本文關(guān)鍵詞： 焦磷酸鈉 磷酸二氫銨 β-TCP煅燒骨骨塊 表征 細(xì)胞相容性　出處：《口腔醫(yī)學(xué)研究》2017年05期 　論文類型：期刊論文

【摘要】：目的:對比研究焦磷酸鈉(Na_4P_2O_7.10H_2O,NP)和磷酸二氫銨(NH_4H_2PO_4,MAP)制備的成年牛及胎牛β-TCP煅燒骨骨塊的表征及細(xì)胞相容性,以期制備出一種理化性能較好且生物安全性可靠的純相β-TCP煅燒骨骨塊。方法:以XRD衍射分析、EDX能譜分析、電鏡掃描、Micro-CT掃描及材料力學(xué)性能檢測研究煅燒骨骨塊的表征,以體外細(xì)胞毒性實驗及細(xì)胞與材料的復(fù)合實驗探究煅燒骨骨塊的細(xì)胞相容性。結(jié)果:NH_4H_2PO_4制備的兩種煅燒骨骨塊其β-TCP純度較高,Ca/P比值也均接近于1.47;胎牛煅燒骨骨塊的孔隙率、抗壓強(qiáng)度及抗彎曲強(qiáng)度均高于成年牛煅燒骨骨塊(P0.05);NH_4H_2PO_4制備的兩種煅燒骨骨塊細(xì)胞毒性均為0級,但胎牛煅燒骨骨塊上細(xì)胞的粘附與增殖速率高于成年牛煅燒骨骨塊。結(jié)論:NH_4H_2PO_4制備的胎牛β-TCP煅燒骨骨塊具有較好的理化性能及細(xì)胞相容性,較適用于非承重區(qū)骨缺損的修復(fù)。
[Abstract]:Objective: to compare sodium pyrophosphate with NaSP _ 4P _ 2O _ (7.10H _ 2O _ (NPN)) and NH _ 4H _ 2PO _ 4 (NH _ 4H _ 2PO _ 4) with ammonium dihydrogen phosphate (NH _ 4H _ 2PO _ 4). The characterization and cytocompatibility of 尾 -TCP calcined bone mass from adult cattle and fetal cattle prepared by MAPA. Objective: to prepare a pure 尾 -TCP calcined bone mass with good physical and chemical properties and reliable biological safety. Methods: edX analysis and scanning electron microscopy (SEM) were used to analyze the bone mass. The characterization of calcined bone mass was studied by Micro-CT scanning and testing of mechanical properties of materials. The cytotoxicity test in vitro and the compound experiment of cell and material were used to study the cytocompatibility of calcined bone mass. Results the purity of 尾 -TCP in the two kinds of calcined bone pieces prepared by 1% NH4H2PO4 was high. The Ca/P ratios were close to 1. 47; The porosity, compressive strength and bending strength of calcinated bone mass of fetal cattle were higher than that of adult calcinated bone mass (P0.05). The cytotoxicity of the two kinds of calcined bone mass prepared by NH_4H_2PO_4 was 0 grade. However, the rate of cell adhesion and proliferation in calcinated bone mass of fetal cattle was higher than that in adult calcinated bone mass. Fetal bovine 尾 -TCP calcined bone mass prepared by NH_4H_2PO_4 has good physicochemical properties and cytocompatibility. It is suitable for the repair of bone defect in non-load-bearing area.
【作者單位】： 蘭州大學(xué)土木工程與力學(xué)學(xué)院;蘭州大學(xué)口腔醫(yī)學(xué)院;西北民族大學(xué)甘肅省口腔疾病研究重點實驗室;
【基金】：國家自然科學(xué)基金(編號:31560159/C0309;31360124/C0309)
【分類號】：R318.08;R68
【正文快照】： 煅燒骨(true bone ceramic,TBC)多選用牛松質(zhì)骨為原料,因其保留了原天然松質(zhì)骨的連續(xù)多孔結(jié)構(gòu),且材料來源不受限制,經(jīng)濟(jì)成本低,易于消毒和運輸[1]而備受青睞。但煅燒骨在800℃時主要成分為HAP[2],其降解性較差。在TBC中加入焦磷酸鈉或磷酸銨二次高溫煅燒,可生成主要成分為β-

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