MgZnMn和MgZnRe合金的體外生物相容性評價
發(fā)布時間：2018-08-30 16:43
【摘要】：生物可降解支架的研究已經(jīng)成為當今心血管介入治療的重點發(fā)展方向。支架的如期降解可以克服支架自身的血栓源性及異物性。而可降解支架中,由于鎂和鎂合金的生物可降解性和良好的生物相容性,使得鎂和鎂合金可降解支架受到材料學和醫(yī)學界的廣泛關(guān)注。然而,由于鎂和鎂合金的化學性質(zhì)活潑,耐蝕性較差,阻礙了鎂和鎂合金可降解支架的研究發(fā)展。 本文主要是對課題組自行熔煉制備的鎂鋅錳(MgZnMn)合金和鎂鋅稀土(MgZnRe)合金進行初步的生物相容性研究,為后期的MgZnMn合金和MgZnRe合金的性能改進、表面改性等提供參考。 本文通過析氫腐蝕實驗評價了MgZnMn、MgZnRe以及商用AZ31B鎂合金的耐腐蝕性能。采用體外血小板粘附,凝血時間以及溶血率測試評價了三種合金的血液相容性。利用細胞凋亡染色、細胞增殖實驗和細胞計數(shù)等方法評價了三種鎂合金的細胞相容性。 由實驗數(shù)據(jù)得到以下結(jié)論：析氫腐蝕實驗結(jié)果表明,MgZnMn合金和MgZnRe合金的耐腐蝕性能基本相當,且都明顯優(yōu)于商用的AZ31B鎂合金。血液相容性結(jié)果顯示,與AZ31B合金和316L SS樣品相比較,MgZnMn合金和MgZnRe合金表面粘附血小板數(shù)量較少且基本無團聚現(xiàn)象。而MgZnMn合金和MgZnRe合金的凝血時間與血漿相比也有明顯的延長,同時MgZnMn和MgZnRe的溶血率分別為4.7%、4.4%符合相關(guān)的國家標準,且顯著低于AZ31B合金的80.9%,因此MgZnMn和MgZnRe合金具有較好的血液相容性。細胞相容性評價的細胞凋亡染色(A0/PI染色)、細胞增殖評價(CCK-8)、細胞計數(shù)結(jié)果都一致表明：MgZnMn合金和MgZnRe合金的細胞相容性明顯優(yōu)于商用AZ31B合金。
[Abstract]:Biodegradable stents have become the focus of cardiovascular interventional therapy. Stent degradation on time can overcome the thrombogenic and foreign body properties of stent itself. Because of the biodegradability and good biocompatibility of magnesium and magnesium alloys, the biodegradable scaffolds of magnesium and magnesium alloys have attracted wide attention in the field of materials and medicine. However, the chemical activity and corrosion resistance of magnesium and magnesium alloys hinder the research and development of magnesium and magnesium alloy degradable scaffolds. In this paper, the biocompatibility of mg, Zn, mn, (MgZnMn) and Mg-Zn rare earth (MgZnRe) alloys prepared by our own melting is studied, which provides a reference for the improvement of properties and surface modification of MgZnMn and MgZnRe alloys. The corrosion resistance of MgZnMn,MgZnRe and commercial AZ31B magnesium alloy was evaluated by hydrogen evolution corrosion test. In vitro platelet adhesion, coagulation time and hemolysis rate were used to evaluate the blood compatibility of the three alloys. The cytocompatibility of three magnesium alloys was evaluated by cell apoptosis staining, cell proliferation test and cell count. The results of hydrogen evolution corrosion test show that the corrosion resistance of mg Zn mn alloy and MgZnRe alloy are basically the same, and both of them are obviously superior to commercial AZ31B magnesium alloy. The results of blood compatibility showed that compared with the samples of AZ31B and 316L SS, the number of platelet adhesion on the surface of Mg-ZnMn alloy and MgZnRe alloy was less and there was almost no aggregation. The coagulation time of MgZnMn alloy and MgZnRe alloy was significantly longer than that of plasma, and the hemolysis rate of MgZnMn and MgZnRe were 4.7% and 4.4% respectively, which were lower than that of AZ31B alloy 80.9%. Therefore, MgZnMn and MgZnRe alloy had better blood compatibility. The results of apoptosis staining (A0/PI staining), cell proliferation evaluation (CCK-8) and cell counting showed that the cytocompatibility of MgZnRe alloy and MgZnRe alloy was significantly better than that of commercial AZ31B alloy.
【學位授予單位】：西南交通大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R318.08
本文編號：2213665
[Abstract]:Biodegradable stents have become the focus of cardiovascular interventional therapy. Stent degradation on time can overcome the thrombogenic and foreign body properties of stent itself. Because of the biodegradability and good biocompatibility of magnesium and magnesium alloys, the biodegradable scaffolds of magnesium and magnesium alloys have attracted wide attention in the field of materials and medicine. However, the chemical activity and corrosion resistance of magnesium and magnesium alloys hinder the research and development of magnesium and magnesium alloy degradable scaffolds. In this paper, the biocompatibility of mg, Zn, mn, (MgZnMn) and Mg-Zn rare earth (MgZnRe) alloys prepared by our own melting is studied, which provides a reference for the improvement of properties and surface modification of MgZnMn and MgZnRe alloys. The corrosion resistance of MgZnMn,MgZnRe and commercial AZ31B magnesium alloy was evaluated by hydrogen evolution corrosion test. In vitro platelet adhesion, coagulation time and hemolysis rate were used to evaluate the blood compatibility of the three alloys. The cytocompatibility of three magnesium alloys was evaluated by cell apoptosis staining, cell proliferation test and cell count. The results of hydrogen evolution corrosion test show that the corrosion resistance of mg Zn mn alloy and MgZnRe alloy are basically the same, and both of them are obviously superior to commercial AZ31B magnesium alloy. The results of blood compatibility showed that compared with the samples of AZ31B and 316L SS, the number of platelet adhesion on the surface of Mg-ZnMn alloy and MgZnRe alloy was less and there was almost no aggregation. The coagulation time of MgZnMn alloy and MgZnRe alloy was significantly longer than that of plasma, and the hemolysis rate of MgZnMn and MgZnRe were 4.7% and 4.4% respectively, which were lower than that of AZ31B alloy 80.9%. Therefore, MgZnMn and MgZnRe alloy had better blood compatibility. The results of apoptosis staining (A0/PI staining), cell proliferation evaluation (CCK-8) and cell counting showed that the cytocompatibility of MgZnRe alloy and MgZnRe alloy was significantly better than that of commercial AZ31B alloy.
【學位授予單位】：西南交通大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R318.08
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