本文選題：磷酸鈣類 + 膝關(guān)節(jié)�。� 參考：《中國組織工程研究》2017年02期

【摘要】：背景:理想的骨軟骨組織工程支架應(yīng)巧妙地模擬人體正常關(guān)節(jié)骨軟骨結(jié)構(gòu)。目的:基于關(guān)節(jié)骨軟骨的解剖結(jié)構(gòu)、生理功能,制備致密層骨軟骨復(fù)合支架,同時觀察其修復(fù)兔關(guān)節(jié)骨軟骨缺損的效果。方法:利用快速成型技術(shù)在三維立體包芯結(jié)構(gòu)骨架表面噴涂乳酸-羥基乙酸共聚物/β-磷酸三鈣有機溶液,形成0.5 mm的致密層;利用"溶解-粘連"工藝能將定向微管結(jié)構(gòu)軟骨支架與致密層相互連接,形成致密層骨軟骨復(fù)合支架。取60只家兔,制備左膝關(guān)節(jié)軟骨全層缺損,隨機分3組,實驗組植入致密層骨軟骨復(fù)合支架,對照組植入定向微管結(jié)構(gòu)軟骨支架,空白對照組不植入任何材料,修復(fù)后12,24周進行缺損部位大體與組織學(xué)觀察。結(jié)果與結(jié)論:1大體觀察結(jié)果:對照組修復(fù)后12周存在明顯缺損部位,未見明顯修復(fù)痕跡;24周缺損面積減少,可見新生組織覆蓋,但表面粗糙。實驗組修復(fù)12周后缺損部位表面平整,質(zhì)地較軟,與周圍組織邊界不清;修復(fù)后24周,被透明軟骨樣組織覆蓋,表面平整�？瞻讓φ战M修復(fù)效果較差;2組織學(xué)觀察結(jié)果:修復(fù)后12周,對照組缺損部位出現(xiàn)形態(tài)不規(guī)則的骨痂,但未形成骨小梁;實驗組出現(xiàn)新生骨,軟骨厚度與正常軟骨接近,并且軟骨下存在不規(guī)則骨小梁。修復(fù)后24周,對照組出現(xiàn)新生軟骨,但厚度不均,高低不平,骨小梁結(jié)構(gòu)不規(guī)則;實驗組組織與正常組織無明顯差異,軟骨表面光滑;空白對照組修復(fù)效果較差;3結(jié)果表明:致密層骨軟骨復(fù)合支架接近人體正常關(guān)節(jié)骨軟骨結(jié)構(gòu),可促進關(guān)節(jié)軟骨缺損的修復(fù)。
[Abstract]:Background: the ideal scaffold for osteochondral tissue engineering should mimic the normal articular cartilage structure. Aim: based on the anatomical structure and physiological function of articular cartilage, the composite scaffolds of dense lamellar cartilage were prepared and the effect of repairing articular cartilage defects in rabbits was observed at the same time. Methods: rapid prototyping technique was used to spray lactic acid-glycolic acid copolymer / 尾 -tricalcium phosphate organic solution on the surface of three-dimensional core structure skeleton to form a dense layer of 0.5 mm. The composite scaffold of dense layer bone cartilage can be formed by connecting the oriented microtubule cartilage scaffold with the dense layer by the "dissolving-adhesion" process. Sixty rabbits were randomly divided into 3 groups: the experimental group was implanted with compact lamellar cartilage composite scaffold, the control group was implanted with directional microtubule cartilage scaffold, and the blank control group was not implanted with any material. Gross and histological observations of the defect were made 24 weeks after repair. Results and conclusion: in the control group, there were obvious defects at 12 weeks after repair, and no obvious signs of restoration were found in the control group. The defect area was decreased after 24 weeks, but the surface was rough. After 12 weeks of repair, the defect in the experimental group was smooth and soft, and the boundary with the surrounding tissue was not clear. 24 weeks after repair, the defect was covered with transparent cartilage tissue and the surface was smooth. The results of histological observation showed that the defect site of control group had irregular shape callus but no trabecular bone at 12 weeks after repair, and the new bone appeared in experimental group, and the thickness of cartilage was close to that of normal cartilage. And there are irregular trabeculae under cartilage. 24 weeks after repair, new cartilage appeared in the control group, but the thickness was uneven, bone trabecular structure was irregular, there was no significant difference between the experimental group and normal tissue, and the cartilage surface was smooth. The results of the blank control group showed that the compact lamellar cartilage composite scaffold was close to the normal articular cartilage structure and could promote the repair of articular cartilage defect.
【作者單位】： 鄭州大學(xué)附屬洛陽中心醫(yī)院;河南大學(xué)第一臨床學(xué)院;
【基金】：2016年洛陽市科技計劃醫(yī)療衛(wèi)生項目(1603002A-15) 河南省重點實驗室研究項目(J01501153)~~
【分類號】：R318.08;R684

【參考文獻】

相關(guān)期刊論文 前9條

1 王虔;馬云勝;李德華;;不同來源蠶絲蛋白修復(fù)骨軟骨組織缺損的效果比較[J];中國組織工程研究;2015年52期

2 張海峰;杜子婧;姜聞博;韓冬;;3D打印PLA-HA復(fù)合材料與骨髓基質(zhì)細胞的相容性研究[J];組織工程與重建外科雜志;2015年06期

3 薛震;牛麗媛;安剛;郭亞山;呂松岑;;可注射式納米羥基磷灰石復(fù)合支架與骨髓間充質(zhì)干細胞修復(fù)兔橈骨骨缺損[J];中國組織工程研究;2015年03期

4 陳加榮;張余;黃華揚;夏虹;尹慶水;;磁力靶向傳遞SPIO標記的BMSC修復(fù)關(guān)節(jié)軟骨缺損的研究進展[J];中國骨科臨床與基礎(chǔ)研究雜志;2014年02期

5 劉拴;楊洪平;張衛(wèi)國;;關(guān)節(jié)鏡下關(guān)節(jié)清理術(shù)治療膝關(guān)節(jié)骨性關(guān)節(jié)炎的療效分析[J];實用臨床醫(yī)藥雜志;2014年01期

6 劉奕;謝林;;組織工程技術(shù)修復(fù)損傷關(guān)節(jié)軟骨的研究與應(yīng)用[J];中國組織工程研究;2013年41期

7 魏波;金成哲;徐燕;唐成;胡文浩;王黎明;;BMSCs來源細胞外基質(zhì)支架對微骨折骨髓刺激術(shù)后血凝塊體外軟骨化分化的作用研究[J];中國修復(fù)重建外科雜志;2013年04期

8 高曉s，

本文編號：2003903