天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

當(dāng)前位置:主頁(yè) > 醫(yī)學(xué)論文 > 外科論文 >

人臍帶Wharton’s Jelly間充質(zhì)干細(xì)胞治療老年肌肉衰減綜合征的實(shí)驗(yàn)研究

發(fā)布時(shí)間:2018-04-10 12:17

  本文選題:人臍帶Wharton’s + Jelly間充質(zhì)干細(xì)胞。 參考:《青島大學(xué)》2017年碩士論文


【摘要】:目的:利用人臍帶Wharton’s Jelly間充質(zhì)干細(xì)胞(h UCMSCs)移植治療老年肌肉衰減綜合征模型小鼠,探討干細(xì)胞移植治療的方法及可行性。方法:利用后肢懸提技術(shù)建立老年肌肉衰減綜合征模型。取24月齡C57BL/6雄性小鼠24只,隨機(jī)分為靜脈注射干細(xì)胞組、肌肉注射干細(xì)胞組、靜脈注射漢克氏液組、肌肉注射漢克氏液組,每組6只。用胰酶消化法與差速貼壁法培養(yǎng)h UCMSCs,傳代3次后分別利用腓腸肌局部和尾靜脈注射的方法進(jìn)行h UCMSCs移植治療。通過(guò)比較最大等距跖屈肌力、腓腸肌的肌肉濕重、肌纖維的橫截面積、I型肌肉纖維的占比等指標(biāo)對(duì)h UCMSCs移植的療效進(jìn)行評(píng)價(jià)。結(jié)果:1.胰酶消化與差速貼壁法培養(yǎng)h UCMSCs,可獲得穩(wěn)定傳代及足量的細(xì)胞,能夠保證實(shí)驗(yàn)的需求。2.后肢懸提2周導(dǎo)致老年小鼠的體重下降,小鼠重新行走并接受h UCMSCs移植8周后,體重仍繼續(xù)下降。3.后肢懸提可以顯著降低老年小鼠最大等距跖屈肌力,h UCMSCs移植可以顯著改善老年小鼠的最大等距跖屈肌力,局部肌肉內(nèi)h UCMSCs移植效果要好于尾靜脈注射移植。4.h UCMSCs移植顯著增加腓腸肌的濕重、肌纖維的橫截面積及腓腸肌肌肉組織中I型肌肉纖維的占比,局部肌肉注射移植同樣優(yōu)于尾靜脈注射移植。結(jié)論:1.hUCMSCs易于體外培養(yǎng)擴(kuò)增,可以通過(guò)體外注射進(jìn)行細(xì)胞移植2.hUCMSCs移植可顯著增加腓腸肌的濕重,并顯著改善老年小鼠的下肢肌力,提示h UCMSCs移植在老年肌肉衰減綜合征模型動(dòng)物體內(nèi)能夠起到一定的治療作用。
[Abstract]:Objective: to study the feasibility of transplantation of Wharton's Jelly mesenchymal stem cells from human umbilical cord to treat senile muscle attenuation syndrome model mice.Methods: the model of senile muscle attenuation syndrome was established by hind limb suspension technique.Twenty-four C57BL/6 male mice aged 24 months were randomly divided into three groups: intravenous stem cell group, intramuscular stem cell injection group, Hank's fluid group and Hank's fluid group.HUCMSCs were cultured with trypsin digestion and differential adherent method. After three passages, the local and caudal vein injection of gastrocnemius was used for the transplantation of h UCMSCs respectively.The effect of h UCMSCs transplantation was evaluated by comparing the maximum isometric flexor muscle strength, muscle wet weight of gastrocnemius muscle, and the ratio of muscle fiber cross-sectional area to type I muscle fiber.The result is 1: 1.By trypsin digestion and differential adherence method, stable passage and sufficient number of cells could be obtained.The hind limb suspension for 2 weeks resulted in the weight loss of the aged mice, and the weight loss continued to decrease 8 weeks after the mice walked again and received h UCMSCs transplantation for 8 weeks.The hind limb suspension could significantly reduce the maximal isometric flexor muscle strength of the aged mice and the maximum isometric flexor muscle strength of the aged mice could be significantly improved by the transplantation of h UCMSCs.The effect of local intramuscular h UCMSCs transplantation was better than that of tail vein injection. 4. H UCMSCs transplantation significantly increased the wet weight of gastrocnemius muscle, the cross sectional area of muscle fiber and the proportion of type I muscle fiber in gastrocnemius muscle tissue.Local intramuscular injection was also superior to caudal vein transplantation.Conclusion\\\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\%\% UCMSCs can be easily cultured and amplified in vitro. 2.hUCMSCs transplantation can significantlyThese results suggest that h UCMSCs transplantation can play a therapeutic role in senile muscle attenuation syndrome model animals.
【學(xué)位授予單位】:青島大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2017
【分類號(hào)】:R685

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 余志斌;;去負(fù)荷骨骼肌恢復(fù)期肌節(jié)損傷的分子機(jī)制[J];空軍醫(yī)學(xué)雜志;2013年02期

2 劉小璇;樊東升;張俊;鄭菊陽(yáng);馬鐵民;;比較不同方式移植的人間充質(zhì)干細(xì)胞在大鼠失神經(jīng)支配的骨骼肌的分布及對(duì)坐骨神經(jīng)損傷的影響[J];北京大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2008年02期

3 高放,余志斌,馮漢忠,程九華;懸吊大鼠后肢骨骼肌等長(zhǎng)收縮功能變化的動(dòng)態(tài)特征[J];中華航空航天醫(yī)學(xué)雜志;2004年03期

相關(guān)博士學(xué)位論文 前1條

1 朱新煒;成骨誘導(dǎo)和移植途徑對(duì)人臍帶間充質(zhì)干細(xì)胞治療大鼠骨損傷的影響[D];山東大學(xué);2014年



本文編號(hào):1731163

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/yixuelunwen/waikelunwen/1731163.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶da083***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com
日本二区三区在线播放| 五月天婷亚洲天婷综合网| 欧美午夜一区二区福利视频| 中文字日产幕码三区国产| 欧美又大又黄刺激视频| 日本视频在线观看不卡| 亚洲国产黄色精品在线观看| 国产高清视频一区不卡| 在线日韩欧美国产自拍| 亚洲最新av在线观看| 麻豆看片麻豆免费视频| 少妇一区二区三区精品| 黄色av尤物白丝在线播放网址| 国产又粗又猛又长又黄视频| 中文久久乱码一区二区| 九九热视频免费在线视频| 99国产高清不卡视频| 美女激情免费在线观看| 在线观看免费视频你懂的| 成人精品亚洲欧美日韩| 精品欧美日韩一二三区| 日韩精品中文在线观看| 欧美一区二区三区五月婷婷| 人妻中文一区二区三区| 欧美精品一区二区水蜜桃| 欧美成人黄色一区二区三区| 国产欧美精品对白性色| 国产又色又粗又黄又爽| 亚洲最新中文字幕一区| 五月婷日韩中文字幕四虎| 高潮少妇高潮久久精品99| 日韩性生活视频免费在线观看 | 日韩国产精品激情一区| 中文字幕亚洲精品在线播放| 五月婷婷六月丁香亚洲| 91午夜少妇极品福利| av在线免费观看在线免费观看| 国内女人精品一区二区三区| 日本中文在线不卡视频| 国产在线小视频你懂的| 在线精品首页中文字幕亚洲|