本文關(guān)鍵詞： 膠原支架 血管內(nèi)皮生長因子 脊髓損傷 神經(jīng)再生　出處：《蘇州大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:本研究通過建立SD大鼠脊髓第九胸椎(T9)全切損傷模型,評價膠原支架(CS)聯(lián)合膠原結(jié)合區(qū)-血管內(nèi)皮生長因子(CBD-VEGF)移植對脊髓損傷的修復(fù)作用。方法:80只健康雄性6周齡SD大鼠,體重為215±15g,隨機(jī)分成4組:1.假手術(shù)組;2.對照組;3.CS移植組;4.CS/CBD-VEGF移植組。術(shù)后對各組大鼠每周進(jìn)行一次BBB評分,并在術(shù)后第4周、10周和12周末施行組織學(xué)檢查及免疫組化等檢查,在10周、12周末行雙側(cè)腓腸肌測重及Masson染色。結(jié)果:根據(jù)排除準(zhǔn)則,所有實驗對象手術(shù)成功,各實驗組無大鼠因脊髓橫斷不完全而淘汰,共計80只大鼠納入研究。在術(shù)后4周末CS/CBD-VEGF組的生存率明顯高于對照組與CS組,但總體趨勢無統(tǒng)計學(xué)意義。術(shù)后7周,CS/CBD-VEGF組與CS組的雙下肢BBB評分逐漸高于對照組(p≤0.05),CS組與對照組間評分差異則至10周后逐步出現(xiàn)(p≤0.05)。4周、10周及12周的免疫組化分析可見:CS/CBD-VEGF組anti-NF、anti-NSE及anti-CD34染色密度明顯比CS組與對照組高,anti-GFAP染色密度較后兩者低,說明脊髓損傷處神經(jīng)纖維及血管再生情況較CS組及對照組好,且有較少的瘢痕組織形成。12周末腓腸肌重量分析可見:CS/CBD-VEGF組雙側(cè)腓腸肌重量明顯比CS組與對照組高(p≤0.05),同組雙下肢間無明顯差異(p0.05);Masson三色染色腓腸肌密度及粗細(xì)較CS組及對照組高(p≤0.05),表明損傷雙下肢肌肉萎縮情況較后兩組輕。結(jié)論:大鼠脊髓全橫斷損傷后,CS/CBD-VEGF的植入能夠有效改善損傷處局部微環(huán)境,抑制瘢痕組織的形成,促進(jìn)血管及神經(jīng)纖維的再生與雙下肢功能的恢復(fù)。
[Abstract]:Objective: to establish a model of total spinal cord injury in Sprague-Dawley rats (Sprague-Dawley) 9th thoracic spine (T9). Objective: to evaluate the effect of collagen scaffold combined with vascular endothelial growth factor (CBD-VEGF) graft on the repair of spinal cord injury (sci). The body weight was 215 鹵15g, and was randomly divided into 4 groups: 1: 1. Sham-operated group; 2. Control group; 3. CS transplantation group; 4. CSP / CBD-VEGF transplantation group. The rats in each group were evaluated with BBB once a week after operation, and the histological and immunohistochemical examinations were performed at the 4th week and the 12th week after operation. Results: according to the rule of exclusion, all the subjects were successfully operated, and no rats in each experimental group were eliminated because of incomplete transection of spinal cord. A total of 80 rats were included in the study. The survival rate of CS/CBD-VEGF group was significantly higher than that of control group and CS group at the end of 4 weeks, but the overall trend was not statistically significant. The lower limb BBB score of CS/CBD-VEGF group and CS group was higher than that of control group (p 鈮，

本文編號：1471040