The objective of this study was to investigate the effect of a new combined micro/nanoscale implant surface feature on osteoblasts’ behaviors including cell morphology, adhesion, proliferation, differentiation, and mineralization in vitro. A new micro/nano-hybrid topography surface was fabricated on commercial pure titanium（Cp Ti） by a two-step sandblasted acid-etching and subsequent alkali-and heattreatment（SA-AH）. The conventional sandblasted/acid-etching（SA） treatment and alkali and heat（AH） ... 

【文章來源】：Journal of Wuhan University of Technology（Materials Science）. 2017,32(02)EISCI

【文章頁數(shù)】：10 頁

【文章目錄】：
1 Introduction
2 Experimental
    2.1 Sample preparation
    2.2 Surface characteristic by scanning electron microscopy
    2.3 Cell preparation and culture
    2.4 Rat osteoblasts attachment assay
    2.5 Immunofluorescence of cellular cytos-keletal arrangements
    2.6 Cell morphology observation
    2.7 Cell proliferations assay
    2.8 Real time quantitative polymerase chain reaction（q RT-PCR）assay
    2.9 Mineralization assay
    2.1 0 Statistical analysis
3 Results
    3.1 Surface characteristics of Ti discs
    3.2 Osteoblast adhesion assay
    3.3 Immunofluorescence of cellular cytoskeletal arrangements
    3.4 Cell proliferations assay
    3.5 Cell morphological observation
    3.6 Gene expression of osteoblast markers
    3.7 Mineralization assay
4 Discussion
5 Conclusions


【參考文獻】：
期刊論文
[1]Effects of nanotopography on stem cell phenotypes[J]. Rajeswari Ravichandran,Clarisse CH Ng,Casey K Chan,Michael Raghunath,Seeram Ramakrishna.  World Journal of Stem Cells. 2009(01)



本文編號：3130708