【摘要】：目的:聚β-羥基丁酸酯(Polyβ-hydroxybutyrate,PHB)是一種由可以由多種細(xì)菌發(fā)酵得到的脂肪族聚酯,具有良好的生物相容性、生物降解性和壓電性等特點(diǎn)。本文旨在檢測(cè)聚β-羥基丁酸酯聚合物生物彈體部分物理性能以及研究該生物彈體與L-929細(xì)胞的生物相容性。方法:本研究首先對(duì)聚β-羥基丁酸酯聚乙二醇(PHB-PEG)共聚物進(jìn)行紅外光譜檢測(cè),核磁共振圖譜分析,示差掃描量熱分析,分析該物質(zhì)物理特性。其次在高溫、常溫情況下,我們將L-929細(xì)胞分別放置于聚β-羥基丁酸酯聚乙二醇聚合物薄膜、聚氯乙烯(PVC)薄膜上培養(yǎng),觀察第二、四、六天L-929細(xì)胞存活情況。結(jié)果:PHB-PEG共聚物表征檢測(cè)發(fā)現(xiàn):PHB-PEG紅外測(cè)試結(jié)果證實(shí)PHB-PEG之間鏈接存在;磁共振圖譜為PHB-PEG共聚物結(jié)構(gòu)提供了進(jìn)一步的證明;差示掃描量熱顯示PHB-PEG不同相之間的轉(zhuǎn)變溫度比PHB有所降低。L-929細(xì)胞與PHB-PEG共存實(shí)驗(yàn)顯示:高溫、常溫等環(huán)境條件下,PHB-PEG環(huán)境組細(xì)胞生長(zhǎng)狀況良好,較致密,有些細(xì)胞突開(kāi)始伸展,呈梭狀;而PV環(huán)境組第二天開(kāi)始出現(xiàn)細(xì)胞固縮,死亡。在高溫、常溫環(huán)境中,L-929細(xì)胞生長(zhǎng)情況在PHB組與陰性對(duì)照NaCl組間無(wú)顯著差異(p0.05);在PHB組與陽(yáng)性對(duì)照組PVC組間有顯著差異(p0.01);在陽(yáng)性對(duì)照PVC組與陰性對(duì)照NaCl組間有顯著差異(p0.01)。同組不同時(shí)間點(diǎn)的AO值采用協(xié)方差檢驗(yàn),得出陰性對(duì)照NaCl組和PHB-PEG組中L-929細(xì)胞生長(zhǎng)趨勢(shì)一致,陽(yáng)性對(duì)照組L-929細(xì)胞生長(zhǎng)呈被抑制狀態(tài)。L-929細(xì)胞能在PHB-PEG多聚物中,細(xì)胞毒性評(píng)分均為0分,而在PVC環(huán)境中,細(xì)胞毒性評(píng)分為3分;L-929細(xì)胞能在PHB-PEG多聚物中環(huán)境良好的生長(zhǎng),無(wú)細(xì)胞毒性產(chǎn)生,生物相容性良好,而L-929細(xì)胞在PVC環(huán)境中存活較差。結(jié)論:PHB-PEG多聚物良好的細(xì)胞相容性及其生物性質(zhì),使其可能成為潛在醫(yī)用材料。
[Abstract]:Objective: Poly (尾 -hydroxybutyrate) (Poly 尾 -butyrate) is a kind of aliphatic polyester which can be fermented by a variety of bacteria. It has good biocompatibility, biodegradability and piezoelectric properties. The purpose of this paper is to test the physical properties of poly (尾 -hydroxybutyrate) polymer and to study its biocompatibility with L-929 cells. Methods: in this study, the poly (尾 -hydroxybutyrate) poly (ethylene glycol) (PHB-PEG) copolymers were detected by IR, NMR, DSC, and the physical properties of the copolymers were analyzed. Secondly, L-929 cells were cultured on poly (尾 -hydroxybutyrate) poly (ethylene glycol) polymer film and polyvinyl chloride (PVC) film at high temperature and normal temperature, and the survival of L-929 cells was observed on the second, fourth and sixth day. Results: the results of PHB-PEG copolymer characterization showed that the existence of links between PHB-PEG was confirmed by PHB-PEG IR, and the structure of PHB-PEG copolymers was further proved by Mr spectra. Differential scanning calorimetry (DSC) showed that the transition temperature between different phases of PHB-PEG was lower than that of PHB. The co-existence of L-929 cells and PHB-PEG showed that the cells in PHB-PEG group grew well and denser under high temperature and normal temperature. Some processes began to stretch out, fusiform; In the PV group, cell pyknosis and death began on the second day. There was no significant difference in the growth of L-929 cells between the PHB group and the negative control group (p0.05), but there was significant difference between the PHB group and the positive control group (p0.01) in the growth of L-929 cells in the hyperthermia and normothermic environment. There was significant difference between the positive control PVC group and the negative control NaCl group (p 0.01). The AO values at different time points in the same group were tested by covariance test. The results showed that the growth trend of L-929 cells in NaCl group and PHB-PEG group was the same. In the positive control group, the growth of L-929 cells was inhibited. The cytotoxicity scores of L-929 cells were 0 in PHB-PEG polymers and 3 in PVC. L-929 cells could grow well in the PHB-PEG polymer, had no cytotoxicity and had good biocompatibility, while L-929 cells could not survive in the PVC environment. Conclusion: PHB-PEG polymer has good cytocompatibility and biological properties, which makes it a potential medical material.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R318.08

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊成;孟麗娥;田元;常婷;;新型鈦基溶膠凝膠涂層的細(xì)胞相容性研究[J];生物骨科材料與臨床研究;2006年04期

2 孟德強(qiáng);曾慶敏;熊敏;;氧化鈦涂層及改性后氧化鈦涂層的細(xì)胞相容性研究[J];中國(guó)矯形外科雜志;2011年05期

3 肖榮冬;翁國(guó)星;;血管組織工程支架材料的細(xì)胞相容性[J];中國(guó)組織工程研究;2012年43期

4 張承e，

本文編號(hào)：2429733