發(fā)布時(shí)間：2018-03-19 08:35

  本文選題：醫(yī)用臭氧　切入點(diǎn)：腰椎間盤突出癥　出處：《山東大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：觀察不同濃度醫(yī)用臭氧對(duì)腰椎間盤突出癥患者髓核組織的結(jié)構(gòu)改變以及NO和IL-6表達(dá)的影響。方法：經(jīng)椎間孔鏡取出的腰椎間盤突出癥患者髓核16例,每例隨機(jī)分為5組：對(duì)照組(C組)不施加任何干預(yù),氧氣組(O2組)、醫(yī)用臭氧20μg/ml組(0320組)、醫(yī)用臭氧40μg/ml組(0340組)和醫(yī)用臭氧60μg/ml組(0360組)分別用O2、20μg/mlO 40μg/mlO3和60μg/mlO3干預(yù)20分鐘。體外培養(yǎng)72h后,HE染色觀察髓核組織結(jié)構(gòu)變化,分別采用硝酸酶還原法和酶聯(lián)免疫吸附法(ELISA)檢測(cè)培養(yǎng)液NO和IL-6含量,實(shí)時(shí)熒光定量PCR檢測(cè)iNOS mRNA和IL-6 mRNA表達(dá)。結(jié)果：1.髓核組織結(jié)構(gòu)改變：與對(duì)照組比較,O320組和O340組髓核組織的基質(zhì)成分改變和纖維沉積不明顯,0360組基質(zhì)成分減少,排列紊亂,膠原纖維增生。2.NO和IL-6表達(dá)變化：與C組比較,O2組NO和IL-6的表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P0.05)；與C組和02組比較,0320組和0340組NO和IL-6表達(dá)降低(P0.01),而0360組NO和IL-6表達(dá)升高(P0.05)。0320組和0340組之間NO和IL-6的表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P0.05)。3. iNOS mRNA和IL-6 mRNA表達(dá)變化：與C組比較,02組iNOS和IL-6mRNA的表達(dá)都無明顯差異(P0.05)。0320組和0340組iNOS mRNA表達(dá)明顯下降(P0.05),而0360組iNOS mRNA表達(dá)升高(P0.05)。同樣,與C組比較,0320組和0340組IL-6 mRNA表達(dá)也顯著下降(P0.05),而O360組IL-6 mRNA表達(dá)也明顯上升(P0.05)。結(jié)論：醫(yī)用臭氧具有降解病變髓核組織基質(zhì)成分和促纖維沉積作用。20μg/ml和40μg/ml醫(yī)用臭氧可抑制腰椎間盤突出癥患者髓核iNOS mRNA和IL-6 mRNA的表達(dá),減少NO合成,降低炎性反應(yīng)。60μg/ml醫(yī)用臭氧增加NO和iNOS mRNA以及IL-6和IL-6 mRNA的表達(dá)。
[Abstract]:Objective: to observe the effects of different concentrations of medical ozone on the structural changes of nucleus pulposus and the expression of no and IL-6 in the nucleus pulposus of patients with lumbar disc herniation. Methods: sixteen patients with lumbar disc herniation were treated by intervertebral foramen. Each case was randomly divided into 5 groups: control group C) without any intervention, O _ 2 / O _ 2 group, medical ozone 20 渭 g / ml group 0 320, medical ozone 40 渭 g / ml group 0 340) and medical ozone 60 渭 g / ml group 0 360) were treated with O _ 2O _ (20 渭 g / ml O _ (40) 渭 g / ml O _ (3) and 60 渭 g / ml O _ (3) for 20 minutes, respectively. After 72 hours of culture in vitro, the structure of nucleus pulposus was observed by HE staining. The contents of no and IL-6 in culture medium were detected by nitrate reduction method and enzyme linked immunosorbent assay (Elisa), respectively. Real time fluorescence quantitative PCR was used to detect the expression of iNOS mRNA and IL-6 mRNA. Results 1. Structural changes of nucleus pulposus: compared with the control group, the matrix composition and fiber deposition of nucleus pulposus in O320 group and O340 group were not obviously changed and the matrix components in group 0360 were decreased and disordered. 2. Changes of no and IL-6 expression: there was no significant difference in the expression of no and IL-6 between group C and group C, but the expression of no and IL-6 in group C and group 0340 decreased the expression of no and IL-6 in group C and group 02, while the expression of no and IL-6 in group 0360 increased the expression of no and IL-6 in group P 0.05.0320. There was no significant difference in the expression of no and IL-6 between groups 0 340 and 0 340. There was no significant difference in the expression of iNOS mRNA and IL-6mRNA between group 0 and group 0 340. There was no significant difference in the expression of iNOS and IL-6mRNA between group 2 and group C, but the expression of iNOS mRNA in group 0 340 and group 0 320 decreased significantly, while the expression of iNOS mRNA increased in group 0 360. Compared with group C, the expression of IL-6 mRNA in group C0320 and group 0340 also decreased significantly, while the expression of IL-6 mRNA in group O360 also increased significantly. Conclusion: medical ozone can degrade the matrix components of diseased nucleus pulposus and promote the deposition of fibers. 20 渭 g / ml and 40 渭 g / ml of medical ozone can be used to treat the lesion. To inhibit the expression of iNOS mRNA and IL-6 mRNA in nucleus pulposus of patients with lumbar disc herniation. The expression of no, iNOS mRNA, IL-6 and IL-6 mRNA was increased by reducing the synthesis of no and decreasing the inflammatory response. 60 渭 g / ml of medical ozone increased the expression of no, iNOS mRNA and IL-6 mRNA.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R614

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本文編號(hào)：1633485