本文選題：脊髓 + 神經(jīng)干細胞　； 參考：《中國組織工程研究》2017年33期

【摘要】：背景:研究LINGO-1在脊髓神經(jīng)干細胞分化過程中的表達及效應(yīng)對調(diào)控神經(jīng)干細胞分化及修復(fù)脊髓損傷有著重要的意義。目的:觀察LINGO-1在脊髓神經(jīng)干細胞分化過程中的表達特征及生物學(xué)作用。方法:體外培養(yǎng)大鼠脊髓神經(jīng)干細胞,并對培養(yǎng)細胞進行Nestin染色干性鑒定及誘導(dǎo)分化多能性鑒定。于分化第0天及第5天進行LINGO-1與Nestin(神經(jīng)干細胞)、β-TubulinⅢ(神經(jīng)元細胞)、GFAP(星形膠質(zhì)細胞)及O4(少突膠質(zhì)細胞)免疫熒光雙染色,觀察LINGO-1的表達特征。體外培養(yǎng)的大鼠脊髓神經(jīng)干細胞分為對照組及干擾組,干擾組脊髓神經(jīng)干細胞感染LINGO-1 sh RNA慢病毒下調(diào)LINGO-1表達,對照組脊髓神經(jīng)干細胞感染Scramble-sh RNA慢病毒,于感染第5天免疫熒光染色檢測神經(jīng)元突起長度。結(jié)果與結(jié)論:(1)脊髓神經(jīng)干細胞可以分化成為神經(jīng)元、星形膠質(zhì)細胞和少突膠質(zhì)細胞;(2)LINGO-1在脊髓神經(jīng)干細胞及其分化的神經(jīng)元、少突膠質(zhì)細胞中表達,但不在星形膠質(zhì)細胞中表達;(3)下調(diào)LINGO-1表達后,干擾組神經(jīng)元突起長度較對照組明顯增長,差異有顯著性意義(P0.05);(4)結(jié)果表明,體外培養(yǎng)的脊髓神經(jīng)干細胞具有多項分化潛能,LINGO-1在脊髓神經(jīng)干細胞及分化早期的神經(jīng)元、少突膠質(zhì)細胞中表達,但不在星形膠質(zhì)細胞中表達。LINGO-1對分化后神經(jīng)元突起生長具有負性調(diào)控作用。
[Abstract]:Background: to study the expression and effect of LINGO-1 in spinal cord neural stem cell differentiation is of great significance in regulating neural stem cell differentiation and repairing spinal cord injury. Objective: to observe the expression and biological function of LINGO-1 in the differentiation of spinal cord neural stem cells (NSCs). Methods: the neural stem cells of rat spinal cord were cultured in vitro. The dry properties of cultured cells were identified by Nestin staining and the pluripotency of differentiation was identified. LINGO-1 and Nestin (neural stem cell), 尾 -Tubulin 鈪，

本文編號：1915188