本文關(guān)鍵詞： 脂肪栓塞綜合征 骨髓脂肪 動物模型 兔　出處：《中國矯形外科雜志》2017年10期 　論文類型：期刊論文

【摘要】：[目的]探討骨折并發(fā)脂肪栓塞綜合征(FES)動物模型的建立方法和影響因素。[方法]32只新西蘭大白兔隨機分為模型組(A、B、C組)和對照組(D組),A、B、C組以0.2 ml/min經(jīng)股靜脈注入0.2、0.4、0.8 ml/kg骨髓脂肪,D組注入生理鹽水。在注射前(0 h)及注射后1、2、4、6、8 h,行血氣分析和血常規(guī)檢查。注射后8 h處死,觀察肺大體改變并行HE、油紅O染色。[結(jié)果](1)與對照組比較,模型組0.5 h內(nèi)均出現(xiàn)了呼吸急促等表現(xiàn),期間B組死亡3只(P0.05),C組均死亡(P0.01),A、D兩組無死亡;(2)與D組比較,A組動脈血氧分壓(PaO_2)最低值為(10.79±1.16)kPa(P0.05),B組為(6.78±0.93)kPa(P0.01);(3)注射8 h后,B組肺大體見暗紅色肝樣變,HE染色見脂肪空泡形成,油紅O染色見特異性橘紅色脂滴。[結(jié)論]B組兔在注射骨髓脂肪2 h后成功地建立了類似骨折并發(fā)FES的模型,成功率85%。
[Abstract]:[Objective] to explore the method and influencing factors of the animal model of fracture complicated with fat embolism syndrome (FESs). [Methods: Thirty-two New Zealand white rabbits were randomly divided into two groups: the model group (group A) and the control group (group D). Group D was injected with normal saline at 0 ml/kg. Blood gas analysis and routine examination were performed at 0 h before injection and 6 hours after injection. The rats were killed 8 hours after injection. The changes of lung were observed with HEY and oil red O staining. [Results: compared with the control group, the model group showed shortness of breath within 0.5 h. During the period, 3 rats died in group B and all died in group C (P 0.01). Group D had no death; (2) compared with group D, the lowest value of Pao _ 2 in group A was 10.79 鹵1.16kPaP0.05). In group B, it was 6.78 鹵0.93 KPA (P 0.01); 3) in group B, fat vacuoles were found in the lungs of group B after 8 hours of injection, and fat vacuoles were found in the lungs of group B with HE staining and specific orange fat drops with oil red O staining. [Conclusion: group B rabbits successfully established the model of similar fracture complicated with FES 2 h after injection of bone marrow fat, the success rate was 85%.
【作者單位】： 南方醫(yī)科大學(xué);中國人民解放軍武漢總醫(yī)院骨科;
【基金】：湖北省衛(wèi)計委重點科研項目(編號:WJ2015MA013)
【分類號】：R-332;R683
【正文快照】： 骨折并發(fā)脂肪栓塞綜合征(fat embolism syn-drome,FES)是指骨折后骨髓腔內(nèi)脂質(zhì)成分入血阻 塞于微循環(huán)而引起的一系列病理生理改變的臨床綜合征[1]。其特點是發(fā)病急、進展快,目前尚缺乏特異性治療藥物,嚴(yán)重威脅創(chuàng)傷骨折患者的生命。FES發(fā)病機制及防治方法多年來尚未取得突破性

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