving-adhesion" process. Sixty rabbits were randomly divided into 3 groups: the experimental group was implanted with compact lamellar cartilage composite scaffold, the control group was implanted with directional microtubule cartilage scaffold, and the blank control group was not implanted with any material. Gross and histological observations of the defect were made 24 weeks after repair. Results and conclusion: in the control group, there were obvious defects at 12 weeks after repair, and no obvious signs of restoration were found in the control group. The defect area was decreased after 24 weeks, but the surface was rough. After 12 weeks of repair, the defect in the experimental group was smooth and soft, and the boundary with the surrounding tissue was not clear. 24 weeks after repair, the defect was covered with transparent cartilage tissue and the surface was smooth. The results of histological observation showed that the defect site of control group had irregular shape callus but no trabecular bone at 12 weeks after repair, and the new bone appeared in experimental group, and the thickness of cartilage was close to that of normal cartilage. And there are irregular trabeculae under cartilage. 24 weeks after repair, new cartilage appeared in the control group, but the thickness was uneven, bone trabecular structure was irregular, there was no significant difference between the experimental group and normal tissue, and the cartilage surface was smooth. The results of the blank control group showed that the compact lamellar cartilage composite scaffold was close to the normal articular cartilage structure and could promote the repair of articular cartilage defect.
【作者單位】： 鄭州大學附屬洛陽中心醫(yī)院;河南大學第一臨床學院;
【基金】：2016年洛陽市科技計劃醫(yī)療衛(wèi)生項目(1603002A-15) 河南省重點實驗室研究項目(J01501153)~~
【分類號】：R318.08;R684

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8 高曉s，

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