【摘要】：跟腱疾病通常是由于過度勞損造成的,常發(fā)生于體育運(yùn)動(dòng)、繁重體力勞動(dòng)等場(chǎng)合,呈逐年遞增趨勢(shì)。肌腱損傷常常不能自愈,治療這一運(yùn)動(dòng)疾病需要生物工程學(xué)家的參與。本研究設(shè)計(jì)了一種新的、精確易用的冷凍夾具,解決了以往肌腱損傷修復(fù)基礎(chǔ)研究中力學(xué)測(cè)試?yán)щy的困境。該冷凍夾具通過液氮將預(yù)冷的肌肉在力學(xué)測(cè)試過程中保持在冷凍狀態(tài),從而解決了拉伸測(cè)試過程中肌肉/肌腱的相對(duì)滑移和應(yīng)力集中問題。該夾具還設(shè)計(jì)了一個(gè)生理鹽水浴環(huán)境,從而使整個(gè)測(cè)試過程很好的模擬了生理環(huán)境,保證了體外測(cè)試數(shù)據(jù)能真實(shí)體現(xiàn)體內(nèi)生理狀況。 本研究以大鼠跟腱為對(duì)象,利用設(shè)計(jì)的冷凍夾具進(jìn)行拉伸測(cè)試。共20個(gè)待測(cè)試樣,隨機(jī)分為4組,分別測(cè)試不同預(yù)冷方法、樣品拉伸環(huán)境、夾具移動(dòng)速度下跟腱的力學(xué)特性。具體分組和測(cè)試方法如下：A組,將樣品放置-20℃冷凍1周,生理鹽水浴環(huán)境拉伸,夾具移動(dòng)速度為10mm/min;B組,將樣品放置液氮速凍1分鐘,生理鹽水浴環(huán)境拉伸,夾具移動(dòng)速度10mm/min;C組,將樣品放置液氮速凍1分鐘,室溫(25℃)空氣環(huán)境拉伸,夾具移動(dòng)速度10mm/min;D組,將樣品放置液氮速凍1分鐘,室溫(25℃)空氣環(huán)境拉伸,夾具移動(dòng)速度500mm/min。記錄力-位移曲線,本研究獲取了4組樣品的最大斷裂力、斷裂點(diǎn)最大位移、楊氏模量,結(jié)果分別如下：A組,119.2±25N,1.868±0.174mm,264.64±108.78MPa;B組,90.5±13N,3.6838±0.8825mm,66.926±24.743MPa;C組,70±20N,2.966±1.279mm,86.822±27.915MPa;D組,53±10N,2.130±0.719mm,38.078±9.765MPa。結(jié)果經(jīng)過統(tǒng)計(jì)分析表明：-20℃冷凍1周和液氮速凍1分鐘的樣品處理方式最大斷裂力無顯著差異,生理鹽水浴的拉伸環(huán)境最大斷裂力顯著大于室溫(25℃)空氣環(huán)境,夾具移動(dòng)速度在低速(10mm/min)和高速(500mm/min)時(shí)最大斷裂力無顯著差異,但低速時(shí)斷裂點(diǎn)最大位移更均一。
[Abstract]:Achilles tendon disease is usually caused by overstrain, often occurs in sports, heavy physical labor and other occasions, showing an increasing trend year by year. Tendon injuries often fail to heal, and the treatment of the disease requires the involvement of bioengineers. In this study, a new, accurate and easy to use freezing fixture was designed to solve the difficulty of mechanical testing in previous basic researches of tendon repair. The freezing fixture keeps the precooled muscles frozen in the mechanical testing process by liquid nitrogen, thus solving the problems of relative slip and stress concentration of the muscle / tendon during the tensile testing. The fixture also designed a physiological saline bath environment, so that the whole testing process is very good simulation of the physiological environment, to ensure that in vitro test data can truly reflect the physiological situation in vivo. In this study, the Achilles tendon of rats was used for tensile test with the designed freezing fixture. A total of 20 samples were randomly divided into 4 groups. The mechanical properties of Achilles tendon under different precooling methods, tensile environment and moving speed of clamp were tested respectively. The specific grouping and testing methods were as follows: group A, the samples were frozen at -20 鈩，

本文編號(hào)：2406912