本文關鍵詞： hIGF-1 aggrecanse-1 轉染 骨髓干細胞 微絲　出處：《青島大學》2016年碩士論文　論文類型：學位論文

【摘要】：目的探討h IGF-1(人胰島素生長樣因子-1)誘導和aggrecanse-1(多聚蛋白聚糖酶-1)沉默對人骨髓干細胞微絲結構的影響。方法用基因重組技術構建攜帶h IGF-I過表達和沉默aggrecanase-1-si RNA的慢病毒載體,用其轉染人骨髓干細胞,記為基因誘導組、基因沉默組、基因誘導沉默組,以不攜帶目的基因的慢病毒轉染人骨髓干細胞和未處理的人骨髓干細胞分別記為對照組1、對照組2,轉染7天后PCR檢測基因表達,同時激光共聚焦顯微鏡下觀察各組細胞的微絲結構,并用Image J檢測各組細胞的熒光強度。用SPSS軟件進行統(tǒng)計學分析。結果基因誘導沉默組的熒光強度(0.119±0.008)高于基因誘導組(0.081±0.001)和基因沉默組(0.080±0.003),基因誘導組和基因沉默組的熒光強度高于對照組1(0.065±0.003)和對照組2(0.066±0.005),差異有統(tǒng)計學意義(P0.01)。對照組1和對照組2的熒光強度差異無統(tǒng)計學意義(P0.05)。結論h IGF-1的誘導和aggrecanse-1的沉默均促進骨髓干細胞微絲結構的生成,誘導沉默雙向作用更顯著。
[Abstract]:Objective to investigate the induction of hIGF-1 (human insulin-like factor-1) and aggrecanse-1 (PC-1). Methods Lentivirus vector carrying h IGF-I overexpression and silencing aggrecanase-1-si RNA was constructed by gene recombination technique. Human bone marrow stem cells were transfected with them as gene induction group, gene silencing group and gene induced silencing group. Human bone marrow stem cells and untreated human bone marrow stem cells transfected with lentivirus without the target gene were recorded as control group 1 and control group 2 respectively. After 7 days of transfection, gene expression was detected by PCR. At the same time, the microfilament structure of each group was observed under confocal laser microscope. The fluorescence intensity of each group was detected by Image J and analyzed statistically by SPSS software. Results the fluorescence intensity of gene induced silencing group was 0.119 鹵0.008). It was higher than that of gene induction group (0.081 鹵0.001) and gene silencing group (0.080 鹵0.003). The fluorescence intensity of gene induced group and gene silencing group was higher than that of control group (1 0. 065 鹵0. 003) and control group (2. 066 鹵0. 005). There was no significant difference in fluorescence intensity between control group 1 and control group 2 (P0.05). Conclusion both h IGF-1 induction and aggrecanse-1 silencing can promote the formation of bone marrow stem cell microfilaments. The bidirectional effect of induced silencing is more significant.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R684

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