【摘要】：目的通過成骨細胞的體外培養(yǎng),初步探討鈦及鈦合金微-納米三維形貌對成骨細胞生物學行為的影響。方法采用電解蝕刻法在純鈦及鈦合金表面構(gòu)建出不同尺寸的微-納米三維形貌,并觀察其三維結(jié)構(gòu)表面對成骨細胞黏附、增殖、細胞形態(tài)、堿性磷酸酶(ALP)活性的影響。結(jié)果在成骨細胞的黏附和增殖方面,純鈦組和鈦合金組表面均高于純鈦機械拋光組。純鈦組表面細胞胞體飽滿,伸出大量偽足,并可見大量功能顆粒。ALP活性顯著高于鈦合金和純鈦機械拋光組表面。結(jié)論通過電解蝕刻法在純鈦和鈦合金表面可形成不同直徑和深度的碗形巢樣及納米結(jié)構(gòu);兩個表面即30~50μm和5~8μm的表面和光滑表面相比,都明顯促進了細胞的附著;30~50μm的純鈦表面更有利于促進細胞的增殖和分化。
[Abstract]:Objective to investigate the effect of titanium and titanium alloy micromorphology on the biological behavior of osteoblasts in vitro. Methods three dimensional microstructures were constructed on the surface of pure titanium and titanium alloy by electrolytic etching, and the effects of three dimensional structure on the adhesion, proliferation, cell morphology and alkaline phosphatase (ALP) activity of osteoblasts were observed. Results in the aspect of osteoblast adhesion and proliferation, the surface of pure titanium group and titanium alloy group were higher than that of pure titanium mechanical polishing group. In pure titanium group, the cell body was full, a large number of pseudopodia were extended, and a large number of functional particles could be seen. The activity of ALP was significantly higher than that of titanium alloy and pure titanium mechanically polished group. Conclusion different diameters and depths of cut-shaped nests and nanostructures can be formed on pure titanium and titanium alloy surfaces by electrolytic etching, and the two surfaces, 30 ~ 50 渭 m and 5 ~ 8 渭 m, are compared with smooth surfaces. The surface of pure titanium at 30 渭 m was more favorable to the proliferation and differentiation of the cells.
【作者單位】： 德州市人民醫(yī)院口腔科;吉林大學口腔醫(yī)院口腔種植中心;濱州醫(yī)學院附屬醫(yī)院口腔科;
【基金】：吉林省科技廳基金資助項目(200905175)
【分類號】：R783.1

【共引文獻】

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2 王敬旭;激光酸蝕聯(lián)合納米管與噴砂酸蝕的鈦種植體表面的對比性研究[D];南方醫(yī)科大學;2014年

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