發(fā)布時(shí)間：2019-01-07 22:12

【摘要】：目的開(kāi)發(fā)生產(chǎn)人纖維蛋白粘合劑產(chǎn)品,并對(duì)其成膠速度、持續(xù)時(shí)間、彈力及抗張強(qiáng)度等凝膠性能指標(biāo)進(jìn)行研究。方法通過(guò)乙醇沉淀,離子交換層析等分離純化方法和多重病毒滅活工藝制備產(chǎn)品;體外拉伸試驗(yàn)和小鼠試驗(yàn)研究其凝膠性能指標(biāo)。結(jié)果所制得纖維蛋白原及凝血酶各項(xiàng)性能指標(biāo)均優(yōu)于中國(guó)藥典標(biāo)準(zhǔn),纖維蛋白粘合劑成品成膠時(shí)間均在2 s以內(nèi),具有較快的成膠速度;37℃下凝膠持續(xù)時(shí)間在600-700 min內(nèi),使用牛抑肽酶試液溶解纖維蛋白原的試驗(yàn)組成膠速度有所降低,而凝膠的持續(xù)時(shí)間有所延長(zhǎng);纖維蛋白粘合劑平均單位面積拉伸高度6.4-7.4 mm,具有較好的彈性指數(shù),而抗張強(qiáng)度差異較大,最佳抗張強(qiáng)度為80 g/cm2,抗張強(qiáng)度方面有所不足。結(jié)論所開(kāi)發(fā)的人纖維蛋白粘合劑制品具有高強(qiáng)度、高彈力、成膠速度快,持續(xù)時(shí)間久的凝膠性能。
[Abstract]:Objective to develop and produce human fibrin binder and to study the gel properties such as gelation speed, duration, elasticity and tensile strength. Methods the product was prepared by ethanol precipitation, ion exchange chromatography and multiplex virus inactivation, and its gel properties were studied by tensile test and mouse test in vitro. Results the properties of fibrinogen and thrombin were superior to those of Chinese pharmacopoeia standard. The gelation time of fibrin binder was less than 2 seconds, and the gelation rate of fibrinogen binder was faster than that of Chinese pharmacopoeia. When the gel duration was between 600-700 min at 37 鈩，

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