發(fā)布時間：2019-04-12 19:28

【摘要】：背景:周圍神經缺損的治療在再生醫(yī)學領域仍然具有挑戰(zhàn)性,支架材料的應用被認為是有前途的探索方向之一,理想的支架材料應是安全、耐用和缺乏抗原性的。目的:探討制備一種新型的組織工程化改性膠原-殼聚糖支架,并探索其在大鼠橫斷坐骨神經模型中用作橋梁修復神經損傷的可能。方法:先制備出膠原-殼聚糖復合支架,然后用1-乙基-3-(3-二甲基氨基丙基)-碳化二亞胺,N-羥基琥珀酰亞胺對支架進行化學改性即交聯(lián)。接著將神經干細胞種植于支架上,之后連同支架-種子細胞在體內植入坐骨神經缺損部位。結果與結論:制得的變性交聯(lián)膠原-殼聚糖復合支架具有三維立體網狀結構。將支架-種子細胞移植4周后,可在損傷的坐骨神經中發(fā)現(xiàn)S-100神經絲蛋白200表達。結果表明這種組織工程化改性交聯(lián)膠原-殼聚糖復合支架具有良好的細胞相容性。
[Abstract]:Background: the treatment of peripheral nerve defects is still challenging in regenerative medicine. The application of stent materials is considered to be one of the promising exploration directions. The ideal scaffold materials should be safe, durable and lack of antigenicity. Aim: to study the preparation of a new type of tissue engineering modified collagen chitosan scaffold and explore the possibility of using it as a bridge to repair nerve injury in rat sciatic nerve model. Methods: collagen-chitosan composite scaffolds were prepared, and then the scaffolds were chemically modified with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide. Neural stem cells were then implanted on the scaffold, and then together with the scaffold-seed cells, the sciatic nerve defect was implanted into the body. Results & conclusion: the modified cross-linked collagen-chitosan composite scaffolds have three-dimensional reticular structure. Four weeks after transplantation of scaffold-seeded cells, the expression of neurofilament 200 was found in injured sciatic nerve. 4 weeks after transplantation, the expression of neurofilament 200 was found in the injured sciatic nerve. The results showed that the cross-linked collagen-chitosan composite scaffolds with tissue engineering had good cellular compatibility.
【作者單位】： 湖北醫(yī)藥學院第一臨床學院;湖北醫(yī)藥學院麻醉學研究所;湖北醫(yī)藥學院附屬太和醫(yī)院麻醉科;湖北醫(yī)藥學院基礎醫(yī)學研究所;
【基金】：國家教育部2012年度國家級大學生創(chuàng)新創(chuàng)業(yè)訓練計劃項目(201210929004) 國家教育部2016年度國家級大學生創(chuàng)新創(chuàng)業(yè)訓練計劃項目(201610929005) 十堰市科學技術研究與開發(fā)項目(14Y13) 2016年度湖北醫(yī)藥學院教學研究項目(2016027) 2016年度湖北醫(yī)藥學院附屬太和醫(yī)院科學研究基金(2016JJXM001) 湖北醫(yī)藥學院高層次人才科研啟動金(2011QDZR-1,2011QDZR-2) 湖北醫(yī)藥學院2015年度“十二五規(guī)劃”學科建設學科英才成長資助項目(2015)~~
【分類號】：R318.08

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1 陸江;陳絢麗;唐核心;佘納;朱W，

本文編號：2457318