【摘要】：目的用選擇性激光燒結(selective laser sintering,SLS)技術構建納米羥基磷灰石(nano-hydroxyapatite,Nano-HA)/聚己內酯(poly-ε-caprolactone,PCL)人工骨支架并探索其力學特性、生物相容性和生物活性。方法配制NanoHA質量分數(shù)分別為5%、10%、15%的Nano-HA/PCL混合材料粉末,采用SLS技術制備出PCL與Nano-HA/PCL(5%、10%、15%)人工骨支架,并測定其孔隙率及抗壓強度。將分離培養(yǎng)的兔骨髓間充質干細胞(MSCs)接種至上述2種支架中,觀察細胞黏附及增殖情況,并通過堿性磷酸酶(ALP)表達測定、茜素紅法鈣結節(jié)染色比較其成骨分化情況。結果各組支架均具有良好的力學強度和細胞黏附性,且未見明顯的細胞毒性;接種后第1天各組的ALP表達差異無統(tǒng)計學意義,但隨著培養(yǎng)時間的延長,Nano-HA/PCL組的ALP表達逐漸高于PCL組與空白對照組(P0.05),且Nano-HA比例越高,ALP的表達也越強(P0.05);Nano-HA/PCL組茜素紅染色的陽性強度高于PCL組與空白對照組,且隨著Nano-HA含量增高,其鈣結節(jié)數(shù)量增多,染色的陽性強度也隨之增加。結論本研究通過SLS技術所構建出的Nano-HA/PCL人工骨支架具有良好的生物力學強度、生物相容性及骨誘導性,有望成為一種新型骨修復替代材料。
[Abstract]:Objective to construct nano-hydroxyapatite (nano-hydroxyapatite,Nano-HA) / polycaprolactone (poly- 蔚-caprolactone,PCL) artificial bone scaffold by selective laser sintering (selective laser sintering,SLS) and explore its mechanical properties, biocompatibility and bioactivity. Methods the PCL and Nano-HA/PCL artificial bone scaffolds were prepared by SLS technique, and the porosity and compressive strength of the scaffolds were measured. Cultured rabbit bone marrow mesenchymal stem cells (MSCs) were inoculated into the above two scaffolds to observe the cell adhesion and proliferation. The osteogenic differentiation of rabbit bone marrow mesenchymal stem cells was determined by alkaline phosphatase (ALP) expression and the osteogenic differentiation was compared by alizarin red calcium nodule staining. Results the scaffolds had good mechanical strength and cell adhesion, and there was no obvious cytotoxicity. On the first day after inoculation, there was no significant difference in ALP expression in all groups, but with the extension of culture time, the ALP expression in Nano-HA/PCL group was gradually higher than that in PCL group and blank control group (P0.05), and the ratio of Nano-HA was higher than that in control group. The expression of ALP was stronger (P0.05). The positive intensity of alizarin red staining in Nano-HA/PCL group was higher than that in PCL group and blank control group. With the increase of Nano-HA content, the number of calcium nodules increased and the positive intensity of staining increased. Conclusion the Nano-HA/PCL artificial bone scaffold constructed by SLS technique has good biomechanical strength, biocompatibility and bone induction, and it is expected to be a new substitute for bone repair.
【作者單位】： 第二軍醫(yī)大學長海醫(yī)院創(chuàng)傷骨科;解放軍309醫(yī)院骨科;解放軍94826部隊野戰(zhàn)醫(yī)院;蘭州軍區(qū)總醫(yī)院骨科;解放軍71677部隊衛(wèi)生隊;
【基金】：國家杰出青年科學基金(81201442)~~
【分類號】：R318.08

【相似文獻】

相關期刊論文 前6條

1 王月波;汪焰恩;魏生民;楊明明;;人工骨支架結構設計與實現(xiàn)[J];西安工業(yè)大學學報;2012年04期

2 魏慶華;汪焰恩;楊明明;魏生民;;基于離散單元法多孔陶瓷微球骨支架的微觀結構分析[J];工具技術;2013年12期

3 胡發(fā)宗,趙毅,陳罡,閆煥營;三維立體打印機成形人工骨支架[J];現(xiàn)代制造工程;2004年10期

4 戴伯軍,李家順,賈連順,呂宏,陳崗,葉曉健,蔡舒,張立德;成骨細胞株與強韌化納米人工骨支架聯(lián)合培養(yǎng)實驗研究[J];中國矯形外科雜志;2004年12期

5 陳崗,李家順,賈連順,呂宏,葉曉健,戴伯軍,蔡舒,張立德;成骨細胞(Hos8603)與強韌化納米人工骨支架的聯(lián)合培養(yǎng)[J];第二軍醫(yī)大學學報;2005年01期

6 ;[J];;年期

相關博士學位論文 前1條

1 林山;數(shù)字化珊瑚羥基磷灰石人工骨支架的制備及生物學評價[D];南方醫(yī)科大學;2011年

相關碩士學位論文 前3條

1 王振華;基于熔融沉積法的人工骨支架快速成型機噴絲技術研究[D];哈爾濱工業(yè)大學;2014年

2 莊靜宇;選擇性激光燒結制備磷酸三鈣人工骨支架的研究[D];中南大學;2013年

3 禹娜娜;人工骨支架內孔系結構設計及內部流場的CFD分析[D];燕山大學;2011年

，

本文編號：2416171