本文關(guān)鍵詞： hIGF-1 aggrecanse-1 轉(zhuǎn)染 骨髓干細(xì)胞 微絲　出處：《青島大學(xué)》2016年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的探討h IGF-1(人胰島素生長(zhǎng)樣因子-1)誘導(dǎo)和aggrecanse-1(多聚蛋白聚糖酶-1)沉默對(duì)人骨髓干細(xì)胞微絲結(jié)構(gòu)的影響。方法用基因重組技術(shù)構(gòu)建攜帶h IGF-I過(guò)表達(dá)和沉默aggrecanase-1-si RNA的慢病毒載體,用其轉(zhuǎn)染人骨髓干細(xì)胞,記為基因誘導(dǎo)組、基因沉默組、基因誘導(dǎo)沉默組,以不攜帶目的基因的慢病毒轉(zhuǎn)染人骨髓干細(xì)胞和未處理的人骨髓干細(xì)胞分別記為對(duì)照組1、對(duì)照組2,轉(zhuǎn)染7天后PCR檢測(cè)基因表達(dá),同時(shí)激光共聚焦顯微鏡下觀察各組細(xì)胞的微絲結(jié)構(gòu),并用Image J檢測(cè)各組細(xì)胞的熒光強(qiáng)度。用SPSS軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果基因誘導(dǎo)沉默組的熒光強(qiáng)度(0.119±0.008)高于基因誘導(dǎo)組(0.081±0.001)和基因沉默組(0.080±0.003),基因誘導(dǎo)組和基因沉默組的熒光強(qiáng)度高于對(duì)照組1(0.065±0.003)和對(duì)照組2(0.066±0.005),差異有統(tǒng)計(jì)學(xué)意義(P0.01)。對(duì)照組1和對(duì)照組2的熒光強(qiáng)度差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論h IGF-1的誘導(dǎo)和aggrecanse-1的沉默均促進(jìn)骨髓干細(xì)胞微絲結(jié)構(gòu)的生成,誘導(dǎo)沉默雙向作用更顯著。
[Abstract]:Objective to investigate the induction of hIGF-1 (human insulin-like factor-1) and aggrecanse-1 (PC-1). Methods Lentivirus vector carrying h IGF-I overexpression and silencing aggrecanase-1-si RNA was constructed by gene recombination technique. Human bone marrow stem cells were transfected with them as gene induction group, gene silencing group and gene induced silencing group. Human bone marrow stem cells and untreated human bone marrow stem cells transfected with lentivirus without the target gene were recorded as control group 1 and control group 2 respectively. After 7 days of transfection, gene expression was detected by PCR. At the same time, the microfilament structure of each group was observed under confocal laser microscope. The fluorescence intensity of each group was detected by Image J and analyzed statistically by SPSS software. Results the fluorescence intensity of gene induced silencing group was 0.119 鹵0.008). It was higher than that of gene induction group (0.081 鹵0.001) and gene silencing group (0.080 鹵0.003). The fluorescence intensity of gene induced group and gene silencing group was higher than that of control group (1 0. 065 鹵0. 003) and control group (2. 066 鹵0. 005). There was no significant difference in fluorescence intensity between control group 1 and control group 2 (P0.05). Conclusion both h IGF-1 induction and aggrecanse-1 silencing can promote the formation of bone marrow stem cell microfilaments. The bidirectional effect of induced silencing is more significant.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：R684

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 Jing-Bo Ji;Xue-Fu Li;Lei Liu;Guang-Zhong Wang;Xin-Feng Yan;;Effect of low intensity pulsed ultrasound on expression of TIMP-2 in serum and expression of mmp-13 in articular cartilage of rabbits with knee osteoarthritis[J];Asian Pacific Journal of Tropical Medicine;2015年12期

2 潘志敏;馬勇;程細(xì)高;;常用病毒載體的基礎(chǔ)研究及臨床應(yīng)用[J];中國(guó)生化藥物雜志;2011年05期

3 許澍洽;許揚(yáng)濱;;BMSCs構(gòu)建組織工程軟骨的研究進(jìn)展[J];中國(guó)修復(fù)重建外科雜志;2008年02期

4 胡煒;項(xiàng)舟;張學(xué)利;周海濤;楊志明;;BMSCs復(fù)合膠原材料三維誘導(dǎo)軟骨細(xì)胞的初步研究[J];中國(guó)修復(fù)重建外科雜志;2008年02期

5 戚孟春,胡靜,鄒淑娟,韓力赤,羅恩;大鼠骨髓間充質(zhì)干細(xì)胞和顱骨成骨細(xì)胞在張應(yīng)力下細(xì)胞骨架的改變[J];華西口腔醫(yī)學(xué)雜志;2005年02期

6 熊培穎,黃生高;細(xì)胞骨架與力信號(hào)傳導(dǎo)[J];國(guó)外醫(yī)學(xué).口腔醫(yī)學(xué)分冊(cè);2005年02期

，

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