【摘要】：皮膚是人體的重要組成部分,它與我們的體溫控制,人體免疫力以及人體與外界的傳感等重要功能息息相關。創(chuàng)傷是我們不可忽視的一類疾病,傷口敷料一直是生活以及醫(yī)藥領域的必需品。傳統(tǒng)敷料已不再滿足人們的要求,所以近年來,可降解性合成聚合物納米纖維敷料成為大家研究的重點。聚乳酸(PLA)有著難以比擬的優(yōu)良生物降解性、生物相容性和一定的強度,廣泛應用于生物醫(yī)藥領域。近來,有關石墨烯的抗菌性以及其細胞毒性的研究很多,結果表明石墨烯具有很好的抑菌效果且對細胞無毒性,同時氧化石墨烯有著很好的親水性能,這為石墨烯在生物醫(yī)藥領域的應用提供了堅實的理論基礎。目前為止,尚未發(fā)現(xiàn)有關將石墨烯加入聚乳酸進行靜電紡絲,應用于生物傷口敷料的相關報道。本論文采用靜電紡絲的方法制備出石墨烯改性聚乳酸(PLA)納米纖維膜,探究石墨烯對納米纖維膜性能的影響,探索其在生物傷口敷料應用潛力。首先,我們用層間催化解離法(Interlayer catalytic exfoliation,ICE)制備石墨烯,并對石墨烯進行SEM、TEM、FT-IR、Raman、XRD等測試。測試結果表明石墨烯具有較大的尺寸,而且厚度小于2nm。最終得到的石墨烯幾乎沒有缺陷,保留了其原有的完整基本晶面。接著,我們對純的PLA進行靜電紡絲工藝的探討,得出如下結論:環(huán)境參數(shù):溫度為25℃,相對濕度為25%;紡絲液濃度;15%;過程工藝參數(shù):電壓為15kV喂料速率為0.5ml/h,針頭到收集裝置的水平距離為15cm;使用錫箔紙為最終收集裝置。運用這一工藝參數(shù)紡制了石墨烯改性納米纖維膜,對單根纖維進行了SEM以及TEM測試,結果表明石墨烯被包裹在纖維內部。對納米纖維膜進行了FT-IR、Raman以及XPS測試,再次表明膜中含有石墨烯。最后,我們對復合膜的抗菌性能進行了測試。先對石墨烯及氧化石墨烯進行了抑菌率測試,結果發(fā)現(xiàn)石墨烯幾乎不抗菌,而氧化石墨烯的抑菌率大于90%。然后分別對摻雜石墨烯與氧化石墨烯的靜電紡絲膜進行抑菌圈實驗,結果發(fā)現(xiàn)摻雜石墨烯的靜電紡絲膜沒有出現(xiàn)抑菌環(huán),而摻雜氧化石墨烯的靜電紡絲膜出現(xiàn)了一小圈的抑菌環(huán),但是很小,可能是由于膜中氧化石墨烯含量太少導致的。為將其應用在敷料方面,需要對其進行更加深入的探索研究。
[Abstract]:Skin is an important part of the human body, it is closely related to our temperature control, human immunity and human body and external sensors and other important functions. Trauma is a kind of disease that we can't ignore. Wound dressing has always been a necessity of life and medicine. Traditional dressings no longer meet the needs of people, so in recent years, degradable synthesis of polymer nanofiber dressings has become the focus of research. Poly (lactic acid) (PLA) is widely used in biomedicine because of its incomparable biodegradability, biocompatibility and certain strength. Recently, there have been a lot of researches about the antibacterial activity and cytotoxicity of graphene. The results show that graphene has good bacteriostatic effect and no toxicity to cells, and that graphene oxide has good hydrophilicity, and that graphene has good bacteriostatic effect and no toxicity to cells. This provides a solid theoretical basis for the application of graphene in the field of biomedicine. So far, it has not been reported that graphene was added to polylactic acid for electrospinning and applied to biological wound dressing. In this paper, graphene-modified polylactic acid (PLA) nano-fiber membrane was prepared by electrospinning, and the effect of graphene on the performance of nano-fiber membrane was investigated, and the potential application of graphene in bio-wound dressing was explored. Firstly, we prepared graphene by interlayer catalytic dissociation method (Interlayer catalytic exfoliation,ICE), and tested graphene by SEM,TEM,FT-IR,Raman,XRD and so on. The results show that graphene has a large size and a thickness of less than 2 nm. The resulting graphene has almost no defects and retains its original basic crystal plane. Then, we discuss the electrospinning process of pure PLA and draw the following conclusions: the temperature is 25 鈩，

本文編號：2439313