本文選題：骨移植　切入點(diǎn)：干細(xì)胞　出處：《中國(guó)組織工程研究》2017年14期

【摘要】：背景:音猬因子作為一種Hedgehog信號(hào)蛋白,具有促使骨組織發(fā)育、生長(zhǎng)及重構(gòu)等生理功能。目的:觀察音猬因子修飾的納米晶膠原基骨復(fù)合骨髓間質(zhì)干細(xì)胞修復(fù)大鼠股骨缺損的效果。方法:將48只SD大鼠隨機(jī)分為4組,均建立股骨缺損模型,造模成功后14 d,實(shí)驗(yàn)組于骨缺損處植入音猬因子修飾的納米晶膠原基骨-骨髓間充質(zhì)干細(xì)胞復(fù)合物,支架組于骨缺損處植入納米晶膠原基骨,細(xì)胞支架組植入納米晶膠原基骨-骨髓間充質(zhì)干細(xì)胞復(fù)合物,空白對(duì)照組不植入任何材料。修復(fù)后3,6,9,12周,進(jìn)行骨缺損區(qū)X射線檢查、骨密度及骨組織病理切片觀察。結(jié)果與結(jié)論:(1)X射線檢查:實(shí)驗(yàn)組修復(fù)后不同時(shí)間點(diǎn)的Lane-Sandhu X射線評(píng)分、骨密度值均高于其余3組(P0.05);(2)蘇木精-伊紅染色:修復(fù)后12周,支架組有少量骨組織形成,無骨髓形成;細(xì)胞支架組有少量骨組織形成,無骨髓形成,可見殘留支架;實(shí)驗(yàn)組植入物完全吸收,成熟骨組織和髓腔形成,腔內(nèi)有骨髓形成;(3)掃描電鏡觀察:修復(fù)后12周,支架組有不規(guī)則排列的骨纖維和大量骨陷下窩;細(xì)胞支架組可見大量成骨細(xì)胞,但骨纖維仍然不規(guī)則;實(shí)驗(yàn)組有大量排列規(guī)則的骨小梁,可見哈佛系統(tǒng);(4)結(jié)果表明:音猬因子修飾的納米晶膠原基骨復(fù)合骨髓間質(zhì)干細(xì)胞可促進(jìn)骨缺損的修復(fù)。
[Abstract]:Background: as a Hedgehog signal protein, Hedgehog plays an important role in bone tissue development. Objective: to observe the effect of nanocrystalline collagen modified bone marrow mesenchymal stem cells (BMSCs) modified by hedgehog factor on the repair of femur defect in rats. Methods: 48 SD rats were randomly divided into 4 groups to establish femoral defect models. On the 14th day after successful modeling, the experimental group was implanted with nanocrystalline collagen-bone mesenchymal stem cell complex modified by hedgehog factor in the bone defect, and the scaffold group was implanted with nanocrystalline collagen-derived bone in the bone defect. The scaffold group was implanted with nanocrystalline collagen-bone mesenchymal stem cell complex, while the blank control group was not implanted with any material. Results and conclusion the Lane-Sandhu X-ray score at different time points after repair in the experimental group was higher than that in the other three groups (P0.05 / 2) hematoxylin-eosin staining: 12 weeks after repair, the bone mineral density of the experimental group was higher than that of the other three groups. The scaffold group had a small amount of bone tissue formation, no bone marrow formation, no bone marrow formation, there was no bone marrow formation, and the experimental group had completely absorbed the implants, and the mature bone tissue and medullary cavity were formed. Scanning electron microscope observation: at 12 weeks after repair, there were irregular arrangement of bone fibers and a large number of bone lacunae in the scaffold group, a large number of osteoblasts were observed in the cell scaffold group, but the bone fibers were still irregular in the scaffold group. There were a large number of regular trabeculae in the experimental group. The results showed that the Hedgehog factor modified nanocrystalline collagen-based bone and bone marrow mesenchymal stem cells could promote the repair of bone defect.
【作者單位】： 中山大學(xué)附屬第五醫(yī)院骨一科;
【分類號(hào)】：R318.08;R683

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