本文選題：球海綿體肌反射　切入點：骶髓缺血損傷　出處：《中國神經(jīng)精神疾病雜志》2017年07期

【摘要】：目的建立公兔不同水平骶髓缺血損傷動物模型并應(yīng)用球海綿體肌反射(bulbocavernosus sphincter reflex,BCR)對模型進行評估。方法 36只新西蘭大白兔公兔隨機區(qū)組法分為實驗組(n=30)和對照組(n=6),實驗組按照尾頭方向結(jié)扎左腎動脈和動脈分叉間腰動脈的水平分為1根、2根、3根、4根和5根組共5亞組(n=6),分別結(jié)扎1根、2根、3根、4根和5根腰動脈,記錄血管結(jié)扎后2 h及2 d的BCR波,分別在麻醉清醒和血管結(jié)扎后2 d進行運動功能評分,2 d后取骶髓HE切片觀察。對照組(n=6)用于排除麻醉、手術(shù)對BCR的影響。結(jié)果對照組麻醉和手術(shù)后不同時間的波幅應(yīng)用單組重復測量數(shù)據(jù)的方差分析,差異均無統(tǒng)計學意義(F=1.234和F=1.492,均P0.05);對照組麻醉和手術(shù)后不同時間的潛伏期應(yīng)用單組重復測量數(shù)據(jù)的方差分析,差異均無統(tǒng)計學意義(F=0.911和F=3.80,均P0.05)。實驗組術(shù)前、結(jié)扎后2 h和結(jié)扎后2 d三個時間點BCR波幅與基線波幅的百分比值,經(jīng)單因素重復測量數(shù)據(jù)的方差分析差異有統(tǒng)計學意義(F=1029.943,P0.05);實驗組術(shù)前、結(jié)扎后2h和結(jié)扎后2 d三個時間點BCR潛伏期,經(jīng)單因素重復測量數(shù)據(jù)的方差分析,結(jié)果差異有統(tǒng)計學意義(F=22.711,P0.05)。1根組、2根組手術(shù)前后和3根組術(shù)后2 d運動功能評分無變化;3根組麻醉清醒后和4根、5根組手術(shù)后不同時間運動功能評分與對照組和手術(shù)前經(jīng)Wilcoxon秩和檢驗均P0.05,差異均有統(tǒng)計學意義。結(jié)論通過尾頭方向分別結(jié)扎左腎動脈和動脈分叉間不同水平的腰動脈可以建立不同程度的骶髓缺血損傷動物模型。
[Abstract]:Objective to establish an animal model of sacral spinal cord ischemia injury in male rabbits at different levels and evaluate the model by bulbocavernosus sphincter reflexus.Methods Thirty-six New Zealand white rabbits were randomly divided into two groups: the experimental group (30) and the control group (6 cases). The experimental group was divided into two groups according to the level of ligating left renal artery and the artery bifurcation lumbar artery in the direction of tail head: 1, 2, 3, 3, 4 and 5, respectively.A total of 5 subgroups were ligated, including 1, 2, 3, 4 and 5 lumbar arteries, respectively.The BCR waves were recorded at 2 hours and 2 days after ligation. The motor function scores were scored 2 days after anesthesia and 2 days after vascular ligation. The HE sections of the sacral spinal cord were taken for observation.Control group (n = 6) was used to exclude the effect of anesthesia and operation on BCR.Results in the control group, the amplitudes at different times after anesthesia and operation were analyzed by ANOVA.There was no significant difference (P < 0.05) between the two groups (P < 0.01), but there was no significant difference between the two groups (P < 0.05). The latency of anesthesia and operation in the control group was analyzed by ANOVA of repeated measurements at different time after anaesthesia and operation. There was no significant difference between the two groups (P < 0.01), but there was no significant difference between the two groups (P < 0.05).The percentage of BCR amplitude and baseline amplitude at three time points before ligation, 2 hours after ligation and 2 days after ligation were significantly different from baseline amplitude by ANOVA of single factor repeated measurement data.The latency of BCR at 2 hours after ligation and 2 days after ligation was analyzed by ANOVA.Results there was significant difference in motor function score between two groups and three groups before and after operation and 2 days after operation. Motor function scores of 3 groups after anesthesia and 4 groups with 5 roots after operation were similar to those of control group and hand group at different time after operation.The Wilcoxon rank sum test showed significant difference before operation (P 0.05).Conclusion the animal model of sacral spinal cord ischemia injury can be established by ligating the left renal artery and the lumbar artery at different levels in the direction of the tail head.
【作者單位】： 青島大學附屬醫(yī)院病理科;山東省臨沂市人民醫(yī)院神經(jīng)外科;
【基金】：山東醫(yī)藥衛(wèi)生科技發(fā)展計劃面上項目(編號:2015WS0375)
【分類號】：R-332;R651.2
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本文編號：1690652