【摘要】：視覺(jué)假體作為失明患者視力最有潛力的修復(fù)方案之一,現(xiàn)已是國(guó)內(nèi)外學(xué)者研究的熱點(diǎn)。視覺(jué)假體中視網(wǎng)膜假體對(duì)外層感光細(xì)胞受損引起的視網(wǎng)膜退行性病變的功能性修復(fù)已取得了一定成果。視網(wǎng)膜微電極陣列作為視網(wǎng)膜假體關(guān)鍵性部件,通過(guò)刺激視網(wǎng)膜內(nèi)層細(xì)胞產(chǎn)生電信號(hào),經(jīng)視神經(jīng)傳遞至大腦皮層,形成視覺(jué)。其電學(xué)特性和體內(nèi)植入的可行性是保證視網(wǎng)膜假體安全穩(wěn)定工作的前提條件。本研究針對(duì)視網(wǎng)膜微電極陣列的結(jié)構(gòu)特點(diǎn)和植入要求,設(shè)計(jì)制作以Parylene-C為襯底,金作為電極材料,直徑為200μm的圓形柔性視網(wǎng)膜微電極,用于視網(wǎng)膜表層刺激,并通過(guò)體外電化學(xué)測(cè)試和眼內(nèi)植入可行性測(cè)試對(duì)其性能進(jìn)行評(píng)估。視網(wǎng)膜微電極表面形貌在掃描電鏡下呈現(xiàn)出良好的粗糙度,有助于電極呈現(xiàn)較低的電化學(xué)阻抗和較高的電荷注入能力。微電極陣列進(jìn)行體外電化學(xué)測(cè)試采用三電極測(cè)試系統(tǒng),搭建眼內(nèi)仿真環(huán)境進(jìn)行測(cè)試,實(shí)驗(yàn)方法包括電化學(xué)阻抗譜、循環(huán)伏安法、瞬時(shí)電壓法,分別測(cè)試并計(jì)算得出微電極電化學(xué)阻抗、電荷儲(chǔ)存能力和電荷注入能力。本研究測(cè)試得到微電極的電化學(xué)阻抗在1KHz時(shí)的阻抗為36.54±0.88 kΩ,相位角為-73.52±1.3°,電荷儲(chǔ)存能力為103.33±15μC/cm2,電荷密度為22.3μC/cm2。視網(wǎng)膜微電極成功植入大鼠眼內(nèi),自然彎曲,貼合于視網(wǎng)膜上,展現(xiàn)出了良好的柔韌性。結(jié)果表明,本文采用的微電極,在降低成本、簡(jiǎn)化工藝的基礎(chǔ)上,無(wú)論柔韌性還是電學(xué)特性都符合視網(wǎng)膜植入電極的要求,可用作動(dòng)物實(shí)驗(yàn)的視網(wǎng)膜植入電極。
[Abstract]:As one of the most potential visual restoration schemes for blind patients, visual prosthesis has become a hot research topic both at home and abroad. Some achievements have been achieved in the functional repair of retinal degeneration caused by photoreceptor cell damage in visual prosthesis. Retinal microelectrode array, as a key component of retinal prosthesis, produces electrical signals through optic nerve to form vision by stimulating inner layer cells of retina. Its electrical properties and the feasibility of implantation in vivo are the prerequisite to ensure the safety and stability of the retinal prosthesis. According to the structural characteristics and implantation requirements of retinal microelectrode array, a flexible circular retinal microelectrode with Parylene-C as substrate, gold as electrode material and 200 渭 m in diameter was designed and fabricated for retinal surface stimulation. The performance was evaluated by in vitro electrochemical test and intraocular implantation feasibility test. The surface morphology of retinal microelectrode shows good roughness under scanning electron microscope, which is helpful for the electrode to show lower electrochemical impedance and higher charge injection ability. The microelectrode array was tested in vitro by a three-electrode test system. The experimental methods included electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and transient voltage method (IVA), and an in-eye simulation environment was set up for the test. The electrochemical impedance, charge storage capacity and charge injection capacity of the microelectrode were measured and calculated respectively. The electrochemical impedance of the microelectrode at 1KHz is 36.54 鹵0.88 k 惟, the phase angle is-73.52 鹵1.3 擄, and the charge storage capacity is 103.33 鹵15 渭 C / cm2, charge density 22.3 渭 C / cm2.. The retinal microelectrode was successfully implanted into the eyes of the rats, naturally bent, attached to the retina, showing good flexibility. The results show that the microelectrode used in this paper can be used as an implanted electrode in animal experiments on the basis of reducing the cost and simplifying the process. Both the flexibility and the electrical properties of the microelectrode can meet the requirements of the retinal implantation electrode.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R318.18

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本文編號(hào)：2457004