【摘要】：細(xì)胞的彈性性質(zhì)是細(xì)胞力學(xué)的一個重要參數(shù)，細(xì)胞的結(jié)構(gòu)和功能與細(xì)胞的力學(xué)性質(zhì)密切相關(guān)。外界物理環(huán)境的改變，如細(xì)胞外基質(zhì)軟硬度的變化會導(dǎo)致細(xì)胞彈性發(fā)生變化。原子力顯微鏡可以在生理條件下檢測樣品表面的力學(xué)性質(zhì)，是研究細(xì)胞力學(xué)的一個十分有用的工具。 本文以殼聚糖為材料制備了不同軟硬的基底膜，利用原子力顯微鏡研究殼聚糖膜對細(xì)胞彈性的影響。通過配制不同濃度的殼聚糖溶液，采用流延法制備了實(shí)驗所需的殼聚糖膜，并對殼聚糖膜的細(xì)胞相容性、接觸角、硬度做了測定。將正常的內(nèi)皮細(xì)胞和肝癌細(xì)胞分別接種在不同濃度的殼聚糖膜上，對其形態(tài)特征（細(xì)胞鋪展面積、細(xì)胞長、短軸比值等）和細(xì)胞彈性做了檢測。本文的主要結(jié)果如下：①殼聚糖膜溶脹性最大的為3%殼聚糖膜，最小的為4%殼聚糖膜；膜的接觸角約在65°～81°之間，膜的硬度（復(fù)水后）約在315KPa～413KPa之間，膜的接觸角和硬度隨著制膜溶液濃度的增大而增大；②內(nèi)皮細(xì)胞和肝癌細(xì)胞在膜上鋪展的面積隨著制膜時溶液濃度的增大而增大，細(xì)胞長軸與短軸的比率在不同濃度的殼聚糖膜上有所不同，，說明細(xì)胞形態(tài)在不同的殼聚糖膜上會發(fā)生變化；內(nèi)皮細(xì)胞最大的彈性模量約為2.44KPa，最小的約為1.75KPa,最大的彈性模量值是最小模量值的1.4倍，肝癌細(xì)胞最大的彈性模量約為1.32KPa，最小的約為0.66KPa，最大的彈性模量值是最小模量值的2倍。這說明不同的殼聚糖膜對細(xì)胞的彈性模量有著明顯地影響。實(shí)驗結(jié)果有助于了解殼聚糖膜與細(xì)胞之間的相互作用，為殼聚糖膜在細(xì)胞生物學(xué)領(lǐng)域的研究和應(yīng)用增加理論上的認(rèn)識。
[Abstract]:The elastic property of cells is an important parameter of cellular mechanics. The structure and function of cells are closely related to the mechanical properties of cells. Changes in physical environment, such as the degree of hardness of extracellular matrix, will lead to changes in cell elasticity. Atomic force microscope (AFM) is a useful tool for studying cellular mechanics because it can detect the mechanical properties of samples under physiological conditions. In this paper, different soft and hard substrate membranes were prepared from chitosan, and the effect of chitosan membrane on cell elasticity was studied by atomic force microscopy (AFM). The chitosan membrane was prepared by different concentration of chitosan solution, and the cell compatibility, contact angle and hardness of chitosan membrane were measured. Normal endothelial cells and hepatoma cells were inoculated on chitosan membrane at different concentrations, and their morphological characteristics (cell spreading area, cell length, short axis ratio, etc.) and cell elasticity were determined. The main results are as follows: (1) the highest swelling property of chitosan membrane is 3% chitosan film, the smallest is 4% chitosan film, the contact angle of the membrane is about 65 擄~ 81 擄, the hardness of the membrane is about 315KPa~413KPa. The contact angle and hardness of the membrane increased with the increase of the concentration of the solution, 2 the area of the endothelial cells and hepatoma cells spreading on the membrane increased with the increase of the concentration of the solution. The ratio of cell long axis to short axis was different in different concentrations of chitosan membrane, which indicated that cell morphology would change on different chitosan membrane. The maximum elastic modulus of endothelial cells is about 2.44 KPA, the smallest is about 1.75 KPA, the maximum elastic modulus is 1.4 times of the minimum, the maximum elastic modulus of hepatoma cells is about 1.32 KPA, the smallest is about 0.66 KPA, and the maximum elastic modulus is 2 times of the minimum. This shows that different chitosan membranes have a significant effect on the elastic modulus of cells. The experimental results are helpful to understand the interaction between chitosan membrane and cells, and to increase the theoretical understanding of the research and application of chitosan membrane in the field of cell biology.
【學(xué)位授予單位】：重慶大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.01

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