本文選題：焦脫鎂葉綠酸-a甲酯 + 光動力療法��； 參考：《重慶醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:本研究以體外培養(yǎng)的骨肉瘤MG63細(xì)胞為研究對象,擬用MPPa介導(dǎo)的光動力療法處理骨肉瘤MG63細(xì)胞,探討焦脫鎂葉綠酸-a甲酯介導(dǎo)的光動力療法對人骨肉瘤MG-63細(xì)胞遷移及侵襲的影響及其可能機(jī)制。為MPPa-PDT用于骨肉瘤遠(yuǎn)處轉(zhuǎn)移抑制及臨床治療提供實(shí)驗(yàn)理論基礎(chǔ)。方法:將對數(shù)生長期骨肉瘤MG-63細(xì)胞分為空白對照組(Control組)、單純MPPa藥物組(MPPa組)、單純光照組(LED組)以及MPPa-PDT實(shí)驗(yàn)組(MPPa-PDT組)。人骨肉瘤MG-63細(xì)胞經(jīng)MPPa-PDT處理后采用CCK8檢測不同時(shí)間點(diǎn)的細(xì)胞活性,明確MPPa-PDT對骨肉瘤MG63細(xì)胞的藥物毒性效應(yīng);劃痕實(shí)驗(yàn)及Transwell小室模型實(shí)驗(yàn)檢測細(xì)胞遷移、侵襲能力,探明MPPa-PDT能否抑制骨肉瘤MG63細(xì)胞的遷移及侵襲能力;通過Western blot檢測遷移侵襲標(biāo)志性蛋白E-鈣粘蛋白(E-cad)和基質(zhì)金屬蛋白酶(MMP)-2、-9蛋白的表達(dá)水平,以明確MPPa-PDT是否抑制了E-鈣粘蛋白(E-cad)和基質(zhì)金屬蛋白酶(MMP)-2、-9蛋白的表達(dá)。結(jié)果:MPPa-PDT處理MG-63細(xì)胞以后,細(xì)胞活性明顯降低,在12、24及48小時(shí)MPPa-PDT組細(xì)胞增殖能力較Control組、MPPa組、LED組存在明顯抑制作用。通過MPPa-PDT處理后,我們可以觀察到MPPa-PDT組細(xì)胞的劃痕愈合能力、遷移活力及侵襲能力較Control組、MPPa組、LED組明顯下降,MPPa-PDT對骨肉瘤MG-63細(xì)胞的遷移侵襲有抑制作用。Western blot檢測結(jié)果則顯示MPPa-PDT組的E-cad明顯高于Control組、MPPa組及LED組,而MMP-2、MMP-9的表達(dá)則明顯低于其他三組(P0.05),在蛋白水平,MPPa-PDT可對遷移侵襲關(guān)鍵蛋白起抑制作用。結(jié)論:MPPa-PDT抑制人骨肉瘤MG-63細(xì)胞的侵襲和遷移能力;上調(diào)E-cad的表達(dá)、下調(diào)MMP-2,-9的表達(dá)可能是其作用機(jī)制之一。
[Abstract]:Objective: in this study, osteosarcoma MG63 cells cultured in vitro were used to treat MG63 cells with MPPa mediated photodynamic therapy. To investigate the effect of methyl pyromagnesia-a photodynamic therapy on the migration and invasion of human osteosarcoma MG-63 cells and its possible mechanism. To provide experimental theoretical basis for the suppression of distant metastasis and clinical treatment of osteosarcoma by MPPa-PDT. Methods: MG-63 cells in logarithmic phase of osteosarcoma were divided into control group (control group), MPPa drug group (MPPA group), light group (LED group) and MPPa-PDT experimental group (MPPa-PDT group). Human osteosarcoma MG-63 cells treated with MPPa-PDT were treated with CCK8 to determine the cytotoxicity of MPPa-PDT to MG63 cells at different time points, scratch test and Transwell chamber model test were used to detect cell migration and invasion ability. To determine whether MPPa-PDT can inhibit the migration and invasion of osteosarcoma MG63 cells, and to detect the expression levels of E-cad- and MMP-2P protein by Western blot. In order to determine whether MPPa-PDT inhibits the expression of E-cadand and MMP-2P-9 protein of matrix metalloproteinases (MMP) and matrix metalloproteinase (MMP). Results the cell activity of MG-63 cells was significantly decreased after treated with 1: MPPa-PDT, and the proliferation ability of MPPa-PDT group was significantly inhibited than that of Control group at 24 and 48 hours. After treatment with MPPa-PDT, we can observe the ability of scratch healing of MPPa-PDT cells. Migration activity and invasion ability were significantly lower in Control group than those in Control group. Western blot analysis showed that E-cad in MPPa-PDT group was significantly higher than that in Control group and LED group. The expression of MMP-2 and MMP-9 was significantly lower than that of the other three groups, and MPPa-PDT could inhibit the key proteins of migration and invasion at the protein level. Conclusion the invasion and migration ability of human osteosarcoma MG-63 cells was inhibited by WMPPa-PDT, and the up-regulation of E-cad and the down-regulation of MMP-2 ~ (-9) expression may be one of the mechanisms of the inhibition of invasion and migration of human osteosarcoma MG-63 cells.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R738

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 黃秋;涂平華;歐云生;劉渤;陶勇;尹航;;MPPa-PDT誘導(dǎo)人骨巨細(xì)胞瘤細(xì)胞自噬[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2015年17期

2 李黎波;李文敏;項(xiàng)蕾紅;秦中平;王洛偉;林盡染;馬英;羅榮城;黃正;;光動力療法在中國的應(yīng)用與臨床研究[J];中國激光醫(yī)學(xué)雜志;2012年05期

3 熊亞林;林群賢;王毅雋;;中老年肱骨近端骨折不同治療方法療效分析[J];中國骨與關(guān)節(jié)損傷雜志;2011年01期

4 許川山;Albert Wing Nang Leung;楊青;;MPPa光動力作用對鼻咽癌細(xì)胞生長抑制的初步研究[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2006年14期

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