【摘要】：目的·制備不同載銀量的載銀羥基磷灰石(Ag-HAp),探討不同載銀量Ag-HAp的細胞相容性及其對體外培養(yǎng)大鼠骨髓間充質干細胞(BMSC)成骨活性的影響,確定適宜載銀量。方法·制備不同載銀量(x_(Ag)值)的Ag-HAp(x_(Ag)0.3、x_(Ag)0.5、x_(Ag)0.7)并對其進行表征。將材料分為對照組(x_(Ag)0)和實驗組(x_(Ag)0.3、x_(Ag)0.5、x_(Ag)0.7),檢測各組材料對BMSC的細胞相容性、成骨相關基因的表達和堿性磷酸酶分泌情況的影響。結果·材料表征結果證實合成產物為Ag-HAp,最終載銀量x_(Ag)0.7x_(Ag)0.5x_(Ag)0.3。細胞相容性實驗結果顯示:x_(Ag)0.3-HAp和x_(Ag)0.5-HAp為合格,x_(Ag)0.7-HAp有輕微毒性。Real-time PCR結果顯示:與對照組比較,x_(Ag)0.3組和x_(Ag)0.5組成骨相關基因表達上調,x_(Ag)0.7組成骨相關基因表達下調或無明顯改變。堿性磷酸酶定性定量實驗顯示:x_(Ag)0.3組和x_(Ag)0.5組堿性磷酸酶活性均高于對照組,x_(Ag)0.7組堿性磷酸酶活性低于對照組。結論·改變原材料中銀的加入量(即改變x_(Ag)值)可制得不同載銀量Ag-HAp,當x_(Ag)為0.3、0.5時,其細胞毒性實驗結果為合格,且可促進大鼠BMSC成骨相關基因的表達及堿性磷酸酶的分泌。
[Abstract]:Objective to prepare silver-loaded hydroxyapatite (Ag-HAp) with different silver loading, to investigate the cellular compatibility of different silver-loaded Ag-HAp and its effect on (BMSC) osteogenic activity of rat bone marrow mesenchymal stem cells cultured in vitro, and to determine the suitable silver loading. Methods Ag-HAp (x _ (Ag) 0.3, x _ (Ag) 0.5, x _ (Ag) 0.7) with different silver loading (x _ (Ag) value) was prepared and characterized. The materials were divided into control group (x _ (Ag) 0) and experimental group (x _ (Ag) 0.3, x _ (Ag) 0.5, x _ (Ag) 0.7). The cellular compatibility of each group to BMSC was detected. Effect of osteogenic related gene expression and alkaline phosphatase secretion. Results the results of material characterization confirmed that the synthesized product was the final silver carrying capacity of Ag-HAp, 0.7x( Ag) 0.5x( Ag) 0.3. The results of cell compatibility test showed that x _ (Ag) 0.3-HAp and x _ (Ag) 0.5-HAp were qualified and x _ (Ag) 0.7-HAp was slightly toxic. Real-time PCR results showed that compared with the control group, The expression of bone-related genes in x( Ag) group was up-regulated, while the expression of bone-related genes in x( Ag) group was down-regulated or had no significant change. The results of qualitative and quantitative experiments showed that the alkaline phosphatase activity in x( Ag) group and x( Ag) group was higher than that in control group, and the alkaline phosphatase activity in x( Ag) group was lower than that in control group. Conclusion different silver loading Ag-HAp, can be prepared by changing the amount of silver in raw materials (that is, changing the value of x _ (Ag). When the x _ (Ag) is 0.3,0.5, the cytotoxicity test results are qualified. It can promote the expression of osteogenic related genes and the secretion of alkaline phosphatase in rats with BMSC.
【作者單位】： 上海交通大學醫(yī)學院附屬第九人民醫(yī)院口腔修復科;
【基金】：上海市科學技術委員會項目(114119a3900,14411964200)~~
【分類號】：R318.08

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