本文選題：脊髓損傷　切入點：間充質(zhì)干細胞　出處：《安徽醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的觀察骨髓間充質(zhì)干細胞的條件培養(yǎng)基(BMSC-CM)局部移植對脊髓損傷修復(fù)的作用。方法采用改良的Allen裝置制作大鼠T9脊髓損傷模型。隨機分為對照組(脊髓損傷后局部微量注射生理鹽水10μl)和實驗組(脊髓損傷后局部注射BMSC-CM 10μl),兩組均采用BBB評分、斜板實驗法觀察大鼠運動功能恢復(fù)情況,蘇木精-伊紅(HE)染色觀察脊髓損傷處血管生長及空洞體積的改變情況。結(jié)果BMSC-CM局部移植組的大鼠BBB后肢功能評分、斜板的傾斜角度明顯高于對照組(P0.05),脊髓損傷處的空洞體積明顯小于對照組,血管直徑大于對照組。結(jié)論在徹底避免了BMSCs的直接和間接作用下用BMSC-CM能夠減小脊髓損傷處的空洞體積和促進血管生長,從而促進脊髓損傷后神經(jīng)功能的恢復(fù)。
[Abstract]:Objective to observe the effect of local transplantation of bone marrow mesenchymal stem cells (BMSCs) on the repair of spinal cord injury. Methods the model of T9 spinal cord injury in rats was made by modified Allen device. BBB score was used in both groups (BMSC-CM 10 渭 l after spinal cord injury) and experimental group (10 渭 l normal saline). The recovery of motor function and the changes of vascular growth and cavity volume in spinal cord injury were observed by hematoxylin and eosin (HEH) staining. Results the BBB hind limb function score of rats in BMSC-CM local transplantation group was observed. The inclined angle of the inclined plate was significantly higher than that of the control group (P 0.05), and the volume of the cavity in the spinal cord injury was obviously smaller than that in the control group. Conclusion under the direct and indirect action of BMSCs, BMSC-CM can reduce the volume of cavities and promote the growth of blood vessels in spinal cord injury, thus promoting the recovery of nerve function after spinal cord injury.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R651.2

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相關(guān)期刊論文 前1條

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本文編號：1658982