本文選題：富氫水　切入點：丙泊酚　出處：《貴陽醫(yī)學(xué)院》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:1、研究富氫水對乳鼠離體腦片谷氨酸損傷后的保護(hù)作用,并探討其在乳鼠離體腦片損傷后保護(hù)作用的最佳濃度;2、研究富氫水(hydrogen-rich water)聯(lián)合丙泊酚(propofol)后處理對谷氨酸(L-Glutamic Acid,Glu)致乳鼠離體腦片損傷的保護(hù)作用,并探討其機制。方法:1、切取并培養(yǎng)出生7天的SD大鼠乳鼠離體腦片,隨機分為10個小組。實驗分兩個步驟進(jìn)行。第一步:探索富氫水最佳保護(hù)濃度,5個小組分別為:25μmol/l富氫水+谷氨酸損傷組(HL25+Glu組)、50μmol/l富氫水+谷氨酸損傷組(HL50+Glu組)、100μmol/l富氫水+谷氨酸損傷組(HL100+Glu組)、200μmol/l富氫水+谷氨酸損傷組(HL200+Glu組)、300μmol/l富氫水+谷氨酸損傷組(HL300+Glu組);第二步,探討富氫水聯(lián)合丙泊酚的保護(hù)作用,5個小組分別為:正常對照組,Glu損傷組(Glu損傷組),3 mg/L丙泊酚處理組(PL3+Glu處理組),100μmol/l富氫水后處理組(HL100+Glu處理組),富氫水丙泊酚聯(lián)合組(HL100+PL3+Glu處理組)。每組12例。將Glu損傷組、HL25+Glu組、HL50+Glu組、HL100+Glu組、HL200+Glu組、HL300+Glu組及PL3+Glu、HL100+Glu、HL100+PL3+Glu處理組均培養(yǎng)至第六天,用含1 mmol/L Glu的培養(yǎng)基損傷30min,建立缺血再灌注模型,損傷后將Glu損傷組移入正常的完全培養(yǎng)基,將HL25+Glu組、HL50+Glu組、HL100+Glu組、HL200+Glu組、HL300+Glu組及PL3+Glu、HL100+Glu、HL100+PL3+Glu處理組分別移入含相應(yīng)處理藥物的完全培養(yǎng)基中繼續(xù)培養(yǎng),每3小時換液一次,共8次,24小時。不同富氫水濃度組檢測尼氏小體數(shù)量,藥物處理組檢測蘇木素一伊紅(HE)染色,尼氏小體計數(shù)、乳酸脫氫酶(Lactate dehydrogenase LDH)釋放率、堿性成纖維生長因子(b FGF)數(shù)目的測定來比較各組腦片神經(jīng)細(xì)胞的損傷程度。結(jié)果:1、與富氫水其他濃度組相比,濃度為100μmol/l的富氫水后處理組的尼氏小體數(shù)量最多;2、與正常對照組相比,Glu損傷后的各組腦片中神經(jīng)細(xì)胞均受到一定程度的損傷,表現(xiàn)為細(xì)胞數(shù)量減少,細(xì)胞膜破碎,胞核皺縮,LDH釋放率、b FGF數(shù)量明顯增高;尼氏小體數(shù)量減少;3、與Glu損傷組相比,富氫水及丙泊酚后處理的各組,均能改善腦片中神經(jīng)細(xì)胞形態(tài)結(jié)構(gòu)受損的情況,細(xì)胞膜破損減輕,胞核較完整,均能使尼氏小體數(shù)量增加,LDH釋放率及b FGF數(shù)量降低,而兩者之間差異均無統(tǒng)計學(xué)意義;4、富氫水聯(lián)合丙泊酚后處理組的尼氏小體數(shù)量較丙泊酚藥物后處理組增加,LDH釋放率及b FGF數(shù)量均降低;與100μmol/l的富氫水后處理組相較,藥物聯(lián)合組的尼氏小體數(shù)量增加,而LDH釋放率及b FGF數(shù)量均增加。結(jié)論:1、不同濃度的富氫水對谷氨酸介導(dǎo)的乳鼠離體腦片損傷確有一定的保護(hù)作用,其中以100μmol/l為最佳;2、丙泊酚對乳鼠離體腦片谷氨酸損傷后的保護(hù)作用確切;3、富氫水聯(lián)合丙泊酚對缺血再灌注損傷的保護(hù)作用較單獨使用效果更好,其機制可能與減輕神經(jīng)膠質(zhì)細(xì)胞過度活化有關(guān)。
[Abstract]:Objective to study the protective effect of hydrogen rich water on glutamate injury in isolated brain slices of neonatal rats. To study the protective effect of propofol propofolate combined with hydro-rich water on the injury induced by L-Glutamic Acidic acid (GluA) in isolated rat brain slices, and to investigate the protective effect of the optimal concentration of Glutamic acid on the injury of isolated rat brain slices induced by hydrogen rich water and propofol (P < 0.05). Methods the isolated brain slices of SD rats born 7 days old were cut and cultured at 1: 1. The experiment was divided into 10 groups randomly. The experiment was divided into two steps. The first step was to explore the best protection concentration of hydrogen rich water. Five groups were divided into 5 groups: 1: 25 渭 mol/l hydrogen rich water glutamate injury group, HL25 Glu group, 50 渭 mol/l hydrogen rich water glutamate injury group, HL-50 Glu group, 100 渭 mol/l. HL-100 Glu group, HL-200 Glu group, HL-200 Glu group, HL-300 Glu group, HL-300 Glu group, HL-200 Glu group, HL-300 Glu group, HL-100 Glu group, HL-200 Glu group, HL-200 Glu group, HL-300 Glu group, HL-200 Glu group, HL-200 Glu group, HL-300 Glu group. To investigate the protective effect of hydrogen rich water combined with propofol, the five groups were: normal control group, Glu injury group, mg/L injury group, propofol treatment group, mg/L Glu treatment group, 100 渭 mol/l hydrogen rich water post-treatment group, HL-100 Glu treatment group, hydrogen rich water propofol group, and hydrogen rich water propofol group. 12 cases in each group were treated with HL-100 PL3 Glu. All of them were cultured to 6th days in Glu injury group, HL-25 Glu group, HL50 Glu group, HL100 Glu group, HL200 Glu group, HL300 Glu group and PL3 Glull-HL100 Glu-HL100 PL3 Glu group. The model of ischemia-reperfusion was established by using the medium containing 1 mmol/L Glu for 30 minutes. After the injury, the Glu injury group was transferred into the normal complete medium. HL25 Glu group, HL-50 Glu group, HL-100 Glu group, HL-200 Glu group, HL-300 Glu group and PL3 GluH100 Glutin-HL100 PL3 Glu group were cultured in complete culture medium containing corresponding drugs respectively, and the solution was changed every 3 hours. The number of Nissl bodies was detected in different concentrations of hydrogen rich water, the staining of hematoxylin-eosin (HEH), the count of Nissl bodies and the release rate of lactate dehydrogenase (Lactate dehydrogenase LDHs) were detected in the drug treatment group. The number of basic fibroblast growth factor (bFGFs) was measured to compare the degree of nerve cell damage in brain slices in each group. Results: 1, compared with other concentrations of hydrogen rich water, Compared with the normal control group, the number of Nissl corpuscles in the 100 渭 mol/l hydrogen-rich water treatment group was the highest, and the nerve cells in the brain slices were damaged to a certain extent, which showed that the number of cells decreased and the cell membrane was broken, compared with the normal control group. The release rate of LDH and the number of Nissl corpuscles were significantly increased, and the number of Nissl bodies was decreased. Compared with the Glu injury group, all groups treated with hydrogen rich water and propofol could improve the damage of neuronal morphology and cell membrane in brain slices, and the damage of cell membrane was alleviated in all the groups treated with hydrogen rich water and propofol. Nuclear integrity increased the release rate of FGF and the number of b FGF in Nissl corpuscles. However, there was no significant difference between the two groups. The number of Nissl bodies in the hydrogen-rich water combined with propofol post-treatment group increased the release rate of FGF and the number of b FGF in the propofol post-treatment group, which was significantly lower than that in the post-treatment group with 100 渭 mol/l hydrogen rich water. In the drug combination group, the number of Nissl bodies increased, while the release rate of LDH and the number of b FGF increased. Conclusion different concentrations of hydrogen rich water have protective effect on glutamate mediated brain injury in isolated rat brain slices. The protective effect of propofol on glutamate injury in isolated brain slices of neonatal rats was 100 渭 mol/l. The protective effect of hydrogen rich water combined with propofol on ischemia-reperfusion injury was better than that of using propofol alone, and the protective effect of propofol on ischemia reperfusion injury was better than that of propofol alone. The mechanism may be related to reducing the excessive activation of glial cells.
【學(xué)位授予單位】：貴陽醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R614

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