【摘要】：背景:周?chē)窠?jīng)缺損的治療在再生醫(yī)學(xué)領(lǐng)域仍然具有挑戰(zhàn)性,支架材料的應(yīng)用被認(rèn)為是有前途的探索方向之一,理想的支架材料應(yīng)是安全、耐用和缺乏抗原性的。目的:探討制備一種新型的組織工程化改性膠原-殼聚糖支架,并探索其在大鼠橫斷坐骨神經(jīng)模型中用作橋梁修復(fù)神經(jīng)損傷的可能。方法:先制備出膠原-殼聚糖復(fù)合支架,然后用1-乙基-3-(3-二甲基氨基丙基)-碳化二亞胺,N-羥基琥珀酰亞胺對(duì)支架進(jìn)行化學(xué)改性即交聯(lián)。接著將神經(jīng)干細(xì)胞種植于支架上,之后連同支架-種子細(xì)胞在體內(nèi)植入坐骨神經(jīng)缺損部位。結(jié)果與結(jié)論:制得的變性交聯(lián)膠原-殼聚糖復(fù)合支架具有三維立體網(wǎng)狀結(jié)構(gòu)。將支架-種子細(xì)胞移植4周后,可在損傷的坐骨神經(jīng)中發(fā)現(xiàn)S-100神經(jīng)絲蛋白200表達(dá)。結(jié)果表明這種組織工程化改性交聯(lián)膠原-殼聚糖復(fù)合支架具有良好的細(xì)胞相容性。
[Abstract]:Background: the treatment of peripheral nerve defects is still challenging in regenerative medicine. The application of stent materials is considered to be one of the promising exploration directions. The ideal scaffold materials should be safe, durable and lack of antigenicity. Aim: to study the preparation of a new type of tissue engineering modified collagen chitosan scaffold and explore the possibility of using it as a bridge to repair nerve injury in rat sciatic nerve model. Methods: collagen-chitosan composite scaffolds were prepared, and then the scaffolds were chemically modified with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide. Neural stem cells were then implanted on the scaffold, and then together with the scaffold-seed cells, the sciatic nerve defect was implanted into the body. Results & conclusion: the modified cross-linked collagen-chitosan composite scaffolds have three-dimensional reticular structure. Four weeks after transplantation of scaffold-seeded cells, the expression of neurofilament 200 was found in injured sciatic nerve. 4 weeks after transplantation, the expression of neurofilament 200 was found in the injured sciatic nerve. The results showed that the cross-linked collagen-chitosan composite scaffolds with tissue engineering had good cellular compatibility.
【作者單位】： 湖北醫(yī)藥學(xué)院第一臨床學(xué)院;湖北醫(yī)藥學(xué)院麻醉學(xué)研究所;湖北醫(yī)藥學(xué)院附屬太和醫(yī)院麻醉科;湖北醫(yī)藥學(xué)院基礎(chǔ)醫(yī)學(xué)研究所;
【基金】：國(guó)家教育部2012年度國(guó)家級(jí)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(201210929004) 國(guó)家教育部2016年度國(guó)家級(jí)大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計(jì)劃項(xiàng)目(201610929005) 十堰市科學(xué)技術(shù)研究與開(kāi)發(fā)項(xiàng)目(14Y13) 2016年度湖北醫(yī)藥學(xué)院教學(xué)研究項(xiàng)目(2016027) 2016年度湖北醫(yī)藥學(xué)院附屬太和醫(yī)院科學(xué)研究基金(2016JJXM001) 湖北醫(yī)藥學(xué)院高層次人才科研啟動(dòng)金(2011QDZR-1,2011QDZR-2) 湖北醫(yī)藥學(xué)院2015年度“十二五規(guī)劃”學(xué)科建設(shè)學(xué)科英才成長(zhǎng)資助項(xiàng)目(2015)~~
【分類(lèi)號(hào)】：R318.08

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 Feng Yan;Wei Yue;Yue-lin Zhang;Guo-chao Mao;Ke Gao;Zhen-xing Zuo;Ya-jing Zhang;Hui Lu;;Chitosan-collagen porous scaffold and bone marrow mesenchymal stem cell transplantation for ischemic stroke[J];Neural Regeneration Research;2015年09期

2 Qing Li;Jiang Lu;Xianyu Wang;;Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells[J];Neural Regeneration Research;2014年22期

3 ;Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro[J];Neural Regeneration Research;2012年22期

4 ;Distribution and localization of fibroblast growth factor-8 in rat brain and nerve cells during neural stem/progenitor cell differentiation[J];Neural Regeneration Research;2012年19期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陸江;陳絢麗;唐核心;佘納;朱W，

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