【摘要】：高血脂是誘發(fā)心腦血管疾病和動(dòng)脈血管粥樣硬化等多種疾病的重要因素,因此調(diào)節(jié)體內(nèi)血脂水平是治療高血脂疾病的關(guān)鍵環(huán)節(jié)。對(duì)于血脂的治療,臨床上有抽脂手術(shù)、針灸治療、藥物治療、激光治療等治療方法。其中激光治療效果顯著,目前還沒有發(fā)現(xiàn)副作用。自從20世紀(jì)90年代之后,國(guó)內(nèi)開始出現(xiàn)低能量激光照射療法(Low-levelLaserTherapy,LLLT),此療法可對(duì)生物體的組織代謝系統(tǒng)、免疫系統(tǒng)以及血液循環(huán)系統(tǒng)進(jìn)行調(diào)節(jié),其原理是激光所產(chǎn)生的生物學(xué)效應(yīng),從而可以使患者從病理狀態(tài)恢復(fù)到正常狀態(tài)。本實(shí)驗(yàn)利用低能量激光照射對(duì)生物組織作用產(chǎn)生生物效應(yīng),激光照射離體血液后,觀察全血以及紅細(xì)胞對(duì)光譜吸收的變化,來分析血脂的改變,從而為低能量激光照射改善高血脂人群的血脂水平提供新的思路和方法。目的:本實(shí)驗(yàn)研究在650nm和405nm激光照射高血脂血樣和正常血樣的離體全血和紅細(xì)胞,觀察照射前后全血和紅細(xì)胞對(duì)光譜吸收的變化。方法:選取醫(yī)院中用全自動(dòng)生化分析儀測(cè)出的30例高血脂血樣和30例血脂正常血樣各4ml,將正常血樣設(shè)為正常組,高血脂血樣設(shè)為高血脂組。高血脂組與正常組分別用激光波長(zhǎng)650nm和405nm照射,調(diào)整激光參數(shù),使激光器輸出功率密度為20mW/cm2,激光能量密度為12J/cm2,照射15min,吸取0.5ml進(jìn)行紅細(xì)胞分離,分離的紅細(xì)胞與剩下的0.5ml全血樣本稀釋之后用酶標(biāo)儀進(jìn)行光譜掃描。用多功能酶標(biāo)儀掃描全血以及全血分離的紅細(xì)胞并繪制圖譜,統(tǒng)計(jì)全血及紅細(xì)胞吸收光譜的特性,比較正常組與高血脂組,激光照射前與照射后全血和紅細(xì)胞吸收光譜的不同之處,觀察吸收峰值的變化。結(jié)果:使用650nm激光照射高血脂組,對(duì)于全血統(tǒng)計(jì)中,照射后光譜吸收峰,比照射前高,在吸收峰416nm、544nm、578nm處,照射前后差異表現(xiàn)出統(tǒng)計(jì)學(xué)意義。對(duì)于紅細(xì)胞統(tǒng)計(jì)中,照射后紅細(xì)胞光譜吸收峰在416nm、544nm、578nm比照射前降低,且照射前后差異表現(xiàn)出統(tǒng)計(jì)學(xué)意義,其中在吸收峰416nm差異更為突出。使用650nm激光照射正常組,對(duì)于全血與紅細(xì)胞統(tǒng)計(jì)中,照射后紅細(xì)胞光譜吸收峰在416nm、544nm、578nm照射前后差異無統(tǒng)計(jì)學(xué)意義。使用405nm激光照射僅對(duì)高血脂紅細(xì)胞吸收光譜產(chǎn)生差異,對(duì)正常全血、紅細(xì)胞以及高血脂全血均未對(duì)其吸收光譜產(chǎn)生顯著性差異。結(jié)論:通過650nm與405nm低能量激光照射可以降低高血脂血液中紅細(xì)胞對(duì)光譜的吸收,間接反映出低能量激光在治療高血脂方面的療效。
[Abstract]:Hyperlipidemia is an important factor to induce cardiovascular and cerebrovascular diseases and atherosclerosis, so regulating the level of blood lipid is the key link in the treatment of hyperlipidemia. For the treatment of blood lipids, clinical liposuction surgery, acupuncture treatment, drug therapy, laser treatment and other treatment methods. Among them laser treatment effect is remarkable, no side effects have been found at present. Since the 1990s, Low-level LaserTherapyne LLLT (LLLT) has been introduced in China, which can regulate the tissue metabolism system, immune system and blood circulatory system of organism, which is based on the biological effect of laser. Thus, the patient can recover from pathological state to normal state. In this experiment, we used low energy laser irradiation to produce biological effect on biological tissue. After laser irradiation on isolated blood, we observed the changes of spectral absorption of whole blood and erythrocytes to analyze the changes of blood lipids. Therefore, it provides a new idea and method for low energy laser irradiation to improve the blood lipid level of hyperlipidemia population. Aim: to study the effects of 650nm and 405nm laser irradiation on whole blood and red blood cells of hyperlipidemia and normal blood samples, and observe the changes of spectral absorption of whole blood and red blood cells before and after irradiation. Methods: 30 cases of hyperlipidemia blood samples and 30 cases of normal blood samples of blood lipid were measured by automatic biochemical analyzer in hospital. The normal blood samples were divided into normal group and hyperlipidemia blood sample as hyperlipidemia group. In hyperlipidemia group and normal group, laser wavelength 650nm and 405nm were used to adjust laser parameters to make the laser output power density 20 MW / cm 2, laser energy density 12 J / cm 2, irradiation for 15 min, and 0.5ml was absorbed to separate red blood cells. The isolated red blood cells were diluted with the remaining 0.5ml whole blood samples and then scanned by an enzymatic spectrometer. The red blood cells separated from whole blood and whole blood were scanned by multifunctional enzyme standard instrument, and the characteristics of absorption spectrum of whole blood and red blood cell were analyzed and compared between normal group and hyperlipidemia group. The difference between the absorption spectra of whole blood and red blood cells before and after laser irradiation was observed. Results: in the whole blood statistics, the absorption peak after irradiation was higher than that before irradiation, and the difference before and after irradiation was statistically significant at 416nm ~ 544nm ~ 578nm. In erythrocyte statistics, the absorption peak of red blood cell at 544 nm after irradiation was lower than that before irradiation, and the difference before and after irradiation was statistically significant, especially in the absorption peak 416nm. In the whole blood and erythrocyte statistics, there was no significant difference between the whole blood and red blood cells before and after irradiation with 650nm laser irradiation at 416nm ~ 544nm ~ 578nm. The absorption spectra of hyperlipidemia red blood cells were only different by 405nm laser irradiation, but there was no significant difference on the absorption spectra of normal whole blood, red blood cells and hyperlipidemia whole blood. Conclusion: low energy laser irradiation with 650nm and 405nm can reduce the absorption of red blood cells in hyperlipidemia and indirectly reflect the therapeutic effect of low energy laser on hyperlipidemia.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R589.2;R454.2

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