本文選題：半月板 + 移植��； 參考：《西北農(nóng)林科技大學(xué)》2015年碩士論文

【摘要】：目的:半月板在膝關(guān)節(jié)功能中起著關(guān)鍵作用,半月板損傷往往會(huì)導(dǎo)致骨關(guān)節(jié)炎和膝關(guān)節(jié)功能障礙。本研究通過(guò)建立犬半月板損傷模型,隨后進(jìn)行不同形式的同種異體半月板移植修復(fù),摸索有效的半月板損傷治療方法,也為人醫(yī)對(duì)半月板損傷的治療提供參考。方法:將1 6只雜種犬分為全移植組、半移植組、不移植組和正常對(duì)照組,每組4只。全移植組:對(duì)試驗(yàn)犬進(jìn)行右膝內(nèi)側(cè)半月板全切術(shù)并進(jìn)行同種異體半月板移植。半移植組:對(duì)試驗(yàn)犬進(jìn)行左膝內(nèi)側(cè)半月板前半部切除術(shù)并進(jìn)行同種異體半月板移植。不移植組:對(duì)試驗(yàn)犬進(jìn)行右膝內(nèi)側(cè)半月板全切除術(shù)和左膝內(nèi)側(cè)半月板前半部切除術(shù),切除的半月板作供體備用。正常對(duì)照組:不進(jìn)行任何操作。術(shù)后第6周對(duì)各組進(jìn)行大體觀察及膝關(guān)節(jié)功能Lysholm評(píng)分、關(guān)節(jié)液檢查、剖檢、組織學(xué)觀察、脛骨平臺(tái)軟骨Mankin’s評(píng)分。結(jié)果:術(shù)后第5周末,所有移植犬站立、行走和上臺(tái)階均趨于正常,但不移植對(duì)照犬行走時(shí)仍有輕度跛行,上臺(tái)階困難。術(shù)后第6周,膝關(guān)節(jié)功能Lysholtn評(píng)分結(jié)果顯示,全移植組和半移植組與正常對(duì)照組差異不顯著,不移植組與正常對(duì)照組差異顯著。關(guān)節(jié)液檢查無(wú)感染。移植組關(guān)節(jié)液成分和正常對(duì)照組基本一致,但不移植組炎性細(xì)胞增多。剖檢見(jiàn)供體半月板在受體內(nèi)生長(zhǎng)良好,其位置未發(fā)生變化。全移植組半月板和關(guān)節(jié)囊愈合良好,其表面有類(lèi)似滑膜的組織出現(xiàn)。半移植組供體半月板的色澤和彈性更接近正常半月板。不移植組有少量周?chē)M織長(zhǎng)入脛骨平臺(tái)邊緣,關(guān)節(jié)軟骨磨損嚴(yán)重,股骨髁表面粗糙。組織學(xué)觀察,供體半月板和受體關(guān)節(jié)囊、供體半月板和保留的受體半月板細(xì)胞融合良好,接合處組織生長(zhǎng)旺盛。脛骨平臺(tái)軟骨觀察顯示,全移植組各層分界清晰,但局部潮線彎曲;半移植組接近正常關(guān)節(jié)軟骨;不移植組軟骨表面有不規(guī)則裂隙,細(xì)胞彌散性增多,潮線紊亂。脛骨平臺(tái)軟骨Mankin’s評(píng)分顯示,全移植組和半移植組與對(duì)照組差異不顯著,不移植組與對(duì)照組差異顯著。結(jié)論:犬半月板移植手術(shù)方法是可行的。同種異體半月板移植能夠有效保護(hù)脛骨平臺(tái)軟骨、改善膝關(guān)節(jié)功能,完全可以用于治療犬半月板損傷。
[Abstract]:Objective: meniscus plays a key role in knee joint function. Meniscus injury often leads to osteoarthritis and knee joint dysfunction. In this study, a canine meniscus injury model was established, and then different forms of allograft meniscus transplantation were carried out to explore the effective meniscus injury treatment method, and to provide a reference for the treatment of meniscus injury. Methods: sixteen mongrel dogs were divided into total transplantation group, semi transplantation group, non transplantation group and normal control group, with 4 dogs in each group. Total transplantation group: the right knee medial meniscus and allogeneic meniscus transplantation were performed. Half transplantation group: left knee medial meniscus anterior resection and allograft meniscus transplantation. No transplantation group: total resection of medial meniscus of right knee and anterior resection of medial meniscus of left knee were performed. Normal control group: no operation was performed. At the 6th week after operation, gross observation and Lysholm score of knee joint function, joint fluid examination, dissection, histological observation and Mankin's score of tibial plateau cartilage were carried out in each group. Results: at the end of the 5th week after operation, all the dogs tended to stand, walk and step up to normal, but the dogs without transplantation still had mild lameness and difficulty in walking. At the 6th week after operation, the Lysholtn score of knee joint function showed that there was no significant difference between the total transplantation group and the semi-transplantation group and the normal control group, but the difference between the non-transplantation group and the normal control group was significant. No infection was detected in joint fluid. The components of joint fluid in the transplantation group were basically the same as those in the normal control group, but the inflammatory cells were increased in the non-transplantation group. The results showed that the donor meniscus grew well in the recipient body and the position of the donor meniscus did not change. The meniscus and articular capsule healed well and synovial tissue appeared on the surface of the whole transplantation group. The color and elasticity of the donor meniscus were closer to the normal meniscus in the half transplantation group. In the non-transplantation group, a small amount of surrounding tissue grew into the edge of the tibial plateau, the articular cartilage was badly worn and the surface of the femoral condyle was rough. Histologically, the donor meniscus and the recipient joint capsule, the donor meniscus and the reserved recipient meniscus cells fused well, and the tissue at the junction grew well. The observation of tibial plateau cartilage showed that the boundary of each layer was clear, but the local tide line was bent; the half transplantation group was close to normal articular cartilage; the cartilage surface of non-transplantation group had irregular fissure, cell dispersion increased and tidal line disorder. The Mankin's score of tibial plateau cartilage showed that there was no significant difference between the total transplantation group and the half transplantation group and the control group, but the difference between the non-transplantation group and the control group was significant. Conclusion: the method of meniscus transplantation is feasible. The allograft meniscus transplantation can effectively protect the tibial plateau cartilage and improve the knee joint function. It can be used in the treatment of canine meniscus injury.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R687.4

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 張浩;蔡振杰;俞世強(qiáng);趙璧君;張近寶;李利;段云燕;;自體心耳肌細(xì)胞移植對(duì)心功能的影響[J];心臟雜志;2007年02期

2 ;[J];;年期

相關(guān)會(huì)議論文 前1條

1 王新平;谷芙蓉;曾憲珠;李晨華;王世民;張文治;秦進(jìn)喜;;實(shí)驗(yàn)性腦梗死后不同時(shí)間移植神經(jīng)干細(xì)胞效果研究[A];第九次全國(guó)神經(jīng)病學(xué)學(xué)術(shù)大會(huì)論文匯編[C];2006年

相關(guān)博士學(xué)位論文 前1條

1 文川;胎鼠及新生鼠外周血（FNPB）輸注對(duì)MRL/lpr小鼠作用的實(shí)驗(yàn)研究[D];中南大學(xué);2013年

相關(guān)碩士學(xué)位論文 前4條

1 余關(guān)林;犬同種異體半月板移植研究[D];西北農(nóng)林科技大學(xué);2015年

2 郭世強(qiáng);自體BM-MSCs移植治療缺血性心臟病時(shí)間窗對(duì)心功能影響的實(shí)驗(yàn)研究[D];蘇州大學(xué);2007年

3 曾憲珠;腦內(nèi)不同部位神經(jīng)干細(xì)胞移植治療腦梗塞的實(shí)驗(yàn)研究[D];天津醫(yī)科大學(xué);2005年

4 唐一鋒;HMGB1聯(lián)合MSCs移植對(duì)急性心肌梗死大鼠心臟功能影響的實(shí)驗(yàn)研究[D];遵義醫(yī)學(xué)院;2014年

，

本文編號(hào)：1883584