本文選題：脊髓 + 神經(jīng)干細(xì)胞��； 參考：《中國(guó)組織工程研究》2017年33期

【摘要】：背景:研究LINGO-1在脊髓神經(jīng)干細(xì)胞分化過(guò)程中的表達(dá)及效應(yīng)對(duì)調(diào)控神經(jīng)干細(xì)胞分化及修復(fù)脊髓損傷有著重要的意義。目的:觀察LINGO-1在脊髓神經(jīng)干細(xì)胞分化過(guò)程中的表達(dá)特征及生物學(xué)作用。方法:體外培養(yǎng)大鼠脊髓神經(jīng)干細(xì)胞,并對(duì)培養(yǎng)細(xì)胞進(jìn)行Nestin染色干性鑒定及誘導(dǎo)分化多能性鑒定。于分化第0天及第5天進(jìn)行LINGO-1與Nestin(神經(jīng)干細(xì)胞)、β-TubulinⅢ(神經(jīng)元細(xì)胞)、GFAP(星形膠質(zhì)細(xì)胞)及O4(少突膠質(zhì)細(xì)胞)免疫熒光雙染色,觀察LINGO-1的表達(dá)特征。體外培養(yǎng)的大鼠脊髓神經(jīng)干細(xì)胞分為對(duì)照組及干擾組,干擾組脊髓神經(jīng)干細(xì)胞感染LINGO-1 sh RNA慢病毒下調(diào)LINGO-1表達(dá),對(duì)照組脊髓神經(jīng)干細(xì)胞感染Scramble-sh RNA慢病毒,于感染第5天免疫熒光染色檢測(cè)神經(jīng)元突起長(zhǎng)度。結(jié)果與結(jié)論:(1)脊髓神經(jīng)干細(xì)胞可以分化成為神經(jīng)元、星形膠質(zhì)細(xì)胞和少突膠質(zhì)細(xì)胞;(2)LINGO-1在脊髓神經(jīng)干細(xì)胞及其分化的神經(jīng)元、少突膠質(zhì)細(xì)胞中表達(dá),但不在星形膠質(zhì)細(xì)胞中表達(dá);(3)下調(diào)LINGO-1表達(dá)后,干擾組神經(jīng)元突起長(zhǎng)度較對(duì)照組明顯增長(zhǎng),差異有顯著性意義(P0.05);(4)結(jié)果表明,體外培養(yǎng)的脊髓神經(jīng)干細(xì)胞具有多項(xiàng)分化潛能,LINGO-1在脊髓神經(jīng)干細(xì)胞及分化早期的神經(jīng)元、少突膠質(zhì)細(xì)胞中表達(dá),但不在星形膠質(zhì)細(xì)胞中表達(dá)。LINGO-1對(duì)分化后神經(jīng)元突起生長(zhǎng)具有負(fù)性調(diào)控作用。
[Abstract]:Background: to study the expression and effect of LINGO-1 in spinal cord neural stem cell differentiation is of great significance in regulating neural stem cell differentiation and repairing spinal cord injury. Objective: to observe the expression and biological function of LINGO-1 in the differentiation of spinal cord neural stem cells (NSCs). Methods: the neural stem cells of rat spinal cord were cultured in vitro. The dry properties of cultured cells were identified by Nestin staining and the pluripotency of differentiation was identified. LINGO-1 and Nestin (neural stem cell), 尾 -Tubulin 鈪，

本文編號(hào)：1915188