本文選題：成人增生性瘢痕　切入點：細胞因子　出處：《川北醫(yī)學(xué)院》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:通過免疫組織化學(xué)法(SP法)和酶聯(lián)免疫吸附測定分析(enzyme-linked immunosorbent assay,ELISA)來檢測轉(zhuǎn)化生長因子-β1(transforming growth factor beta1,TGF-β1)和表皮生長因子(epidermal growth factor,EGF)在成人增生性瘢痕中的表達,并進行統(tǒng)計學(xué)分析,探討TGF-β1和EGF在成人增生性瘢痕發(fā)生中可能的作用,為臨床防治提供一定的依據(jù)。方法:通過收集2014年4月至2014年8月期間臨床成人增生性瘢痕標(biāo)本共48例(排除使用過激素、放療、化療、感染等情況),并收集同期手術(shù)棄掉的成人正常皮膚組織標(biāo)本21例。用SP法定性和ELISA法定量檢測TGF-β1和EGF在成人增生性瘢痕和成人正常皮膚中的表達情況,用統(tǒng)計學(xué)軟件SPSS 13.0分析。結(jié)果： 1.TGF-β1在成人正常皮膚中表達陽性率為19.04%(4/21),在成人增生性瘢痕中的表達陽性率為54.17%(26/48);TGF-β1在成人正常皮膚中和成人增生性瘢痕中的表達水平比較具有統(tǒng)計學(xué)差異(P0.05);2.EGF在成人正常皮膚中表達陽性率為23.81%(5/21),在成人增生性瘢痕中的表達陽性率為60.42%(29/48);EGF在成人正常皮膚中和成人增生性瘢痕中的表達水平比較具有統(tǒng)計學(xué)差異(P0.05);3.TGF-β1和EGF在成人增生性瘢痕中的表達水平具有正相關(guān)性(SP時R=0.362,P=0.012;ELISA時R=0.330,P=0.017)。結(jié)論： 1.TGF-β1在成人增生性瘢痕中表達陽性率高。2.EGF在成人增生性瘢痕中表達陽性率高。3.TGF-β1和EGF在成人增生性瘢痕中的表達水平呈正相關(guān)。
[Abstract]:Objective: to detect the expression of transforming growth factor- 尾 _ 1transforming growth factor beta _ 1 (TGF- 尾 _ 1) and epidermal growth factor (EGF) in adult hypertrophic scar by immunohistochemical method (SP) and enzyme-linked immunosorbent assaysa (Elisa), and to analyze the expression of TGF- 尾 _ 1 and epidermal growth factor-EGFin in adult hypertrophic scar by enzyme-linked immunosorbent assay (Elisa), and to analyze the expression of TGF- 尾 _ 1 in adult hypertrophic scar. To explore the possible role of TGF- 尾 1 and EGF in the development of adult hypertrophic scar, and to provide some basis for clinical prevention and treatment. Methods: from April 2014 to August 2014, 48 clinical adult hypertrophic scar specimens were collected (excluding the use of hypertrophic hormone). The expression of TGF- 尾 1 and EGF in adult hypertrophic scar and adult normal skin was detected by SP assay and ELISA assay. Results: 1. The positive rate of TGF- 尾 1 expression in adult normal skin was 19.044 / 21, and in adult hypertrophic scar was 54.17% / 48% TGF- 尾 1 in adult normal skin and adult hypertrophic scar. 2. The positive rate of TGF- 尾 1 expression in adult normal skin and adult hypertrophic scar was higher than that in adult hypertrophic scar. 2. The positive rate of EGF expression in adult normal skin was 23.81%, and in adult hypertrophic scar was 60.42%. The positive rate of EGF in adult normal skin and adult hypertrophic scar was significantly higher than that in adult hypertrophic scar. 2. The positive rate of EGF in adult normal skin was 23.81% and that in adult hypertrophic scar was 60.42% / 48%. 2. The positive rate of EGF in adult normal skin and adult hypertrophic scar was significantly higher than that in adult hypertrophic scar. There is a positive correlation between the expression of TGF- 尾 1 and EGF in adult hypertrophic scar. Conclusion: 1.The positive rate of TGF- 尾 1 expression in adult hypertrophic scar is high. 2.EGF positive rate in adult hypertrophic scar. 3. TGF- 尾 1 and EGF in adult hypertrophic scar. 3. TGF- 尾 1 and EGF in adult hypertrophic scar. 3. TGF- 尾 1 and EGF in adult hypertrophic scar. 2. The positive rate of TGF- 尾 1 and EGF in adult hypertrophic scar. 3. TGF- 尾 1 and EGF in adult hypertrophic scar. 2. The positive rate of TGF- 尾 1 and EGF in adult hypertrophic scar. The expression level of hypertrophic scar was positively correlated.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R622

【參考文獻】

相關(guān)期刊論文 前10條

1 王延葉,李榮山;TGF-β1/Smad與腎臟間質(zhì)纖維化[J];國外醫(yī)學(xué).泌尿系統(tǒng)分冊;2005年06期

2 王少華;曹廣信;王燕華;呂建平;李記森;李健;;病理性瘢痕中結(jié)締組織生長因子的免疫組化研究[J];中國美容醫(yī)學(xué);2007年08期

3 閆貴春;裴銀輝;;瘢痕發(fā)生機制研究進展[J];中國美容醫(yī)學(xué);2010年08期

4 曾維惠;王倩倩;覃靜凈;耿松梅;徐磊;聶建軍;;ALA-PDT對兔耳增生性瘢痕抑制效應(yīng)及作用機制的初步研究[J];中國美容醫(yī)學(xué);2011年01期

5 李戰(zhàn);農(nóng)曉琳;李佳荃;樸智;唐黎黎;;青蒿琥酯抑制增生性瘢痕成纖維細胞Smad3表達的研究[J];中國美容醫(yī)學(xué);2014年04期

6 劉琰,陸樹良,王志勇,楊奕敏,喬亮,廖鎮(zhèn)江;EGF對大鼠深Ⅱ度燙傷創(chuàng)面成纖維細胞的影響[J];上海第二醫(yī)科大學(xué)學(xué)報;2002年02期

7 唐世杰,龐素芳;增生性瘢痕和瘢痕疙瘩的發(fā)病機制[J];國外醫(yī)學(xué)(生理、病理科學(xué)與臨床分冊);1999年05期

8 付小兵,程飚,盛志勇;創(chuàng)面愈合與瘢痕形成的分子學(xué)研究[J];中國臨床康復(fù);2002年04期

9 董祥林,馬少林;轉(zhuǎn)化生長因子β在纖維化疾病中的研究與應(yīng)用[J];中國臨床康復(fù);2004年35期

10 姜篤銀,付小兵,陳偉,孫同柱;血管生成因子及其受體過表達與瘢痕疙瘩侵襲性生長[J];中華整形外科雜志;2004年02期

，

本文編號：1583519