本文選題：辛伐他汀 + 骨密度　； 參考：《醫(yī)學(xué)研究生學(xué)報(bào)》2017年02期

【摘要】：目的辛伐他汀作為降脂類藥物表現(xiàn)出一定的促進(jìn)骨形成作用。文中旨在探討辛伐他汀對卵巢切除大鼠椎體骨的骨量、微觀結(jié)構(gòu)以及椎間盤退變的作用。方法所有大鼠采用隨機(jī)數(shù)字表法分為3組:假手術(shù)組,模型組、辛伐他汀組,每組10只。假手術(shù)組切開暴露卵巢,但不予切除;其余2組大鼠均行雙側(cè)卵巢切除術(shù),并分別灌胃給予等滲鹽水和辛伐他汀干預(yù)(10 mg/m L)溶于雙蒸水制成混懸液,給藥劑量5 mg/(kg·d)。取大鼠L3椎體行micro-CT分析其微觀結(jié)構(gòu),取L5-6椎體,雙能X線檢測其骨密度后常規(guī)脫鈣包埋,制備切片并行VG染色,經(jīng)組織學(xué)觀察和半定量評分評估L5-6椎間盤退變情況。結(jié)果與假手術(shù)組L5、L6、L5-6骨密度[(0.2933±0.011 0)、(0.290 7±0.015 0)、(0.286 0±0.011 5)g/cm2]比較,模型組[(0.242 3±0.008 1)、(0.239 5±0.006 1)、(0.238 0±0.005 9)g/cm2]降低,辛伐他汀組[(0.2598±0.0249)、(0.2572±0.0121)、(0.2528±0.0126)g/cm2]降低(P0.05);與模型組比較,辛伐他汀組L5、L6、L5-6骨密度升高(P0.05)。三維重建圖顯示,與假手術(shù)組比較,模型組和辛伐他汀組骨小梁稀疏,連結(jié)性較差。BV/TV、Tb.N降低,Tb.Sp升高(P0.05);辛伐他汀組Tb.Sp升高(P0.05)。椎間盤組織學(xué)觀察及評分:假手術(shù)組脊索細(xì)胞較多、細(xì)胞外基質(zhì)較豐富、纖維環(huán)排列整齊。模型組、辛伐他汀組相比于假手術(shù)組,脊索細(xì)胞數(shù)量減少并被軟骨樣細(xì)胞取代,有黏液樣改變,纖維環(huán)與髓核邊界模糊、排列紊亂。模型組、辛伐他汀組半定量評分[(4.35±0.90)分、(3.53±0.42)分]顯著高于假手術(shù)組[(2.48±0.92)分],差異有統(tǒng)計(jì)學(xué)意義(P0.05),辛伐他汀與模型組比較差異無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論卵巢切除大鼠不僅椎體骨量丟失,椎間盤也有退變表現(xiàn);辛伐他汀可以部分抑制卵巢切除大鼠腰椎骨量丟失,但對該模型椎間盤退變無顯著影響。
[Abstract]:Objective simvastatin, as a lipid lowering drug, showed a certain effect on bone formation. The purpose of this study was to explore the effect of simvastatin on bone mass, microstructure and disc degeneration in ovariectomized rats. Methods all rats were divided into 3 groups by random digital table method: artificial hand group, model group, simvastatin group, 10 rats in each group. In the operation group, the ovaries were cut and exposed, but the other 2 groups of rats were treated with bilateral oophorectomy, and they were treated with isotonic saline and simvastatin (10 mg/m L) to dissolve in double water to make a suspension, 5 mg/ (kg. D). The microstructures of the L3 vertebral body of rats were analyzed by micro-CT, and L5-6 vertebral body was taken and the double energy X ray was used to detect the bone density. After the routine decalcification, the L5-6 disc degeneration was evaluated by histological observation and semi quantitative score. The results were compared with the L5, L6, L5-6 bone density of the sham operation group [(0.2933 + 0.0110), (0.2907 + 0.0150), (0.2860 + 0.0115) g/cm2], and the model group [0.2423 + 0.0081), (0.2395 + 0.0061), (0.2380 + 0.0059) g/cm 2] decreased, simvastatin Group [(0.2598 + 0.0249), (0.2572 + 0.0121), (0.2528 + 0.0126) g/cm2]) decreased (P0.05). Compared with the model group, L5, L6, L5-6 bone density increased (P0.05) in the simvastatin group. The three-dimensional reconstruction map showed that the bone trabecula in the model group and simvastatin group was sparse, the connective poor.BV/TV, Tb.N decreased, Tb.Sp increased (P0.05). The Tb.Sp of the simvastatin group increased (P0.05). The histological observation and score of the intervertebral disc: the sham operation group had more chord cells, more abundant extracellular matrix and neatly arrayed fibrous ring. The semi quantitative score of the model group and simvastatin Group [(4.35 + 0.90), (3.53 + 0.42)] was significantly higher than that of the sham operation group ((2.48 + 0.92)), the difference was statistically significant (P0.05). There was no significant difference between the simvastatin and the model group (P0.05). Simvastatin can partially inhibit lumbar bone loss in ovariectomized rats, but has no significant effect on intervertebral disc degeneration in this model.

【作者單位】： 華北理工大學(xué)醫(yī)學(xué)實(shí)驗(yàn)研究中心;華北理工大學(xué)附屬醫(yī)院整形外科;華北理工大學(xué)基礎(chǔ)醫(yī)學(xué)院;
【基金】：河北省自然科學(xué)基金(H2016209176,H2013209255,H2013209257) 河北省高等學(xué)�？茖W(xué)技術(shù)研究青年基金(QN20131007) 唐山市科學(xué)技術(shù)研究與發(fā)展計(jì)劃(13130281z)
【分類號】：R681.5

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