本文選題：鞘內(nèi)注射　切入點(diǎn)：右美托咪定　出處：《中國比較醫(yī)學(xué)雜志》2017年06期 　論文類型：期刊論文

【摘要】：目的分析右美托咪定鞘內(nèi)注射對于慢性神經(jīng)痛模型鼠的行為、疼痛程度脊髓背角蛋白激酶C(protein kinase C,即PKC)的影響,初步探討右美托咪定的鎮(zhèn)痛機(jī)制。方法將50只成功建立模型的大鼠隨機(jī)分為觀察組、模型組,另選取25只健康大鼠作為對照組,觀察組和模型組大鼠建立慢性坐骨神經(jīng)損傷模型,在建模后,觀察組應(yīng)用右美托咪定鞘內(nèi)注射干預(yù),模型組應(yīng)用生理鹽水注射,對照組不接受干預(yù),在建模前、給藥后3、5、7、14 d時分別進(jìn)行行為能力評價(累積評分法和運(yùn)動功能評價)和疼痛程度評價(機(jī)械性縮足反射法和熱縮足反射潛伏期法檢測痛閾值),同時進(jìn)行PKC免疫染色評分(免疫組化SABC法)、PKCmRNA檢測(RT-PCR法)和PKC蛋白(Western blot法)表達(dá),利用統(tǒng)計(jì)方法分析組間數(shù)據(jù)。結(jié)果 (1)建模前,各組大鼠行為累積評分、運(yùn)動功能評分、機(jī)械性縮足反射法(MWT)、熱縮足反射潛伏期法(TWL)差異無顯著性(P0.05),在建模后,模型組和觀察組大鼠累積評分和運(yùn)動功能評分顯著升高,MWT和TWL明顯下降,其中觀察組變化幅度顯著低于模型組,建模后,組間累積評分、運(yùn)動功能評分、MWT、TWL具有明顯差異(P0.05);(2)對照組PKC為陰性表達(dá),模型組PKC呈強(qiáng)陽性表達(dá),觀察組建模后初期PKC為強(qiáng)陽性,隨著治療時間延長,PKC表達(dá)強(qiáng)度降低,第14天時PKC為弱陽性表達(dá);(3)建模后,觀察組和模型組PKCmRNA和PKC蛋白表達(dá)水平顯著高于對照組(P0.05);隨著不斷給藥,觀察組PKCmRNA和PKC下降,在給藥14 d時,PKCmRNA和PKC接近對照組,建模后3 d起,在相同時間點(diǎn),觀察組PKC表達(dá)量顯著低于模型組(P0.05)。結(jié)論右美托咪定鞘內(nèi)注射能夠改善慢性神經(jīng)痛模型大鼠行為能力、降低疼痛程度,可能與其抑制脊髓背角蛋白激酶C的表達(dá)相關(guān)。
[Abstract]:Objective to analyze the effects of intrathecal injection of dexmetomidine on the behavior and pain degree of spinal dorsal keratin kinase (kinase) in rats with chronic neuralgia. Methods 50 successful rats were randomly divided into observation group, model group and 25 healthy rats as control group. The model of chronic sciatic nerve injury was established in the observation group and the model group. After the model was established, the observation group was treated with dexmetomidine intrathecal injection, the model group was injected with normal saline, the control group did not receive the intervention, before modeling, the model group was treated with dexmetomidine intrathecal injection. The behavioral ability (cumulative score and motor function evaluation) and pain degree (mechanical foot reflex and thermal foot reflex latency) were evaluated at 14 days after administration. PKC immunostaining was performed at the same time. The expression of color score (immunohistochemistry, SABC, RT-PCR) and PKC protein (Western blot) were observed. Results: before modeling, there were no significant differences in the scores of behavior accumulation, motor function, mechanical foot reflex (MWTT) and thermal foot reflex latency (TWL) in each group, but there was no significant difference between the two groups after modeling. The cumulative score and motor function score of rats in the model group and the observation group were significantly increased, and the changes of MWT and TWL in the observation group were significantly lower than those in the model group, and the cumulative score between the groups after modeling was significantly lower than that in the observation group. Motor function score (MWTT TWL) the PKC expression in the control group was negative, the PKC expression in the model group was strongly positive, and the PKC expression in the observation group was strongly positive at the initial stage after modeling, and decreased with the prolongation of the treatment time. The expression levels of PKCmRNA and PKC protein in observation group and model group were significantly higher than those in control group (P 0.05), and PKCmRNA and PKC in observation group decreased with continuous administration, and were similar to those in control group at day 14. From 3 days after modeling, the expression of PKC in the observation group was significantly lower than that in the model group at the same time point. Conclusion the intrathecal injection of dexmetomidine can improve the behavioral ability and reduce the degree of pain in the chronic neuralgia model rats. It may be related to the inhibition of the expression of dorsal keratin C in spinal cord.
【作者單位】： 海南醫(yī)學(xué)院第二附屬醫(yī)院麻醉科;廣州醫(yī)科大學(xué)附屬第四醫(yī)院麻醉科;
【分類號】：R-332;R614

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