【摘要】：背景:研究發(fā)現(xiàn)妊娠與干細胞的活性有明顯關(guān)聯(lián)。然而,尚未見到有關(guān)妊娠對脂肪干細胞影響的相關(guān)報道。目的:評估妊娠狀態(tài)對大鼠脂肪干細胞增殖能力和誘導分化為類肝細胞潛能的影響。方法:分別獲取正常大鼠和妊娠大鼠2種來源的脂肪干細胞進行原代分離、培養(yǎng)和鑒定。利用CCK8法檢測脂肪干細胞的增殖能力。采用相同肝誘導培養(yǎng)基,誘導脂肪干細胞分化為類肝細胞。熒光定量PCR檢測誘導第0,7,14,21天時肝細胞標志物的表達;ELISA方法檢測白蛋白分泌和尿素合成水平。結(jié)果與結(jié)論:(1)成功分離培養(yǎng)出正常大鼠和妊娠大鼠來源脂肪干細胞,經(jīng)流式細胞術(shù)鑒定符合干細胞一般特性(CD44+/CD45-/CD90+);(2)與正常大鼠來源脂肪干細胞相比較,妊娠大鼠來源脂肪干細胞增殖更快,誘導分化為成骨、成脂和成肝細胞時間更短,肝細胞標志物變化更加迅速,白蛋白分泌和尿素合成能力更高;(3)妊娠對脂肪干細胞的增殖和分化具有促進作用,推測可能與雌激素水平相關(guān)。
[Abstract]:Background: pregnancy has been found to be associated with stem cell activity. However, there are no reports of the effects of pregnancy on adipose stem cells. Aim: to evaluate the effects of pregnancy on the proliferation and differentiation of adipose stem cells in rats. Methods: two kinds of adipose stem cells from normal and pregnant rats were isolated, cultured and identified. The proliferative ability of adipose stem cells was detected by CCK8 method. Adipose stem cells were induced to differentiate into hepatocyte-like cells on the same liver induction medium. Fluorescence quantitative PCR was used to detect the expression of hepatocyte markers and the levels of albumin secretion and urea synthesis were detected by ELISA. Results and conclusion: (1) adipose stem cells derived from normal and pregnant rats were isolated and cultured successfully. The results of flow cytometry showed that adipose stem cells accorded with the general characteristics of stem cells (CD44 / CD45-/CD90); (2) and were compared with those derived from normal rats. Adipose stem cells derived from pregnant rats proliferate more rapidly, induce differentiation into osteoblasts, shorten the time of adipogenesis and hepatoblast, and change the markers of liver cells more rapidly. (3) pregnancy can promote the proliferation and differentiation of adipose stem cells, which may be related to estrogen level.
【作者單位】： 昆明醫(yī)科大學第四附屬醫(yī)院骨與創(chuàng)傷外科;昆明醫(yī)科大學第二附屬醫(yī)院急診醫(yī)學部;
【分類號】：R318;R575

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