【摘要】：在乙醇介質(zhì)中使用乙二醇縮水甘油醚(EGDE)交聯(lián)脫細胞豬真皮基質(zhì)(pADM),優(yōu)化了最佳交聯(lián)條件;考察了交聯(lián)pADM(EGDE-pADM)的改性指數(shù)、傅里葉紅外光譜、機械性能、親水性、掃描電鏡及細胞相容性;通過對pADM與皮粉進行封閉氨基及封閉羧基來初步探索EGDE與pADM的相互作用。研究結(jié)果表明,交聯(lián)得到的EGDE-pADM的收縮溫度隨用量、溫度、時間的增加而先增大后逐漸趨于穩(wěn)定;交聯(lián)最優(yōu)條件為溫度37℃、用量16%、反應時間48h。在最優(yōu)條件下得到的交聯(lián)材料物理機械性能與親水性得到提高,細胞毒性為Ⅰ級,形態(tài)較優(yōu),材料表面有利于細胞粘附生長。相互作用的研究表明,乙醇介質(zhì)中EGDE主要與pADM上的氨基發(fā)生交聯(lián)反應,也能與其它活性基團發(fā)生反應。
[Abstract]:The optimum crosslinking conditions were optimized by using glycidyl ether (EGDE) crosslinked acellular porcine dermal matrix (pADM), in ethanol medium, the modification index, Fourier transform infrared spectroscopy, mechanical properties and hydrophilicity of cross-linked pADM (EGDE-pADM) were investigated. Scanning electron microscopy (SEM) and cytocompatibility were used to study the interaction between EGDE and pADM by blocking amino and carboxyl groups between pADM and skin powder. The results show that the shrinkage temperature of the crosslinked EGDE-pADM increases first and then becomes stable with the increase of the amount, temperature and time of crosslinking, and the optimum crosslinking conditions are 37 鈩，

本文編號：2269603