本文選題：吡咯烷二硫代氨基甲酯(PDTC)　切入點：頸椎間盤退變　出處：《中國藥理學(xué)通報》2017年10期 　論文類型：期刊論文

【摘要】：目的觀察吡咯烷二硫代氨基甲酯(PDTC)對大鼠頸椎動力失衡模型頸椎間盤組織形態(tài)及腫瘤壞死因子α(TNF-α)、基質(zhì)金屬蛋白酶9(MMP-9)表達的影響,探討PDTC抑制頸椎間盤退變作用及其機制。方法 54只SD大鼠隨機分為3組,切斷大鼠頸后部淺、深肌群構(gòu)建頸椎間盤退變動物模型。PDTC給藥組(A組):造模術(shù)后腹腔每日注射PDTC溶液。模型組(B組):造模術(shù)后腹腔每日注射生理鹽水。假手術(shù)組(C組):手術(shù)切開大鼠頸后部皮膚后立即縫合并每日腹腔注射生理鹽水。術(shù)后10、12、16周分批處死動物,獲取C5/6椎間盤組織,光鏡下觀察椎間盤形態(tài)改變,qPCR檢測椎間盤組織中TNF-αmRNA、MMP-9 mRNA表達;Western blot檢測椎間盤組織中TNF-α、MMP-9蛋白表達變化。結(jié)果 PDTC給藥組(A組)椎間盤結(jié)構(gòu)破壞減輕,纖維環(huán)結(jié)構(gòu)排列有序。模型組(B組)TNF-α、MMP-9基因表達量隨實驗時間的延長而增多,早期增速較快,后期逐漸緩慢,在12周達高峰,而蛋白表達量在術(shù)后10周即達到較高水平,與12周、16周時間點比較無明顯差異(P0.05)。各時間點PDTC給藥組TNF-αmRNA、MMP-9 mRNA及蛋白表達量較模型組(B組)明顯降低(P0.01),但明顯高于假手術(shù)組(P0.01)。結(jié)論 TNF-α、MMP-9是參與大鼠頸椎間盤退變過程的重要炎性因子,TNF-α、MMP-9可能通過NF-κB信號通路參與大鼠頸椎間盤退變,PDTC有望成為頸椎間盤退變的防治藥物。
[Abstract]:Objective to observe the effect of pyrrolidine dithiocarbamate (PDTCN) on the morphology of cervical intervertebral disc and the expression of matrix metalloproteinase 9 (MMP-9) in cervical disc of rats with cervical dynamic imbalance. To investigate the inhibitory effect of PDTC on cervical disc degeneration and its mechanism, 54 SD rats were randomly divided into 3 groups. Animal model of cervical intervertebral disc degeneration. Group A: intraperitoneal injection of PDTC solution daily after modeling. Group B: intraperitoneal injection of normal saline daily after modeling. The skin of the posterior part of the neck was sutured immediately and intraperitoneally injected with saline daily. The animals were executed in batches 10 weeks and 1216 weeks after operation. C5 / 6 intervertebral disc tissue was obtained. The morphological changes of intervertebral disc were observed under light microscope. The expression of TNF- 偽 mRNA-MMP-9 mRNA in intervertebral disc tissue was detected by qPCR and the expression of TNF- 偽 -mRNA-MMP-9 protein in intervertebral disc tissue was detected by Western blot. Results the structural damage of intervertebral disc was alleviated in group A (treated with PDTC). In group B, the expression of TNF- 偽 MMP-9 gene increased with the prolongation of experimental time. The expression of TNF- 偽 MMP-9 gene increased rapidly in the early stage, slowly in the later stage, and reached its peak at 12 weeks, while the protein expression reached a higher level at 10 weeks after operation. TNF- 偽 mRNA-MMP-9 mRNA and protein expression in PDTC group were significantly lower than those in model group B (P 0.01), but significantly higher than that in sham operation group (P 0.01). Conclusion TNF- 偽 MMP-9 is involved in cervical disc degeneration in rats. TNF- 偽 -MMP-9 may be involved in the degeneration of rat cervical intervertebral disc through NF- 魏 B signaling pathway, and PDTC may be a drug to prevent and treat the degeneration of cervical intervertebral disc.
【作者單位】： 江西省人民醫(yī)院骨二科;
【基金】：江西省科技支撐計劃資助項目(No 20122BBG70118,20151BBG70119)
【分類號】：R681.5

【相似文獻】

相關(guān)期刊論文 前10條

1 唐田;謝林;;頸椎間盤退變機制研究進展[J];中醫(yī)學(xué)報;2013年05期

2 王昆;謝林;;頸椎間盤退變機理和預(yù)防的研究進展[J];中醫(yī)正骨;2007年03期

3 王大鵬;付勤;陳洪亮;馬兆吉;楊禮慶;;人工椎間盤置換術(shù)治療頸椎間盤退變療效觀察[J];山東醫(yī)藥;2009年42期

4 許理忠,王擁軍,施杞;成人頸椎間盤退變機理研究[J];遼寧中醫(yī)雜志;2002年09期

5 劉越,趙曉勇,劉福成;頸椎間盤退變病因研究進展[J];中國矯形外科雜志;2004年19期

6 謝林;康然;施杞;侯鐵勝;沈培芝;徐宇;;骨密度與頸椎間盤退變關(guān)系的實驗研究[J];實用老年醫(yī)學(xué);2008年04期

7 陳濤平;陳玉敏;吳佳銘;;頸椎間盤退變致炎機制的實驗研究進展[J];現(xiàn)代中西醫(yī)結(jié)合雜志;2007年17期

8 陳艦艦;周臨東;謝林;唐田;;頸椎間盤退變動物模型的研究進展[J];中醫(yī)學(xué)報;2013年06期

9 張蕾;徐振華;林順新;賈吉光;陳森;鄭曉佐;;雌激素在大鼠頸椎間盤退變中的作用[J];武漢大學(xué)學(xué)報(醫(yī)學(xué)版);2009年06期

10 彭寶淦,賈連順,施杞,吳仕良;中性蛋白酶在頸椎間盤退變中的作用機制[J];第二軍醫(yī)大學(xué)學(xué)報;1999年09期

相關(guān)會議論文 前1條

1 應(yīng)航;詹紅生;陳立;井夫杰;熊愛民;;異常應(yīng)力環(huán)境導(dǎo)致頸椎間盤退變的實驗研究[A];中國生物醫(yī)學(xué)工程學(xué)會第六次會員代表大會暨學(xué)術(shù)會議論文摘要匯編[C];2004年

相關(guān)碩士學(xué)位論文 前3條

1 王大鵬;人工椎間盤置換術(shù)治療頸椎間盤退變療效觀察[D];中國醫(yī)科大學(xué);2010年

2 趙長清;細胞凋亡、細胞增殖與人頸椎間盤退變的關(guān)系[D];鄭州大學(xué);2005年

3 程勇軍;SOX9基因與人頸椎間盤退變相關(guān)性的初步研究[D];第二軍醫(yī)大學(xué);2012年

，

本文編號：1564839