本文選題：回腸貯袋炎 + 腸屏障��； 參考：《天津醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的:建立大鼠回腸貯袋炎模型,研究大鼠回腸貯袋炎的腸屏障變化,并探討其發(fā)生改變的機(jī)制。方法:利用顯微外科技術(shù)建立大鼠回腸貯袋模型,將模型大鼠隨機(jī)分為IPAA組(接受回腸貯袋手術(shù),術(shù)后正常飲水)和DSS組(接受回腸貯袋手術(shù),術(shù)后第30天始,自由飲用5%DSS溶液4天),并設(shè)假手術(shù)組(接受開關(guān)腹手術(shù),術(shù)后正常飲水)作為對照。實(shí)驗(yàn)期間觀察記錄各組大鼠體重變化、便血情況、糞便評分;術(shù)后第35天,處死全部大鼠,假手術(shù)組取末端回腸組織,IPAA組及DSS組取貯袋組織及貯袋近端回腸組織,HE染色進(jìn)行組織學(xué)評分;免疫組化染色檢測組織MPO、occludin蛋白表達(dá)水平;留取血標(biāo)本,采用比色法測定血D-乳酸濃度;實(shí)時(shí)熒光定量PCR檢測組織IL-1β、IL-6、TNF-α和IL-10的m RNA轉(zhuǎn)錄水平。結(jié)果:各組大鼠術(shù)后體重在短期下降之后逐漸增長,術(shù)后第30-35天,DSS組體重再次持續(xù)下降,假手術(shù)組和IPAA組則繼續(xù)增長;DSS組大鼠均出現(xiàn)血便(6/6),假手術(shù)組(0/6)和IPAA組(0/6)均無便血;DSS組糞便評分低于假手術(shù)組(P0.01)和IPAA組(P0.01),IPAA組糞便評分低于假手術(shù)組(P0.01);DSS組和IPAA組貯袋的組織學(xué)評分均高于DSS組回腸、IPAA組回腸和假手術(shù)組回腸(P0.01),且DSS組貯袋高于IPAA組貯袋(P0.01),而各組回腸的組織學(xué)評分之間差異無統(tǒng)計(jì)學(xué)意義(P0.05)。DSS組和IPAA組的MPO蛋白表達(dá)水平,血D-乳酸濃度,IL-1β、IL-6和TNF-α的m RNA轉(zhuǎn)錄水平均高于假手術(shù)組(P0.01),且DSS組均高于IPAA組(P0.01)。DSS組和IPAA組的occludin蛋白表達(dá)水平和IL-10m RNA轉(zhuǎn)錄水平均低于假手術(shù)組(P0.01),且DSS組均低于IPAA組(P0.01)。結(jié)論:1.成功建立大鼠回腸貯袋模型,并使用DSS成功誘導(dǎo)大鼠回腸貯袋炎模型。2.大鼠回腸貯袋炎模型的腸屏障結(jié)構(gòu)和功能受損,受損程度和炎癥程度呈正相關(guān)。3.促炎因子相對增多和抗炎因子相對減少促進(jìn)腸屏障受損。4.針對促炎因子、抗炎因子進(jìn)行的治療,可能對腸屏障結(jié)構(gòu)和功能的恢復(fù)有效。
[Abstract]:Aim: to establish a rat model of ileal pouch inflammation, to study the changes of intestinal barrier in rats with ileal pouch inflammation, and to explore the mechanism of the changes. Methods: rat ileal bag model was established by microsurgical technique. The model rats were randomly divided into two groups: IPAA group (receiving ileal bag operation, normal drinking water after operation) and DSS group (receiving ileal bag operation 30 days after operation). Free drinking of DSS solution for 4 days, and sham operation group (operation on switch abdomen, normal drinking water after operation) as control group. During the experiment, the changes of body weight, hematochezia and stool score were observed and recorded, and all the rats were killed on the 35th day after operation. In the sham operation group, the terminal ileum tissue of IPAA group and the DSS group were taken from the bag tissue and the proximal ileum tissue for histological grading; the expression of MPOOOOcludin protein was detected by immunohistochemical staining; the blood samples were collected. The serum Dlactic acid concentration was measured by colorimetry, and the mRNA transcription levels of IL-6TNF- 偽 and IL-10 were detected by real-time fluorescence quantitative PCR. Results: the weight of the rats in each group increased gradually after the short-term decrease, and the weight of the DSS group decreased again on the 30th to 35th day after operation. In the sham operation group and the IPAA group, the stool score in the DSS group was lower than that in the sham operation group and the IPAA group. The stool score in the DSS group was lower than that in the sham operation group (P 0.01) and the stool score in the IPAA group was lower than that in the sham operation group (P 0.01) and in the IPAA group (P 0.01 / 6). The stool score in the DSS group was lower than that in the sham operation group (P 0.01) and the stool score in the IPAA group was lower than that in the IPAA group. The histological scores of DSS group were higher than that of DSS group IPAA group and sham operation group (P 0.01), and that of DSS group was higher than that of IPAA group (P 0.01), but there was no significant difference in histological score between DSS group and IPAA group (P 0.05) and IPAA group (P < 0.05), the expression level of MPO protein in DSS group was higher than that in IPAA group, and the expression of MPO protein in DSS group was higher than that in IPAA group. The mRNA transcription levels of IL-1 尾 and TNF- 偽 in serum DLA were higher than those in sham operation group (P 0.01), and the levels of occludin protein expression and IL-10 mRNA transcription in DSS group were higher than those in IPAA group (P 0.01) and IPAA group (P 0.01), and the levels of IL-10 mRNA transcription in DSS group were all lower than those in IPAA group (P 0.01). Conclusion 1. The rat ileal bag model was successfully established and the rat ileal pouch model was successfully induced by DSS. The intestinal barrier structure and function of rat ileal pouch model were damaged, and the degree of injury was positively correlated with the degree of inflammation. The relative increase of proinflammatory factor and the decrease of anti-inflammatory factor promote intestinal barrier damage. 4. The treatment of proinflammatory factor and anti-inflammatory factor may be effective for the recovery of intestinal barrier structure and function.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R656.9

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王潔;何桂珍;王玉康;;腸道屏障功能測定方法的相關(guān)性[J];中華臨床營養(yǎng)雜志;2014年04期

2 楊韶華;周佾龍;衛(wèi)雅麗;朱龍金;;重癥急性胰腺炎患者腸屏障功能障礙與血流感染之間的關(guān)系研究[J];中華醫(yī)院感染學(xué)雜志;2014年14期

3 傅傳剛;高顯華;;直腸手術(shù)各種儲(chǔ)袋的應(yīng)用與評價(jià)[J];中華胃腸外科雜志;2014年05期

4 王印華;王寶華;唐明貴;白靜;浦踐一;;創(chuàng)傷后腸屏障功能障礙與免疫失調(diào)的相關(guān)性研究[J];腸外與腸內(nèi)營養(yǎng);2014年03期

5 董妮珊;鄭曙云;;腸屏障功能檢測方法的研究現(xiàn)狀及展望[J];中華老年多器官疾病雜志;2014年04期

6 李婷冶;陳潔;;腸屏障受損與2型糖尿病關(guān)系的研究進(jìn)展[J];醫(yī)學(xué)綜述;2013年21期

7 曾志宇;蘇藝群;;急性胰腺炎腸屏障功能障礙的研究現(xiàn)狀[J];醫(yī)學(xué)綜述;2013年20期

8 Orit Ianco;Hagit Tulchinsky;Michal Lusthaus;Amos Ofer;Erwin Santo;Nachum Vaisman;Iris Dotan;;Diet of patients after pouch surgery may affect pouch inflammation[J];World Journal of Gastroenterology;2013年38期

9 Maddalena Zippi;Claudio Cassieri;Eleonora Veronica Avallone;Roberta Pica;;Infliximab for the treatment of pouchitis[J];World Journal of Clinical Cases;2013年06期

10 宋希福;;重癥急性胰腺炎伴腸屏障功能障礙患者的腹內(nèi)壓變化及意義[J];中國現(xiàn)代普通外科進(jìn)展;2013年03期

，

本文編號：1960468