本文選題：彌漫性軸索損傷 + 繼發(fā)性損傷�。� 參考：《浙江大學(xué)》2015年碩士論文

【摘要】：目的：檢測(cè)大鼠彌漫性軸索損傷后(diffuse axonal injury,DAI)髓鞘碎片在軸索損傷位點(diǎn)的堆積和清除。 方法：雄性SD大鼠25只,隨機(jī)分為實(shí)驗(yàn)組20只和對(duì)照組5只。實(shí)驗(yàn)組大鼠采用Marmarou方法制作大鼠DAI模型,分別于傷后1天、7天、14天和28天各取5只,斷頭取腦,制作冰凍切片,并采用油紅O染色檢測(cè)損傷位點(diǎn)的髓鞘碎片。對(duì)照組5只進(jìn)行假手術(shù)操作而不打擊,并采用同樣的方法處理腦組織。顯微鏡下觀察各切片,計(jì)數(shù)油紅O染色陽性顆粒的個(gè)數(shù)。 結(jié)果：對(duì)照組在皮層、海馬及腦干內(nèi)均未見明顯RO染色陽性顆粒。實(shí)驗(yàn)組中,傷后1天,以上區(qū)域腦組織內(nèi)仍未見明顯陽性顆粒。在皮層組織內(nèi),傷后7天平均每200x圖片內(nèi)陽性顆粒的數(shù)目為48.00±16.70個(gè)；到14天時(shí),增加至366.67±149.39個(gè),與7天時(shí)相比具有顯著性差異(p0.01)；至28天時(shí),陽性顆粒數(shù)目降至25.67±7.02個(gè),與7天時(shí)數(shù)目基本相當(dāng)(與28天組比,p0.01；與7天組比,p0.05)。在海馬組織內(nèi),傷后7天平均每200x圖片內(nèi)陽性顆粒的數(shù)目為11.67±3.06個(gè)；至14天時(shí)為23.33±4.16個(gè),較7天組顯著增加(p0.01)；至28天時(shí),陽性顆�；鞠�,平均數(shù)目為2.00±1.00個(gè)(與7天組及14天組相比,均為p0.01)。在腦干組織內(nèi),傷后7天平均每200x圖片內(nèi)陽性顆粒的數(shù)目為51.67±6.81個(gè)；14天時(shí)為23.67±4.93個(gè),較7天時(shí)顯著降低(p0.01),28天時(shí)平均每200x圖片內(nèi)陽性顆粒的數(shù)目與14天時(shí)基本相當(dāng),為26.69±5.69個(gè)(與7天組比p0.01,與14天組比p0.05)。 結(jié)論：DAI后在大鼠新皮層、海馬及腦干組織中存在著髓鞘碎片長(zhǎng)時(shí)間的聚積,可能通過其繼發(fā)性反應(yīng)影響DAI的預(yù)后。
[Abstract]:Aim: to investigate the accumulation and clearance of diffuse axonal injuryDAI fragments at axonal injury sites after diffuse axonal injury in rats. Methods: 25 male SD rats were randomly divided into experimental group (n = 20) and control group (n = 5). The DAI model of rats in the experimental group was made by Marmarou method. 5 rats were taken from each group on the 1st day, the 7th day, the 14th day, and the 28th day, the head was cut off, and the frozen sections were made, and the myelin sheath fragments at the injury site were detected by oil red O staining. In the control group, 5 rats underwent sham operation without attack, and the brain tissue was treated with the same method. Each section was observed under microscope and the number of oil red O positive granules was counted. Results: no RO positive granules were found in the cortex, hippocampus and brain stem in the control group. In the experimental group, 1 day after injury, no obvious positive granules were found in the brain tissue of the above areas. In cortical tissue, the average number of positive particles per 200x image was 48.00 鹵16.70 on the 7th day after injury, increased to 366.67 鹵149.39 on the 14th day, which was significantly different from that of the 7th day, and decreased to 25.67 鹵7.02 at 28 days. The number was about the same as that at 7 days (compared with 28 days group, P 0.01, and 7 days group, P 0. 05%, P 0. 05%, P 0. 05%, P 0. 05%). In hippocampal tissue, the average number of positive particles per 200x image was 11.67 鹵3.06 on the 7th day after injury, 23.33 鹵4.16 on the 14th day, which was significantly higher than that in the 7th day group, and the positive granules disappeared basically at 28 days. The average number was 2.00 鹵1.00 (compared with 7-day group and 14-day group, p0.01). In brain stem tissue, the average number of positive particles per 200x image at 7 days after injury was 51.67 鹵6.81 and 23.67 鹵4.93 on day 14, which was significantly lower than that at day 7, and the number of positive particles per 200x image on day 28 was basically the same as that at day 14. It was 26.69 鹵5.69 (p 0.01 vs 7 d and p 0.05 5) compared with 14 d group. Conclusion there is a long accumulation of myelin fragments in the neocortex, hippocampus and brain stem tissue of rats after the brain stem injury, which may influence the prognosis of DAI through its secondary reaction.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R651.15

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 孫曉川 ,唐文淵 ,鄭履平;Ultrastructural observation of effect of moderate hypothermia on axonal damage i n an animal model of diffuse axonal injury[J];Chinese Journal of Traumatology;2002年06期

2 徐如祥,易聲禹,吳聲伶,王伯云;神經(jīng)細(xì)胞Ca~(2+)通道變化及對(duì)鼠血腦屏障通透性和外傷性腦水腫的影響[J];中華神經(jīng)外科雜志;1992年01期

3 賀曉生,易聲禹,章翔,費(fèi)舟,張劍寧,梁景文,楊利孫;腦彌漫性軸索損傷軸索內(nèi)鈣超載及鈣拮抗劑的治療作用[J];中華神經(jīng)外科雜志;2000年01期

4 江基堯;;彌漫性軸突損傷的概念和病理機(jī)制[J];中華神經(jīng)外科雜志;2006年11期

，

本文編號(hào)：1892170