【摘要】：人工關(guān)節(jié)的骨溶解(磨屑病)是導(dǎo)致人工假體松動(dòng)的主要原因。利用稀土離子抑制破骨細(xì)胞的活性將會(huì)有效地防治骨溶解的產(chǎn)生。本文利用激光熔覆技術(shù)對(duì)生物醫(yī)用金屬材料(Ti-6Al-4V)表面進(jìn)行改性處理,制備出含有稀土的Ca-P生物陶瓷涂層,利用涂層在生理環(huán)境中析出的稀土離子來達(dá)到防治骨溶解的目的。利用金相顯微鏡(OM)、掃描電鏡(SEM)、等離子質(zhì)譜儀(ICP-AES)、X射線衍射儀(XRD)、顯微硬度儀、MTT比色法、倒置熒光顯微鏡,TRAP染色法等檢測(cè)手段分別對(duì)Ca-P生物陶瓷涂層結(jié)合界面、微觀形貌、稀土離子析出規(guī)律、物相、顯微硬度、生物活性以及稀土離子濃度對(duì)破骨細(xì)胞活性抑制作用的規(guī)律進(jìn)行了研究,并獲得了以下結(jié)論:(1)探究了不同La_2O_3摻雜量及不同鈣磷比對(duì)激光熔覆Ca-P陶瓷涂層組織性能與生物活性的影響。結(jié)果表明:Ca-P陶瓷涂層主要包括CaTiO_3、HA、β-TCP、TiO_2等物相。涂層中Ca:P比值越高,平均硬度越大。La_2O_3摻雜量為0.6 wt.%,Ca:P=1.40的Ca-P陶瓷涂層具有最佳的組織性能與生物活性。(2)探究了Ca-P陶瓷涂層在模擬體液中的降解析出規(guī)律。結(jié)果表明:Ca-P陶瓷涂層在模擬體液中質(zhì)量呈現(xiàn)動(dòng)態(tài)變化的規(guī)律,是類骨磷灰石沉積與β-TCP降解共存的一種動(dòng)態(tài)變化。涂層中的La~(3+)平均每天的析出濃度在9.5~12 PPB之間波動(dòng)變化。并計(jì)算得到Ca-P涂層中La~(3+)析出濃度C與浸泡時(shí)間t之間的規(guī)律公式為:(?),單位(PPB/mm~2)。(3)探究了Ca-P陶瓷涂層中可控析出的稀土離子對(duì)破骨細(xì)胞活性的抑制作用。結(jié)果表明:當(dāng)細(xì)胞誘導(dǎo)因子濃度RANKL為100 ng/mL,M-CSF為50 ng/mL時(shí),誘導(dǎo)生成的陽(yáng)性多核破骨細(xì)胞數(shù)量最多。設(shè)置3天,6天,9天三個(gè)時(shí)間點(diǎn),從統(tǒng)計(jì)數(shù)據(jù)可以看出,與空白組相比,La~(3+)濃度為21 PPB時(shí),誘導(dǎo)生成的破骨細(xì)胞數(shù)量顯著增加,P0.05。其他濃度的La~(3+)對(duì)誘導(dǎo)生成的破骨細(xì)胞數(shù)量具有明顯的抑制作用。綜上可知當(dāng)La_2O_3含量為0.6 wt.%,Ca:P=1.40的Ca-P陶瓷涂層不僅具有優(yōu)異的組織性能及生物活性,并且具有抑制破骨細(xì)胞活性的作用時(shí)間可以長(zhǎng)達(dá)16.5年左右,期望其可為臨床實(shí)踐上防治磨屑病提供一種新思路。
[Abstract]:Osteolysis of artificial joint (debris disease) is the main cause of artificial prosthesis loosening. The inhibition of osteoclast activity by rare earth ions will effectively prevent the formation of osteolysis. In this paper, the surface of biomedical metal material (Ti-6Al-4V) was modified by laser cladding, and the Ca-P bioceramics coating containing rare earth was prepared. The rare earth ions precipitated by the coating in physiological environment were used to prevent and cure osteolysis. Metallographic microscope (OM), scanning electron microscope (SEM), plasma mass spectrometer (ICP-AES), X ray diffractometer (XRD), microhardness instrument MTT colorimetric method inverted fluorescence microscope) was used. The interface of Ca-P bioceramics coating, micromorphology, precipitation regularity of rare earth ions, phase and microhardness of Ca-P bioceramics coating were examined by TRAP staining method. Biological activity and the inhibition of rare earth ion concentration on osteoclast activity were studied. The following conclusions were obtained: (1) the effects of different amounts of La_2O_3 doping and different Ca / P ratios on the microstructure, properties and biological activity of laser clad Ca-P ceramic coatings were investigated. The results show that the Ca-P ceramic coating mainly includes CaTiO_3,HA, 尾-TCP,TiO_2 phase. The higher the Ca:P ratio in the coating, the greater the average hardness. The doping amount of La_2O_3 is 0.6 wt.%,. The Ca-P ceramic coating of Ca:P=1.40 has the best microstructure, properties and biological activity. (2) the degradation and precipitation of Ca-P ceramic coating in simulated body fluid was investigated. The results show that the quality of Ca-P ceramic coating changes dynamically in simulated body fluid, which is a dynamic change of the coexistence of bone-like apatite deposition and 尾-TCP degradation. The average daily precipitation concentration of La~ (3) in the coating fluctuates from 9.5 to 12 PPB. The formula between the concentration C of La~ (3) in Ca-P coating and the soaking time t was calculated as follows: (?) Unit (PPB/mm~2). (3) was used to investigate the inhibitory effect of rare earth ions precipitated in Ca-P ceramic coating on osteoclast activity. The results showed that when the concentration of RANKL was 100 ng/mL,M-CSF and 50 ng/mL, the number of positive multinuclear osteoclasts induced was the highest. When the concentration of La~ (3) was 21 PPB, the number of osteoclasts induced was increased significantly (P0.05). Other concentrations of La~ (3) inhibited the number of osteoclasts induced. It can be concluded that when the content of La_2O_3 is 0.6 wt.%,Ca:P=1.40, the Ca-P ceramic coating not only has excellent microstructure and properties, but also has good bioactivity. The activity of osteoclast can be inhibited for about 16.5 years. It is expected that it can provide a new idea for the prevention and treatment of abrasion disease in clinical practice.
【學(xué)位授予單位】：貴州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R318.08;TG174.4

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