本文選題：IL-1α + BMP-7��； 參考：《川北醫(yī)學(xué)院》2015年碩士論文

【摘要】：目的:通過(guò)檢測(cè)IL-1α及BMP-7在人類腰椎退變性黃韌帶肥厚中的表達(dá)情況,探索其在黃韌帶肥厚中的作用機(jī)制,為黃韌帶肥厚引起的腰椎狹窄癥及黃韌帶骨化的干預(yù)提供新的思路。方法:1.收集2013年1月—2014年10月在川北醫(yī)學(xué)院附屬醫(yī)院骨科住院治療的腰椎骨折和腰椎管狹窄患者黃韌帶標(biāo)本共20例。其中腰椎骨折患者10例為對(duì)照組,年齡18-30歲,男8例,女2例,既往健康,排除其他慢性疾病,經(jīng)影像學(xué)證實(shí)無(wú)腰椎黃韌帶肥厚;腰椎管狹窄癥患者10例為實(shí)驗(yàn)組,年齡55-65歲,男4例,女6例,既往健康,排除其他慢性疾病,經(jīng)影像學(xué)證實(shí)腰椎黃韌帶0.5cm。2.術(shù)中取椎板間整塊黃韌帶,測(cè)量標(biāo)本厚度,常規(guī)石蠟切片,HE染色觀察成纖維細(xì)胞并計(jì)數(shù),免疫組織化學(xué)法、RT-PCR及Western blotting檢測(cè)實(shí)驗(yàn)組和對(duì)照組中黃韌帶IL-1α及BMP-7,各組測(cè)得的數(shù)據(jù)均用均數(shù)±標(biāo)準(zhǔn)差(sx±)表示,用SPSS19.0軟件分析包進(jìn)行統(tǒng)計(jì)學(xué)分析,計(jì)數(shù)資料卡方檢驗(yàn),計(jì)量資料t檢驗(yàn)P0.05認(rèn)為差異具有統(tǒng)計(jì)學(xué)意義。結(jié)果:1.大體標(biāo)本形態(tài)學(xué)觀察:對(duì)照組標(biāo)本表面光滑、透亮、柔軟,和周邊組織邊界清楚,易于分離,切下后回縮快,呈卷曲狀,彈性良好,均數(shù)標(biāo)準(zhǔn)差0.215±0.37(cm)。實(shí)驗(yàn)組標(biāo)本表面欠光滑、周圍粘連明顯,質(zhì)地致密、堅(jiān)韌、不易分離,未發(fā)現(xiàn)鈣化和骨化,均數(shù)標(biāo)準(zhǔn)差0.678±0.064(cm)。兩組間比較差異有統(tǒng)計(jì)學(xué)意義P0.05。2.HE染色:對(duì)照組黃韌帶纖維大小相近,長(zhǎng)軸排列,表面光滑,纖維較細(xì),呈波浪狀,其主要成分為彈力纖維,纖維方向與黃韌帶的長(zhǎng)軸平行,并有分支發(fā)出,相互連接,形成網(wǎng)狀結(jié)構(gòu);成纖維細(xì)胞計(jì)數(shù)27.00±5.18/HP。實(shí)驗(yàn)組黃韌帶纖維粗細(xì)不均,排列紊亂,纖維斷裂,彈性纖維減少,結(jié)構(gòu)變形,功能活躍的成纖維細(xì)胞增多,未發(fā)現(xiàn)鈣化及骨化;成纖維細(xì)胞計(jì)數(shù)74.40±7.26/HP。兩組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。3.免疫組織化學(xué)法檢測(cè):IL-1α在對(duì)照組和實(shí)驗(yàn)組陽(yáng)性細(xì)胞百分比分別為40%和90%,BMP-7在對(duì)照組和實(shí)驗(yàn)組陽(yáng)性細(xì)胞百分比分別為10%和90%,兩組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。4.實(shí)時(shí)熒光定量PCR法檢測(cè):各黃韌帶標(biāo)本中基因擴(kuò)增曲線、融解曲線良好。黃韌帶組織學(xué)研究發(fā)現(xiàn),IL-1α和BMP-7m RNA在實(shí)驗(yàn)組中的表達(dá)均明顯高于對(duì)照組(P0.05)。5.Western blot檢測(cè):實(shí)驗(yàn)組IL-1α和BMP-7的表達(dá)水平均較對(duì)照組顯著增強(qiáng),差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:1.IL-1α表達(dá)增加可能是導(dǎo)致黃韌帶退變的重要機(jī)制之一2.BMP-7表達(dá)增加可能是導(dǎo)致黃韌帶退變的重要機(jī)制之一。
[Abstract]:Objective: to investigate the expression of IL-1 偽 and BMP-7 in the hypertrophy of degenerative ligamentum flavum in human lumbar vertebrae, and to explore the mechanism of its role in the hypertrophy of ligamentum flavum, so as to provide a new idea for the intervention of lumbar stenosis and ossification of ligamentum flavum caused by hypertrophy of ligamentum flavum. Method 1: 1. From January 2013 to October 2014, 20 specimens of ligamentum flavum were collected from lumbar fractures and lumbar spinal stenosis treated in orthopaedic department of affiliated Hospital of North Sichuan Medical College. There were 10 cases of lumbar vertebrae fracture as control group, aged 18-30 years, male 8 cases, female 2 cases, past health, excluding other chronic diseases, no lumbar ligamentum flavum hypertrophy was confirmed by imaging, and 10 cases of lumbar spinal stenosis were treated as experimental group, aged 55-65 years. Male 4 cases, female 6 cases, past health, excluding other chronic diseases, lumbar ligamentum flavum 0.5 cm. 2. The interlaminar ligamentum flavum was taken during the operation and the thickness of the specimen was measured. The fibroblasts were observed and counted by HE staining in routine paraffin sections. Immunohistochemical method was used to detect IL-1 偽 and BMP-7 in experimental and control groups by RT-PCR and Western blotting. The data of each group were expressed as mean 鹵standard deviation (sx 鹵). Statistical analysis was carried out with SPSS19.0 software analysis kit, and counting data was chi-square test. Measurement data t test P0.05 that the difference was statistically significant. The result is 1: 1. Morphological observation of gross specimens: the surface of the control group was smooth, transparent and soft, and the border with the surrounding tissue was clear and easy to be separated. After cutting, the specimen was curled, with good elasticity, and the mean standard deviation was 0.215 鹵0.37 cm ~ (-1) 路cm ~ (-1). In the experimental group, the surface of the specimen was not smooth, the adhesion around it was obvious, the texture was dense, tough and difficult to separate, no calcification and ossification were found, and the mean standard deviation was 0.678 鹵0.064 cm ~ (-1). There were significant differences in P0.05.2.HE staining between the two groups: in the control group, the fibers of the ligamentum flavum were similar in size, arranged in long axis, smooth in surface, fine in fiber and wavy in shape. The main component of the fibers was elastic fibers, and the direction of the fibers was parallel to the long axis of the ligamentum flavum. The number of fibroblasts was 27.00 鹵5.18% HP. In the experimental group, the fibers of ligamentum flavum were uneven in thickness, disordered in arrangement, broken in fiber, decreased in elastic fiber, deformed in structure, increased in number of active fibroblasts, no calcification and ossification were found, and the number of fibroblasts was 74.40 鹵7.26% HP. The difference between the two groups was statistically significant (P 0.05). The percentage of positive cells in control group and experimental group were 40% and 90% respectively by immunohistochemical method. The percentage of positive cells of BMP-7 in control group and experimental group were 10% and 90%, respectively. The difference between the two groups was statistically significant. Real-time fluorescence quantitative PCR assay showed that the curve of gene amplification in ligamentum flavum was good. Histological study of ligamentum flavum showed that the expressions of IL-1 偽 and BMP-7m RNA in the experimental group were significantly higher than those in the control group (P 0.05A). 5. Western blot: the expression levels of IL-1 偽 and BMP-7 in the experimental group were significantly higher than those in the control group (P 0.05). Conclusion 1. The increased expression of IL-1 偽 may be one of the important mechanisms leading to the degeneration of ligamentum flavum. The increase of 2.BMP-7 expression may be one of the important mechanisms leading to degeneration of ligamentum flavum.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R681.5

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