【摘要】：鈦及鈦合金以其優(yōu)良的物理化學性能(如較強的耐腐蝕性,較高的韌性和熔點及質(zhì)量較輕等)在種植牙領域得到廣泛應用,但可能由于種植體周圍發(fā)生感染以及缺乏良好的骨-種植體整合而導致種植體植入失敗。研究表明,通過對種植體表面改性可提高種植牙的成功率。近年來,研究的重點主要集中在以下一些表面改性方法：通過改善表面可濕性、設計表面粗糙度及形貌、制備與骨成分相近的涂層等方法提高種植體與周圍骨組織整合；通過種植體表面修飾抗菌劑或制備功能性涂層作為抗菌元素的載體等方式降低種植體周圍感染的發(fā)生率。但是,既能促進骨細胞增殖同時降低種植體周圍感染發(fā)生的簡單、有效的表面改性方法目前還未見報道。 在本研究中,我們利用飛秒激光蒸鍍摻銀改性技術對種植體表面進行改性,期望促進種植體周圍骨細胞增殖的同時降低種植體周圍感染的發(fā)生率。飛秒激光以其高精確度、表面形貌可控性、無污染等特性在材料(金屬、半導體以及陶瓷材料等)表面改性領域得到廣泛應用,能夠有效改變材料的光學、電學、力學及磁學性能。在本研究中,將飛秒激光應用于對鈦及鈦合金的表面改性中,研究飛秒激光誘導材料表面形成不同的周期性結構及其對細胞增殖和抗菌性的影響。通過改變飛秒激光能量參數(shù),鈦表面形成不同尺度的周期性結構：當激光的能量密度為0.07J/cm2時,鈦表面形成周期性納米級條紋結構；當激光的能量密度為1.4J/cm2時,鈦表面形成微米級凸起與納米級條紋疊加的復合結構,將其與細胞共培養(yǎng),我們發(fā)現(xiàn)微納米復合結構表面有利于成骨細胞的黏附和增殖。我們又將飛秒激光技術與蒸鍍摻銀技術結合,在制備微納米復合結構的同時實現(xiàn)對種植體表面的可控性銀摻雜,提高了種植體的生物相容性及抗菌性。細胞毒性實驗結果顯示,在銀的摻雜量較小時,種植體沒有細胞毒性,并有一定的促進細胞增殖的作用�？咕缘难芯拷Y果表明,隨著銀含量的增加,種植體的抗菌能力逐漸增加。 綜上所述,我們利用飛秒激光蒸鍍摻銀改性技術對鈦種植體表面進行形貌改變及銀的可控性摻雜,并進行體外實驗,結果證實飛秒激光加工形成的微納米復合結構提高了鈦的生物相容性；摻雜適當?shù)你y可以促進細胞的黏附和增殖,提高了鈦的生物相容性,鈦的抗菌性隨著銀含量的增加逐漸增強。以上結果表明飛秒激光蒸鍍摻銀技術可達到促進成骨細胞的增殖,提高種植材料的生物相容性,同時降低細菌感染的雙重作用,為鈦種植體的體內(nèi)實驗及臨床應用奠定了基礎。
[Abstract]:Titanium and titanium alloys are widely used in the field of dental implants for their excellent physical and chemical properties, such as strong corrosion resistance, higher toughness, lower melting point and lower mass. However, implants may fail due to infection around implants and lack of good osseous-implant integration. The results showed that the success rate of implant teeth could be improved by surface modification of implants. In recent years, the research focuses on the following methods of surface modification: improving surface wettability, designing surface roughness and morphology, preparing coatings similar to bone composition, improving the integration of implants and surrounding bone tissues; The incidence of peri-implant infection was reduced by surface modification of antibacterial agent or preparation of functional coating as carrier of antimicrobial elements. However, a simple and effective surface modification method has not been reported, which can not only promote the proliferation of bone cells but also reduce the incidence of peri-implant infection. In this study, we modified the implant surface by femtosecond laser evaporation with silver doping, in order to promote the proliferation of bone cells around the implant and reduce the incidence of infection around the implant. Femtosecond laser has been widely used in the field of surface modification of materials (metal, semiconductor, ceramic, etc.) because of its high accuracy, controllable surface morphology and no pollution, which can effectively change the optical and electrical properties of materials. Mechanical and magnetic properties. In this study, femtosecond laser was applied to the surface modification of titanium and titanium alloys. The effects of femtosecond laser on the formation of different periodic structures on the surface of titanium and titanium alloys and their effects on cell proliferation and antibacterial activity were studied. By changing the energy parameters of femtosecond laser, different scale periodic structures are formed on the titanium surface: when the laser energy density is 0.07J/cm2, the titanium surface forms periodic nanoscale stripe structure, and when the laser energy density is 1.4J/cm2, The composite structure of micrometer protuberance and nanometer stripe was formed on the surface of titanium and co-cultured with cells. We found that the surface of micro-nano composite structure is conducive to the adhesion and proliferation of osteoblasts. We also combine femtosecond laser technology with vaporizing silver-doped technology to fabricate micro-nano composite structure and achieve controllable silver doping on implant surface, which improves the biocompatibility and antibacterial property of implant. The results of cytotoxicity test showed that the implants had no cytotoxicity and promoted the proliferation of cells in a small amount of silver doping. The results showed that the antibacterial ability of the implants increased with the increase of silver content. To sum up, we use femtosecond laser vaporizing silver-doped technology to modify the surface morphology of titanium implants and the controllable doping of silver, and conduct experiments in vitro. The results show that the microcomposite structure formed by femtosecond laser processing can improve the biocompatibility of titanium, and the addition of appropriate silver can promote cell adhesion and proliferation, and improve the biocompatibility of titanium. The antibacterial activity of titanium increases with the increase of silver content. The results show that femtosecond laser evaporation and silver doping can promote the proliferation of osteoblasts, improve the biocompatibility of implant materials, and reduce the double effect of bacterial infection, which lays a foundation for the in vivo experiment and clinical application of titanium implants.
【學位授予單位】：吉林大學
【學位級別】：博士
【學位授予年份】：2013
【分類號】：R783.1

【參考文獻】

相關期刊論文 前4條

1 ;Preparation of porous microstructures on NiTi alloy surface with femtosecond laser pulses[J];Chinese Science Bulletin;2008年05期

2 賀剛;陳治清;盛祖立;;純鈦種植體表面納米含氟磷灰石涂層的構建和表征[J];中國口腔種植學雜志;2007年02期

3 賈爽;范震;蘇劍生;;種植體表面粗糙度對骨結合影響的研究現(xiàn)狀[J];中國口腔種植學雜志;2007年02期

4 王玨;焦艷軍;;種植體周圍炎的微生物學研究[J];中國口腔種植學雜志;2008年01期

，

本文編號：2231951