【摘要】：本論文致力于構(gòu)建具有一定延展性、機(jī)械強(qiáng)度、高比表面積、良好生物相容性、優(yōu)異生物活性、可滲透的多孔結(jié)構(gòu)的鈦基鈦酸鹽納米線生物支架材料，探討其制備機(jī)理、性質(zhì)特征，探索其作為生物體植入裝置應(yīng)用的可能性。 論文首先綜述了生物支架材料最新研究進(jìn)展，，簡單介紹了生物支架材料研究領(lǐng)域所存在的關(guān)鍵問題。在總結(jié)已有研究成果的基礎(chǔ)上，提出本論文的研究設(shè)想和新型生物醫(yī)用支架材料的制備方案。 論文通過水熱合成法制備網(wǎng)格狀微孔結(jié)構(gòu)鈦基鈦酸鹽納米線基體材料，在類體液(SBF)體系中，采用電化學(xué)沉積技術(shù)，以上述基體材料為工作電極、鉑電極為對極、甘汞電極為參比電極，通過電流協(xié)助Ca2+、PO3-4與OH-相互作用反應(yīng)得到羥基磷酸鈣(HA)納米顆粒修飾的鈦基鈦酸鹽納米線支架材料。采用場發(fā)射掃描電子顯微鏡(FESEM)、透射電子顯微鏡(TEM)、X-射線衍射光譜(XRD)、傅立葉—衰減全反射紅外光譜(FTIR-ATR)等手段表征了此支架材料的結(jié)構(gòu)。結(jié)果表明，所制得的“項(xiàng)鏈珠模型”核殼結(jié)構(gòu)的HA納米顆粒修飾鈦基鈦酸鹽納米線支架材料與生物體自然骨結(jié)構(gòu)和功能相近，其中羥基磷酸鈣納米顆粒的長約150nm、寬約30nm，且結(jié)構(gòu)為六方晶系，此種材料在一定程度上達(dá)到了結(jié)構(gòu)功能化仿生的目的。通過體外細(xì)胞培養(yǎng)進(jìn)一步對比研究了人體造骨細(xì)胞MG63在HA納米顆粒修飾前后鈦基鈦酸鹽納米線支架材料表面上的吸附、接觸、生長和分化現(xiàn)象，得到HA納米顆粒的定向修飾有利于材料生物相容性和生物活性的進(jìn)一步提高。 論文以水熱合成技術(shù)得到的網(wǎng)格狀微孔結(jié)構(gòu)鈦基鈦酸鹽納米線基體材料為基礎(chǔ)，在Hummers、Offeman法得到2.0mg/mL的石墨烯納米片懸浮液體系中，采用電泳沉積技術(shù)，以上述基體材料為工作電極、鉑電極為對極，通過電流協(xié)助石墨烯納米片與鈦酸鹽納米線相互作用得到石墨烯納米片修飾的鈦基鈦酸鹽納米線支架材料。采用場發(fā)射掃描電子顯微鏡(FESEM)、X-射線衍射光譜(XRD)、拉曼光譜(Raman)、傅立葉—衰減全反射紅外光譜(FTIR-ATR)、熒光光譜(PL)、紫外—可見光譜(UV-vis)等手段表征了此支架材料的結(jié)構(gòu)。結(jié)果表明，隨著電泳沉積時間的延續(xù)，鈦酸鹽納米線基體材料上所修飾石墨烯納米片的形貌主要是從有規(guī)律的相互重疊到無規(guī)律的相互堆積的過程，且鈦基鈦酸鹽納米線的網(wǎng)格狀微孔結(jié)構(gòu)逐漸的遭到破壞。通過體外細(xì)胞培養(yǎng)進(jìn)一步研究了人體造骨細(xì)胞MG63在石墨烯納米片修飾前后的鈦基鈦酸鹽納米線支架材料表面上的吸附、接觸、生長和分化現(xiàn)象，得到石墨烯納米片的有規(guī)律的修飾有利于材料生物性能的改善。 最后，總結(jié)了論文所做的具體工作，并提出了論文中有待繼續(xù)解決的問題，為進(jìn)一步的研究工作明確了方向。
[Abstract]:The purpose of this paper is to construct titanium based titanate nanowires with porous structure with certain extensibility, mechanical strength, high specific surface area, good biocompatibility, excellent biological activity and permeable porous structure, and to explore the preparation mechanism of titanium based titanate nanowires with certain extensibility, mechanical strength, high specific surface area, good biocompatibility, excellent biological activity and permeable porous structure. To explore the possibility of its application as an organism implantation device. In this paper, the latest research progress of biological scaffold materials is reviewed, and the key problems in the research field of biological scaffold materials are briefly introduced. On the basis of summing up the existing research results, the research ideas of this paper and the preparation scheme of new biomedical scaffold materials are put forward. In this paper, the matrix material of lattice microporous titanium based titanate nanowires was prepared by hydrothermal synthesis. In the humoral (SBF) system, electrochemical deposition technology was used as the working electrode and platinum electrode was used. Calomel electrode was used as reference electrode to prepare titanium based titanate nanowires modified by calcium hydroxyphosphate (HA) nanoparticles by current-assisted interaction of Ca2, PO3-4 and OH-. The structure of the scaffold was characterized by field emission scanning electron microscope (FESEM), transmission electron microscope (TEM), X-ray diffraction spectrum (XRD), Fourier-attenuated total reflection infrared spectroscopy (FTIR-ATR). The results show that the structure and function of titanium based titanate nanowires modified by HA nanoparticles with "necklace bead model" core-shell structure are similar to those of natural bone, in which calcium hydroxyphosphate nanoparticles are about 150 nm long and 30 nm wide. And the structure is hexagonal system, which achieves the purpose of structural functionalization bionic to a certain extent. The adsorption, contact, growth and differentiation of human osteoblasts MG63 on the surface of titanium based titanate nanowires before and after HA nanoparticles modification were further studied by cell culture in vitro. The directional modification of HA nanoparticles is beneficial to the further improvement of biocompatibility and biological activity of the materials. In this paper, based on the matrix material of reticulated microporous titanium based titanate nanowires obtained by hydrothermal synthesis, the graphene nanoparticles suspension system of 2.0mg/mL was obtained by Hummers, offemaner method, and the electrodeposition technique was used. Using the matrix material as working electrode and platinum electrode as opposite electrode, graphene nanowires modified titanium based titanate nanowires were prepared by the interaction of graphene nanowires with titanate nanowires. Field emission scanning electron microscope (FESEM), X-ray diffraction spectrum (XRD), Raman spectrum (Raman), Fourier-attenuated total reflection infrared spectrum (FTIR-ATR), fluorescence spectrum (PL), The structure of the scaffold was characterized by UV-vis spectroscopy (UV-vis). The results show that the morphology of graphene nanoparticles modified on titanate nanowires is mainly from regular overlap to irregular stacking with the prolongation of electrophoresis deposition time. The meshed microporous structure of titanium-based titanate nanowires was gradually destroyed. The adsorption, contact, growth and differentiation of human osteoblasts MG63 on the surface of titanium based titanate nanowires before and after graphene nanoparticles modification were further studied by cell culture in vitro. The regular modification of graphene nanoparticles is beneficial to the improvement of biological properties of graphene nanoparticles. Finally, the specific work of the paper is summarized, and the problems to be solved in the paper are put forward, which clarifies the direction of further research work.
【學(xué)位授予單位】：浙江理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：TB383.1;R318.08

【共引文獻(xiàn)】

相關(guān)期刊論文 前1條

1 Lili Tan;Xiaoming Yu;Peng Wan;Ke Yang;;Biodegradable Materials for Bone Repairs:A Review[J];Journal of Materials Science & Technology;2013年06期

相關(guān)博士學(xué)位論文 前1條

1 范興平;生物活性梯度多孔鈦的制備及生物學(xué)評價[D];西南交通大學(xué);2012年

本文編號：2482024