發(fā)布時(shí)間：2018-03-16 02:28

  本文選題：羥基喜樹堿　切入點(diǎn)：關(guān)節(jié)瘢痕粘連　出處：《揚(yáng)州大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：膝關(guān)節(jié)術(shù)后,羥基喜樹堿可以通過抑制成纖維細(xì)胞增殖來減少關(guān)節(jié)粘連。但是,羥基喜樹堿預(yù)防膝關(guān)節(jié)粘連的最佳濃度仍然不清。故本研究擬探討羥基喜樹堿預(yù)防膝關(guān)節(jié)術(shù)后瘢痕粘連的最佳濃度。方法：將60只新西蘭大白兔隨機(jī)分為5組。手術(shù)去除股骨髁兩側(cè)的皮質(zhì)骨,范圍為10mm×10mm大小,暴露松質(zhì)骨。將含有不同濃度羥基喜樹堿(0.1 mg/ml,0.5 mg/ml,1.0 mg/ml,2.0 mg/ml)或生理鹽水的棉片作用于手術(shù)區(qū)域10分鐘。術(shù)后四周,采用大體評(píng)分、羥脯氨酸含量測(cè)定和組織學(xué)觀察判斷膝關(guān)節(jié)粘連程度。結(jié)果：所有動(dòng)物術(shù)后恢復(fù)良好,飲食及飲水量正常,未發(fā)現(xiàn)有實(shí)驗(yàn)動(dòng)物死亡、切口感染及皮膚壞死等現(xiàn)象。不同濃度組的羥基喜樹堿均可以減少術(shù)后膝關(guān)節(jié)粘連,1.0mg/ml, 2.0mg/l羥基喜樹堿組的膝關(guān)節(jié)粘連程度明顯低于其他各組,且在1.0mg/ml濃度組之前隨著藥物濃度的不斷增加,膝關(guān)節(jié)瘢痕粘連的程度越低,而1.0 mg/ml和2.0 mg/ml濃度組之間的瘢痕粘連程度沒有統(tǒng)計(jì)學(xué)差異。結(jié)論：局部應(yīng)用1.0 mg/ml羥基喜樹堿或許是預(yù)防兔膝關(guān)節(jié)術(shù)后關(guān)節(jié)粘連的最佳濃
[Abstract]:Objective: after knee surgery, hydroxycamptothecin can reduce adhesion by inhibiting fibroblast proliferation. The best concentration of hydroxycamptothecin for preventing adhesion of knee joint is still unclear. Therefore, this study aims to explore the best concentration of hydroxycamptothecin in preventing scar adhesion after knee joint operation. Methods: 60 New Zealand rabbits were randomly divided into 5 groups. Removal of cortical bone on both sides of femoral condyle, The size of 10 mm 脳 10 mm exposed cancellous bone. A cotton sheet containing 0.1 mg / ml of different concentrations of HCPT (0.5 mg / ml) or normal saline was applied to the surgical area for 10 minutes. Results: all the animals recovered well, their diet and drinking water were normal, and no experimental animals were found to die. Different concentrations of hydroxycamptothecin can reduce postoperative knee joint adhesion 1.0 mg / ml, 2.0 mg / l hydroxycamptothecin group is significantly lower than other groups, the degree of knee joint adhesion is significantly lower than other groups. And before 1.0 mg / ml concentration group, with the increasing of drug concentration, the degree of knee joint scar adhesion was lower. However, there was no significant difference in the degree of scar adhesion between 1.0 mg/ml and 2.0 mg/ml groups. Conclusion: local application of 1.0 mg/ml hydroxycamptothecin may be the best concentration for preventing articular adhesion after knee joint surgery in rabbits.
【學(xué)位授予單位】：揚(yáng)州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R687.4

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張幫慧;;羥基喜樹堿副作用的臨床觀察和護(hù)理[J];齊齊哈爾醫(yī)學(xué)院學(xué)報(bào);2001年03期

2 齊菲;羥基喜樹堿劑型的研究進(jìn)展[J];中國(guó)藥業(yè);2005年09期

3 陳U，

本文編號(hào)：1617950