本文關(guān)鍵詞： 膝骨關(guān)節(jié)炎 高遷移率族蛋白B- 關(guān)節(jié)滑液 嚴(yán)重程度　出處：《重慶醫(yī)科大學(xué)學(xué)報(bào)》2017年05期 　論文類型：期刊論文

【摘要】：目的:探討膝骨關(guān)節(jié)炎(knee osteoarthritis,KOA)患者關(guān)節(jié)液和血清中高遷移率族蛋白B-1(high mobility group box B1,HMGB1)的水平與骨關(guān)節(jié)炎嚴(yán)重程度的相關(guān)性。方法:選取72名住院接受關(guān)節(jié)鏡或全膝關(guān)節(jié)置換手術(shù)的KOA患者為實(shí)驗(yàn)組和20例因半月板或韌帶損傷患者為對照組(行關(guān)節(jié)鏡檢查后排除軟骨損傷),膝關(guān)節(jié)病變程度采用X線Kellgren-Lawrence(K-L)分級評估,骨關(guān)節(jié)炎的癥狀學(xué)采用西安大略和麥馬斯特大學(xué)骨關(guān)節(jié)炎指數(shù)(Western OntarioMc Master University Osteoarthritis Index,WOMAC)評分。采用雙抗夾心酶聯(lián)免疫吸附法(enzyme-linked immunosorbent assay,ELISA)檢測血清和關(guān)節(jié)液中HMGB1的濃度。比較2組之間和不同K-L分級患者之間血清和關(guān)節(jié)液HMGB1水平。用Spearman秩相關(guān)分析KOA患者血清和關(guān)節(jié)液HMGB1水平與K-L分級、WOMAC評分的相關(guān)性。結(jié)果:KOA患者關(guān)節(jié)液HMGB1水平高于對照組(t=12.171,P=0.000);KOA患者血清HMGB1水平與對照組比較差異無統(tǒng)計(jì)學(xué)意義(t=0.247,P=0.620);KOA患者關(guān)節(jié)液和血清HMGB1水平無相關(guān)性(r=0.021,P=0.863);KOA患者關(guān)節(jié)液HMGB1的水平與WOMAC總分以及各個(gè)亞評分(疼痛、僵硬和功能評分)呈正相關(guān),與X線K-L分級呈正相關(guān);血清HMGB1的水平與WOMAC總分和各個(gè)亞評分(疼痛、僵硬和功能評分)及X線K-L分級無統(tǒng)計(jì)學(xué)相關(guān)性。結(jié)論:KOA患者關(guān)節(jié)液HMGB1可能成為一種潛在的評估KOA癥狀嚴(yán)重程度的生物標(biāo)志物。
[Abstract]:Objective: to investigate the relationship between the high mobility group protein B-1high mobility group box B1HMGB1 and the severity of osteoarthritis in patients with knee osteoarthritis. Methods: 72 patients with knee osteoarthritis underwent arthroscopy or total knee arthroscopy. The patients with KOA undergoing replacement operation were treated with experimental group and 20 patients with meniscus or ligament injury as control group. The degree of knee joint lesion was evaluated by X-ray Kellgren Lawrenceen K-Ln, and cartilage injury was excluded after arthroscopy. The symptom of osteoarthritis was assessed by Western OntarioMc Master University Osteoarthritis Index (WMA). The serum and joint fluid (HMGB1) were detected by double antibody sandwich enzyme-linked immunosorbent assayamcine-linked immunosorbent assay (Elisa). The HMGB1 levels in serum and joint fluid were compared between the two groups. Serum and articular fluid (HMGB1) levels in patients with different K-L grades were analyzed by Spearman rank correlation. Results the serum and joint fluid HMGB1 levels in patients with KOA were higher than those in control group (P < 12.171). There was no significant difference between the serum HMGB1 level of the patients and the control group. There was no correlation between the level of HMGB1 in the articular fluid and the serum HMGB1 levels in the patients with Kota and in the control group. There was no correlation between the level of HMGB1 in the articular fluid of the patients and the total score of WOMAC and the subscore (pain) in the joint fluid of the patients. The level of serum HMGB1 was positively correlated with the total score of WOMAC and the subscale of WOMAC. Conclusion HMGB1 may be a potential biomarker for evaluating the severity of KOA symptoms.
【作者單位】： 宜賓市第一人民醫(yī)院骨二科;
【分類號】：R684.3

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