發(fā)布時間：2018-10-23 18:07

【摘要】：傳統(tǒng)的水凝膠支架面臨傷口創(chuàng)傷、感染以及對缺損部位不適應(yīng)等問題,從而導(dǎo)致支架的失效�？勺⑸錅孛羲z在注射前為流動的液體,通過注射器注入皮下組織或肌肉組織后,可在注射部位原位凝膠化,具有微創(chuàng)易治療、可修復(fù)深層組織損傷、不破壞修復(fù)區(qū)供血等優(yōu)點(diǎn)。通常血管內(nèi)皮生長因子(VEGF)和基質(zhì)細(xì)胞衍生因子-1(SDF-1)的半衰期較短,若將其裝載到納米顆粒復(fù)合凝膠中則可起緩釋效果。因此實(shí)驗(yàn)制備了殼聚糖基水凝膠,保持殼聚糖和明膠體積比不變,通過加入不同體積的β-甘油磷酸鈉,制備出不同組分的殼聚糖基溫敏水凝膠體系,研究了不同凝膠的成膠時間、孔隙率以及細(xì)胞毒性;并制備了殼聚糖和巖藻聚糖的納米顆粒,對VEGF和SDF-1進(jìn)行了裝載,并對納米顆粒的粒徑、電位及因子釋放做了檢測。結(jié)果表明殼聚糖基水凝膠具有溫敏性且增加β-甘油磷酸鈉的量會降低凝膠時間,凝膠電鏡觀察呈三維網(wǎng)絡(luò)狀,無細(xì)胞毒性;納米顆粒粒徑分布較均勻且穩(wěn)定,在凝膠中有緩釋效果。因此,制備安全可靠、有合適響應(yīng)速度的殼聚糖基納米復(fù)合水凝膠是提高材料性能的一種有效方式,將為今后滿足不同需求的醫(yī)用生物材料提供新的研究方向。
[Abstract]:Traditional hydrogel scaffolds face wound trauma, infection and failure to adapt to the defect, which leads to the failure of the scaffold. Injectable thermo-sensitive hydrogel is a fluid flowing before injection. After injecting subcutaneous tissue or muscle tissue through syringe, it can be gelled in situ at the injection site, which is minimally invasive and easy to treat, and can repair deep tissue damage. Do not destroy the repair area of blood supply and other advantages. The half-life of vascular endothelial growth factor (VEGF) and stromal cell derived factor-1 (SDF-1) is usually shorter, but it can be released slowly if loaded into nano-particle composite gel. Therefore, chitosan based hydrogels were prepared, which kept the volume ratio of chitosan to gelatin unchanged. By adding different volumes of 尾 -glycerophosphate, different components of chitosan based thermo-sensitive hydrogels were prepared. The gelation time, porosity and cytotoxicity of different gels were studied, and the nanoparticles of chitosan and fucosan were prepared, VEGF and SDF-1 were loaded, and the particle size, potential and factor release were determined. The results showed that chitosan hydrogel had temperature sensitivity and increased the amount of 尾 -glycerophosphate, and the gel time was decreased. The gel electron microscope showed three-dimensional network shape, no cytotoxicity, and the particle size distribution was uniform and stable. It has slow release effect in gel. Therefore, the preparation of chitosan based nanocomposite hydrogels, which is safe and reliable and has a suitable response speed, is an effective way to improve the properties of the materials. It will provide a new research direction for medical biomaterials that meet different requirements in the future.
【作者單位】： 西南交通大學(xué)生命科學(xué)與工程學(xué)院;西南交通大學(xué)材料先進(jìn)技術(shù)教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(NSFC81401522) 中央高�；究蒲袠I(yè)務(wù)費(fèi)專項(xiàng)資金,醫(yī)學(xué)類專題研究資助項(xiàng)目(2682016YXZT15)
【分類號】：R318.08

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